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Differential protease content of mast cells and the processing of IL-33 in Alternaria alternata induced allergic airway inflammation in mice

Olga Krysko (UGent) , Darya Korsakova, Andrea Renate Teufelberger, Amse De Meyer (UGent) , Jill Steels (UGent) , Natalie De Ruyck (UGent) , Judith van Ovost (UGent) , Sharon Van Nevel, Gabriële Holtappels (UGent) , Frauke Coppieters (UGent) , et al.
(2023) FRONTIERS IN IMMUNOLOGY. 14.
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Abstract
Background: Recent in vitro studies strongly implicated mast cell-derived proteases as regulators of IL-33 activity by enzymatic cleavage in its central domain. A better understanding of the role of mast cell proteases on IL-33 activity in vivo is needed. We aimed to compare the expression of mast cell proteases in C57BL/6 and BALB/c mice, their role in the cleavage of IL-33 cytokine, and their contribution to allergic airway inflammation.; Results: In vitro, full-length IL-33 protein was efficiently degraded by mast cell supernatants of BALB/c mice in contrast to the mast cell supernatants from C57BL/6 mice. RNAseq analysis indicated major differences in the gene expression profiles of bone marrow-derived mast cells from C57BL/6 and BALB/c mice. In Alternaria alternata (Alt) - treated C57BL/6 mice the full-length form of IL-33 was mainly present, while in BALB/c mice, the processed shorter form of IL-33 was more prominent. The observed cleavage pattern of IL-33 was associated with a nearly complete lack of mast cells and their proteases in the lungs of C57BL/6 mice. While most inflammatory cells were similarly increased in Alt-treated C57BL/6 and BALB/c mice, C57BL/6 mice had significantly more eosinophils in the bronchoalveolar lavage fluid and IL-5 protein levels in their lungs than BALB/c mice.; Conclusion: Our study demonstrates that lung mast cells differ in number and protease content between the two tested mouse strains and could affect the processing of IL-33 and inflammatory outcome of Alt -induced airway inflammation. We suggest that mast cells and their proteases play a regulatory role in IL-33-induced lung inflammation by limiting its proinflammatory effect via the IL-33/ST2 signaling pathway.
Keywords
IL-33, allergy, mast cells, protease, type 2 inflammation, allergic airway inflammation, Alternaria alternata, C57BL/6, RNAseq, BALB/c, lung inflammation

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MLA
Krysko, Olga, et al. “Differential Protease Content of Mast Cells and the Processing of IL-33 in Alternaria Alternata Induced Allergic Airway Inflammation in Mice.” FRONTIERS IN IMMUNOLOGY, vol. 14, 2023, doi:10.3389/fimmu.2023.1040493.
APA
Krysko, O., Korsakova, D., Teufelberger, A. R., De Meyer, A., Steels, J., De Ruyck, N., … Bachert, C. (2023). Differential protease content of mast cells and the processing of IL-33 in Alternaria alternata induced allergic airway inflammation in mice. FRONTIERS IN IMMUNOLOGY, 14. https://doi.org/10.3389/fimmu.2023.1040493
Chicago author-date
Krysko, Olga, Darya Korsakova, Andrea Renate Teufelberger, Amse De Meyer, Jill Steels, Natalie De Ruyck, Judith van Ovost, et al. 2023. “Differential Protease Content of Mast Cells and the Processing of IL-33 in Alternaria Alternata Induced Allergic Airway Inflammation in Mice.” FRONTIERS IN IMMUNOLOGY 14. https://doi.org/10.3389/fimmu.2023.1040493.
Chicago author-date (all authors)
Krysko, Olga, Darya Korsakova, Andrea Renate Teufelberger, Amse De Meyer, Jill Steels, Natalie De Ruyck, Judith van Ovost, Sharon Van Nevel, Gabriële Holtappels, Frauke Coppieters, Mikhail Ivanchenko, Harald Braun, Maria Vedunova, Dmitri Krysko, and Claus Bachert. 2023. “Differential Protease Content of Mast Cells and the Processing of IL-33 in Alternaria Alternata Induced Allergic Airway Inflammation in Mice.” FRONTIERS IN IMMUNOLOGY 14. doi:10.3389/fimmu.2023.1040493.
Vancouver
1.
Krysko O, Korsakova D, Teufelberger AR, De Meyer A, Steels J, De Ruyck N, et al. Differential protease content of mast cells and the processing of IL-33 in Alternaria alternata induced allergic airway inflammation in mice. FRONTIERS IN IMMUNOLOGY. 2023;14.
IEEE
[1]
O. Krysko et al., “Differential protease content of mast cells and the processing of IL-33 in Alternaria alternata induced allergic airway inflammation in mice,” FRONTIERS IN IMMUNOLOGY, vol. 14, 2023.
@article{01H051EHYH3ZP1P37WGH8WJJS5,
  abstract     = {{Background: Recent in vitro studies strongly implicated mast cell-derived proteases as regulators of IL-33 activity by enzymatic cleavage in its central domain. A better understanding of the role of mast cell proteases on IL-33 activity in vivo is needed. We aimed to compare the expression of mast cell proteases in C57BL/6 and BALB/c mice, their role in the cleavage of IL-33 cytokine, and their contribution to allergic airway inflammation.; Results: In vitro, full-length IL-33 protein was efficiently degraded by mast cell supernatants of BALB/c mice in contrast to the mast cell supernatants from C57BL/6 mice. RNAseq analysis indicated major differences in the gene expression profiles of bone marrow-derived mast cells from C57BL/6 and BALB/c mice. In Alternaria alternata (Alt) - treated C57BL/6 mice the full-length form of IL-33 was mainly present, while in BALB/c mice, the processed shorter form of IL-33 was more prominent. The observed cleavage pattern of IL-33 was associated with a nearly complete lack of mast cells and their proteases in the lungs of C57BL/6 mice. While most inflammatory cells were similarly increased in Alt-treated C57BL/6 and BALB/c mice, C57BL/6 mice had significantly more eosinophils in the bronchoalveolar lavage fluid and IL-5 protein levels in their lungs than BALB/c mice.; Conclusion: Our study demonstrates that lung mast cells differ in number and protease content between the two tested mouse strains and could affect the processing of IL-33 and inflammatory outcome of Alt -induced airway inflammation. We suggest that mast cells and their proteases play a regulatory role in IL-33-induced lung inflammation by limiting its proinflammatory effect via the IL-33/ST2 signaling pathway. }},
  articleno    = {{1040493}},
  author       = {{Krysko, Olga and  Korsakova, Darya and Teufelberger, Andrea Renate and De Meyer, Amse and Steels, Jill and De Ruyck, Natalie and van Ovost, Judith and Van Nevel, Sharon and Holtappels, Gabriële and Coppieters, Frauke and  Ivanchenko, Mikhail and Braun, Harald and  Vedunova, Maria and Krysko, Dmitri and Bachert, Claus}},
  issn         = {{1664-3224}},
  journal      = {{FRONTIERS IN IMMUNOLOGY}},
  keywords     = {{IL-33,allergy,mast cells,protease,type 2 inflammation,allergic airway inflammation,Alternaria alternata,C57BL/6,RNAseq,BALB/c,lung inflammation}},
  language     = {{eng}},
  pages        = {{14}},
  title        = {{Differential protease content of mast cells and the processing of IL-33 in Alternaria alternata induced allergic airway inflammation in mice}},
  url          = {{http://doi.org/10.3389/fimmu.2023.1040493}},
  volume       = {{14}},
  year         = {{2023}},
}
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