Advanced search
1 file | 5.23 MB Add to list

New insights into the combined toxicity of aflatoxin B1 and fumonisin B1 in HepG2 cells using Seahorse respirometry analysis and RNA transcriptome sequencing

Author
Organization
Project
Abstract
Aflatoxin B1 (AFB1) and fumonisin B1 (FB1) are widely (co-)detected in food and known for their hepatotoxicity in humans. Still, their combined toxicity needs to be investigated, especially the impact on mitochondria. In our previous work, we examined the effect of short-term exposure to different doses of AFB1, FB1, and their binary mixture (MIX) on the bioenergetic status of HepG2 cells, a well-recognized in vitro model system for studying liver cell function. In the current work, we further investigated the (combined) effect of AFB1 and FB1 on the mitochondrial and glycolytic activity of HepG2 cells using Seahorse respirometry analysis and RNA transcriptome sequencing. The results showed that the co-exposure, especially at high doses, is more toxic due to a more inhibition of all parameters of mitochondrial respiration. However, FB1 contributes more to the MIX effects than AFB1. RNA transcriptome sequencing showed that the p53 signaling pathway, a major orchestrator of mitochondrial apoptosis, was differentially expressed. Moreover, the co-exposure significantly downregulated the genes encoding for Complexes I, II, III, and IV, representing the onset of the suppressed mitochondrial respiration in HepG2 cells.
Keywords
Aflatoxin B1, Fumonisin B1, Bioenergetics, Seahorse analysis, p53, Mitochondrial toxicity, Apoptosis, Transcriptomics

Downloads

  • 1-s2.0-S0160412023002180-main.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 5.23 MB

Citation

Please use this url to cite or link to this publication:

MLA
Chen, Xiangrong, et al. “New Insights into the Combined Toxicity of Aflatoxin B1 and Fumonisin B1 in HepG2 Cells Using Seahorse Respirometry Analysis and RNA Transcriptome Sequencing.” ENVIRONMENT INTERNATIONAL, vol. 175, 2023, doi:10.1016/j.envint.2023.107945.
APA
Chen, X., Abdallah, M. F., Grootaert, C., Van Nieuwerburgh, F., & Rajkovic, A. (2023). New insights into the combined toxicity of aflatoxin B1 and fumonisin B1 in HepG2 cells using Seahorse respirometry analysis and RNA transcriptome sequencing. ENVIRONMENT INTERNATIONAL, 175. https://doi.org/10.1016/j.envint.2023.107945
Chicago author-date
Chen, Xiangrong, Mohamed Fathi Abdallah, Charlotte Grootaert, Filip Van Nieuwerburgh, and Andreja Rajkovic. 2023. “New Insights into the Combined Toxicity of Aflatoxin B1 and Fumonisin B1 in HepG2 Cells Using Seahorse Respirometry Analysis and RNA Transcriptome Sequencing.” ENVIRONMENT INTERNATIONAL 175. https://doi.org/10.1016/j.envint.2023.107945.
Chicago author-date (all authors)
Chen, Xiangrong, Mohamed Fathi Abdallah, Charlotte Grootaert, Filip Van Nieuwerburgh, and Andreja Rajkovic. 2023. “New Insights into the Combined Toxicity of Aflatoxin B1 and Fumonisin B1 in HepG2 Cells Using Seahorse Respirometry Analysis and RNA Transcriptome Sequencing.” ENVIRONMENT INTERNATIONAL 175. doi:10.1016/j.envint.2023.107945.
Vancouver
1.
Chen X, Abdallah MF, Grootaert C, Van Nieuwerburgh F, Rajkovic A. New insights into the combined toxicity of aflatoxin B1 and fumonisin B1 in HepG2 cells using Seahorse respirometry analysis and RNA transcriptome sequencing. ENVIRONMENT INTERNATIONAL. 2023;175.
IEEE
[1]
X. Chen, M. F. Abdallah, C. Grootaert, F. Van Nieuwerburgh, and A. Rajkovic, “New insights into the combined toxicity of aflatoxin B1 and fumonisin B1 in HepG2 cells using Seahorse respirometry analysis and RNA transcriptome sequencing,” ENVIRONMENT INTERNATIONAL, vol. 175, 2023.
@article{01GYZ2SE796H2W69TAN2S5QSB2,
  abstract     = {{Aflatoxin B1 (AFB1) and fumonisin B1 (FB1) are widely (co-)detected in food and known for their hepatotoxicity in humans. Still, their combined toxicity needs to be investigated, especially the impact on mitochondria. In our previous work, we examined the effect of short-term exposure to different doses of AFB1, FB1, and their binary mixture (MIX) on the bioenergetic status of HepG2 cells, a well-recognized in vitro model system for studying liver cell function. In the current work, we further investigated the (combined) effect of AFB1 and FB1 on the mitochondrial and glycolytic activity of HepG2 cells using Seahorse respirometry analysis and RNA transcriptome sequencing. The results showed that the co-exposure, especially at high doses, is more toxic due to a more inhibition of all parameters of mitochondrial respiration. However, FB1 contributes more to the MIX effects than AFB1. RNA transcriptome sequencing showed that the p53 signaling pathway, a major orchestrator of mitochondrial apoptosis, was differentially expressed. Moreover, the co-exposure significantly downregulated the genes encoding for Complexes I, II, III, and IV, representing the onset of the suppressed mitochondrial respiration in HepG2 cells.}},
  articleno    = {{107945}},
  author       = {{Chen, Xiangrong and Abdallah, Mohamed Fathi and Grootaert, Charlotte and Van Nieuwerburgh, Filip and Rajkovic, Andreja}},
  issn         = {{0160-4120}},
  journal      = {{ENVIRONMENT INTERNATIONAL}},
  keywords     = {{Aflatoxin B1,Fumonisin B1,Bioenergetics,Seahorse analysis,p53,Mitochondrial toxicity,Apoptosis,Transcriptomics}},
  language     = {{eng}},
  pages        = {{12}},
  title        = {{New insights into the combined toxicity of aflatoxin B1 and fumonisin B1 in HepG2 cells using Seahorse respirometry analysis and RNA transcriptome sequencing}},
  url          = {{http://doi.org/10.1016/j.envint.2023.107945}},
  volume       = {{175}},
  year         = {{2023}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: