Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cysts : methodological framework and applications
- Author
- Pjotr Meyvisch (UGent) , Pieter Gurdebeke (UGent) , Henk Vrielinck (UGent) , Kenneth Neil Mertens, Gerard Versteegh, Katarzyna Sliwinska and Stephen Louwye (UGent)
- Organization
- Abstract
- Fourier-transformed infrared (FTIR) spectroscopy is a spectrochemical technique able to retrieve macromolecular information from organic materials. When combined with a microscope (micro-FTIR), the (geo)chemical composition of single specimen dinoflagellate cysts (dinocysts) can be determined. Over the last decades a small number of dinocyst micro-FTIR studies booked often inconsistent results by overlooking important methodological aspects during analysis. This study takes into account variables like sample preparation, specimen morphology and size and spectral data processing steps and presents a standardized method based on attenuated total reflectance (ATR) micro-FTIR spectroscopy which is able to collect robust spectral datasets. These datasets are largely devoid of nonchemical artifacts inherent to other infrared spectrochemical methods which have typically been used in similar studies in the past (i.e. transmission and transflection spectroscopy). Several guidelines are proposed which facilitate the collection and qualitative interpretation of highly reproducible and repeatable spectrochemical dinocyst data. These, in turn, pave the way for a systematic exploration of dinocyst chemistry and its assessment as a chemotaxonomical tool or proxy. An ATR micro-FTIR case study on morphologically similar late Paleogene to early Neogene dinocysts Palaeocystodinium golzowense, Svalbardella clausii and Svalbardella cooksoniae is also presented, which highlights the chemotaxonomical potential of the method.
- Keywords
- Dinoflagellate cysts, Macromolecular composition, FTIR spectroscopy, Methodology, Chemotaxonomy
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01H11YA3FHRHTEY4TFAP5E3VYG
- MLA
- Meyvisch, Pjotr, et al. “Recent Advances in Infrared Spectroscopy Applied to Single Specimen Dinoflagellate Cysts : Methodological Framework and Applications.” 7th International Geologica Belgica Meeting 2021 : Geosciences Made in Belgium, Abstracts, 2021, pp. 249–249, doi:10.20341/gbcp.vol4.
- APA
- Meyvisch, P., Gurdebeke, P., Vrielinck, H., Mertens, K. N., Versteegh, G., Sliwinska, K., & Louwye, S. (2021). Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cysts : methodological framework and applications. 7th International Geologica Belgica Meeting 2021 : Geosciences Made in Belgium, Abstracts, 249–249. https://doi.org/10.20341/gbcp.vol4
- Chicago author-date
- Meyvisch, Pjotr, Pieter Gurdebeke, Henk Vrielinck, Kenneth Neil Mertens, Gerard Versteegh, Katarzyna Sliwinska, and Stephen Louwye. 2021. “Recent Advances in Infrared Spectroscopy Applied to Single Specimen Dinoflagellate Cysts : Methodological Framework and Applications.” In 7th International Geologica Belgica Meeting 2021 : Geosciences Made in Belgium, Abstracts, 249–249. https://doi.org/10.20341/gbcp.vol4.
- Chicago author-date (all authors)
- Meyvisch, Pjotr, Pieter Gurdebeke, Henk Vrielinck, Kenneth Neil Mertens, Gerard Versteegh, Katarzyna Sliwinska, and Stephen Louwye. 2021. “Recent Advances in Infrared Spectroscopy Applied to Single Specimen Dinoflagellate Cysts : Methodological Framework and Applications.” In 7th International Geologica Belgica Meeting 2021 : Geosciences Made in Belgium, Abstracts, 249–249. doi:10.20341/gbcp.vol4.
- Vancouver
- 1.Meyvisch P, Gurdebeke P, Vrielinck H, Mertens KN, Versteegh G, Sliwinska K, et al. Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cysts : methodological framework and applications. In: 7th International Geologica Belgica Meeting 2021 : Geosciences Made in Belgium, Abstracts. 2021. p. 249–249.
- IEEE
- [1]P. Meyvisch et al., “Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cysts : methodological framework and applications,” in 7th International Geologica Belgica Meeting 2021 : Geosciences Made in Belgium, Abstracts, Tervuren, Belgium, 2021, pp. 249–249.
@inproceedings{01H11YA3FHRHTEY4TFAP5E3VYG,
abstract = {{Fourier-transformed infrared (FTIR) spectroscopy is a spectrochemical technique able to retrieve macromolecular information from organic materials. When combined with a microscope (micro-FTIR), the (geo)chemical composition of single specimen dinoflagellate cysts (dinocysts) can be determined. Over the last decades a small number of dinocyst micro-FTIR studies booked often inconsistent results by overlooking important methodological aspects during analysis.
This study takes into account variables like sample preparation, specimen morphology and size and spectral data processing steps and presents a standardized method based on attenuated total reflectance (ATR) micro-FTIR spectroscopy which is able to collect robust spectral datasets. These datasets are largely devoid of nonchemical artifacts inherent to other infrared spectrochemical methods which have typically been used in similar studies in the past (i.e. transmission and transflection spectroscopy). Several guidelines are proposed which facilitate the collection and qualitative interpretation of highly reproducible and repeatable spectrochemical dinocyst data. These, in turn, pave the way for a systematic exploration of dinocyst chemistry and its assessment as a chemotaxonomical tool or proxy.
An ATR micro-FTIR case study on morphologically similar late Paleogene to early Neogene dinocysts Palaeocystodinium golzowense, Svalbardella clausii and Svalbardella cooksoniae is also presented, which highlights the chemotaxonomical potential of the method.}},
author = {{Meyvisch, Pjotr and Gurdebeke, Pieter and Vrielinck, Henk and Mertens, Kenneth Neil and Versteegh, Gerard and Sliwinska, Katarzyna and Louwye, Stephen}},
booktitle = {{7th International Geologica Belgica Meeting 2021 : Geosciences Made in Belgium, Abstracts}},
keywords = {{Dinoflagellate cysts,Macromolecular composition,FTIR spectroscopy,Methodology,Chemotaxonomy}},
language = {{eng}},
location = {{Tervuren, Belgium}},
pages = {{249--249}},
title = {{Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cysts : methodological framework and applications}},
url = {{http://doi.org/10.20341/gbcp.vol4}},
year = {{2021}},
}
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