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Development of a microfluidic chip powered by EWOD for in vitro manipulation of bovine embryos

Adriana Karcz (UGent) , Ann Van Soom (UGent) , Katrien Smits (UGent) , Sandra Van Vlierberghe (UGent) , Rik Verplancke (UGent) , Osvaldo Américo Bogado Pascottini (UGent) , Etienne Van den Abbeel (UGent) and Jan Vanfleteren (UGent)
(2023) BIOSENSORS-BASEL. 13(4).
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Abstract
Digital microfluidics (DMF) holds great potential for the alleviation of laboratory procedures in assisted reproductive technologies (ARTs). The electrowetting on dielectric (EWOD) technology provides dynamic culture conditions in vitro that may better mimic the natural embryo microenvironment. Thus far, EWOD microdevices have been proposed for in vitro gamete and embryo handling in mice and for analyzing the human embryo secretome. This article presents the development of the first microfluidic chip utilizing EWOD technology designed for the manipulation of bovine embryos in vitro. The prototype sustains the cell cycles of embryos manipulated individually on the chips during in vitro culture (IVC). Challenges related to the chip fabrication as well as to its application during bovine embryo IVC in accordance with the adapted on-chip protocol are thoroughly discussed, and future directions for DMF in ARTs are indicated.
Keywords
microfluidics, electrowetting on dielectric, individual embryo culture, lab-on-a-chip, cell manipulation, digital microfluidics

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MLA
Karcz, Adriana, et al. “Development of a Microfluidic Chip Powered by EWOD for in Vitro Manipulation of Bovine Embryos.” BIOSENSORS-BASEL, vol. 13, no. 4, 2023, doi:10.3390/bios13040419.
APA
Karcz, A., Van Soom, A., Smits, K., Van Vlierberghe, S., Verplancke, R., Bogado Pascottini, O. A., … Vanfleteren, J. (2023). Development of a microfluidic chip powered by EWOD for in vitro manipulation of bovine embryos. BIOSENSORS-BASEL, 13(4). https://doi.org/10.3390/bios13040419
Chicago author-date
Karcz, Adriana, Ann Van Soom, Katrien Smits, Sandra Van Vlierberghe, Rik Verplancke, Osvaldo Américo Bogado Pascottini, Etienne Van den Abbeel, and Jan Vanfleteren. 2023. “Development of a Microfluidic Chip Powered by EWOD for in Vitro Manipulation of Bovine Embryos.” BIOSENSORS-BASEL 13 (4). https://doi.org/10.3390/bios13040419.
Chicago author-date (all authors)
Karcz, Adriana, Ann Van Soom, Katrien Smits, Sandra Van Vlierberghe, Rik Verplancke, Osvaldo Américo Bogado Pascottini, Etienne Van den Abbeel, and Jan Vanfleteren. 2023. “Development of a Microfluidic Chip Powered by EWOD for in Vitro Manipulation of Bovine Embryos.” BIOSENSORS-BASEL 13 (4). doi:10.3390/bios13040419.
Vancouver
1.
Karcz A, Van Soom A, Smits K, Van Vlierberghe S, Verplancke R, Bogado Pascottini OA, et al. Development of a microfluidic chip powered by EWOD for in vitro manipulation of bovine embryos. BIOSENSORS-BASEL. 2023;13(4).
IEEE
[1]
A. Karcz et al., “Development of a microfluidic chip powered by EWOD for in vitro manipulation of bovine embryos,” BIOSENSORS-BASEL, vol. 13, no. 4, 2023.
@article{01H032HZGG0CFB0CA1JCBXA551,
  abstract     = {{Digital microfluidics (DMF) holds great potential for the alleviation of laboratory procedures in assisted reproductive technologies (ARTs). The electrowetting on dielectric (EWOD) technology provides dynamic culture conditions in vitro that may better mimic the natural embryo microenvironment. Thus far, EWOD microdevices have been proposed for in vitro gamete and embryo handling in mice and for analyzing the human embryo secretome. This article presents the development of the first microfluidic chip utilizing EWOD technology designed for the manipulation of bovine embryos in vitro. The prototype sustains the cell cycles of embryos manipulated individually on the chips during in vitro culture (IVC). Challenges related to the chip fabrication as well as to its application during bovine embryo IVC in accordance with the adapted on-chip protocol are thoroughly discussed, and future directions for DMF in ARTs are indicated.}},
  articleno    = {{419}},
  author       = {{Karcz, Adriana and Van Soom, Ann and Smits, Katrien and Van Vlierberghe, Sandra and Verplancke, Rik and Bogado Pascottini, Osvaldo Américo and Van den Abbeel, Etienne and Vanfleteren, Jan}},
  issn         = {{2079-6374}},
  journal      = {{BIOSENSORS-BASEL}},
  keywords     = {{microfluidics,electrowetting on dielectric,individual embryo culture,lab-on-a-chip,cell manipulation,digital microfluidics}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{19}},
  title        = {{Development of a microfluidic chip powered by EWOD for in vitro manipulation of bovine embryos}},
  url          = {{http://doi.org/10.3390/bios13040419}},
  volume       = {{13}},
  year         = {{2023}},
}
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