Single-cell profiling identifies a novel human polyclonal unconventional T cell lineage
- Author
- Lore Billiet (UGent) , Laurenz De Cock (UGent) , Guillem Sanchez Sanchez, Rupert Mayer (UGent) , Glenn Goetgeluk (UGent) , Stijn De Munter (UGent) , Melissa Pille (UGent) , Joline Ingels (UGent) , Hanne Jansen (UGent) , Karin Weening (UGent) , Eva Pascal (UGent) , Killian Raes (UGent) , Sarah Bonte (UGent) , Tessa Kerre (UGent) , Niels Vandamme (UGent) , Ruth Seurinck (UGent) , Jana Roels (UGent) , Marieke Lavaert, Filip Van Nieuwerburgh (UGent) , Georges Leclercq (UGent) , Tom Taghon (UGent) , Francis Impens (UGent) , Björn Menten (UGent) , David Vermijlen and Bart Vandekerckhove (UGent)
- Organization
- Abstract
- Billiet et al. identify the post-thymic progeny of the agonist-selected CD10(+) PD-1(+) precursors in humans based on shared characteristics of the T cell receptor repertoire and the transcriptome. This lineage represents a well-defined but heterogeneous, unconventional TCR alpha beta(+) lineage mostly confined within the CD8(+) Helios(+) T cells. In the human thymus, a CD10(+) PD-1(+) TCR alpha beta(+) differentiation pathway diverges from the conventional single positive T cell lineages at the early double-positive stage. Here, we identify the progeny of this unconventional lineage in antigen-inexperienced blood. These unconventional T cells (UTCs) in thymus and blood share a transcriptomic profile, characterized by hallmark transcription factors (i.e., ZNF683 and IKZF2), and a polyclonal TCR repertoire with autoreactive features, exhibiting a bias toward early TCR alpha chain rearrangements. Single-cell RNA sequencing confirms a common developmental trajectory between the thymic and blood UTCs and clearly delineates this unconventional lineage in blood. Besides MME+ recent thymic emigrants, effector-like clusters are identified in this heterogeneous lineage. Expression of Helios and KIR and a decreased CD8 beta expression are characteristics of this lineage. This UTC lineage could be identified in adult blood and intestinal tissues. In summary, our data provide a comprehensive characterization of the polyclonal unconventional lineage in antigen-inexperienced blood and identify the adult progeny.
- Keywords
- Immunology, Immunology and Allergy
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GYYXFX77KJ2AKMCZYSM8WVV3
- MLA
- Billiet, Lore, et al. “Single-Cell Profiling Identifies a Novel Human Polyclonal Unconventional T Cell Lineage.” JOURNAL OF EXPERIMENTAL MEDICINE, vol. 220, no. 6, Rockefeller University Press, 2023, doi:10.1084/jem.20220942.
- APA
- Billiet, L., De Cock, L., Sanchez Sanchez, G., Mayer, R., Goetgeluk, G., De Munter, S., … Vandekerckhove, B. (2023). Single-cell profiling identifies a novel human polyclonal unconventional T cell lineage. JOURNAL OF EXPERIMENTAL MEDICINE, 220(6). https://doi.org/10.1084/jem.20220942
- Chicago author-date
- Billiet, Lore, Laurenz De Cock, Guillem Sanchez Sanchez, Rupert Mayer, Glenn Goetgeluk, Stijn De Munter, Melissa Pille, et al. 2023. “Single-Cell Profiling Identifies a Novel Human Polyclonal Unconventional T Cell Lineage.” JOURNAL OF EXPERIMENTAL MEDICINE 220 (6). https://doi.org/10.1084/jem.20220942.
- Chicago author-date (all authors)
- Billiet, Lore, Laurenz De Cock, Guillem Sanchez Sanchez, Rupert Mayer, Glenn Goetgeluk, Stijn De Munter, Melissa Pille, Joline Ingels, Hanne Jansen, Karin Weening, Eva Pascal, Killian Raes, Sarah Bonte, Tessa Kerre, Niels Vandamme, Ruth Seurinck, Jana Roels, Marieke Lavaert, Filip Van Nieuwerburgh, Georges Leclercq, Tom Taghon, Francis Impens, Björn Menten, David Vermijlen, and Bart Vandekerckhove. 2023. “Single-Cell Profiling Identifies a Novel Human Polyclonal Unconventional T Cell Lineage.” JOURNAL OF EXPERIMENTAL MEDICINE 220 (6). doi:10.1084/jem.20220942.
- Vancouver
- 1.Billiet L, De Cock L, Sanchez Sanchez G, Mayer R, Goetgeluk G, De Munter S, et al. Single-cell profiling identifies a novel human polyclonal unconventional T cell lineage. JOURNAL OF EXPERIMENTAL MEDICINE. 2023;220(6).
- IEEE
- [1]L. Billiet et al., “Single-cell profiling identifies a novel human polyclonal unconventional T cell lineage,” JOURNAL OF EXPERIMENTAL MEDICINE, vol. 220, no. 6, 2023.
@article{01GYYXFX77KJ2AKMCZYSM8WVV3,
abstract = {{Billiet et al. identify the post-thymic progeny of the agonist-selected CD10(+) PD-1(+) precursors in humans based on shared characteristics of the T cell receptor repertoire and the transcriptome. This lineage represents a well-defined but heterogeneous, unconventional TCR alpha beta(+) lineage mostly confined within the CD8(+) Helios(+) T cells.
In the human thymus, a CD10(+) PD-1(+) TCR alpha beta(+) differentiation pathway diverges from the conventional single positive T cell lineages at the early double-positive stage. Here, we identify the progeny of this unconventional lineage in antigen-inexperienced blood. These unconventional T cells (UTCs) in thymus and blood share a transcriptomic profile, characterized by hallmark transcription factors (i.e., ZNF683 and IKZF2), and a polyclonal TCR repertoire with autoreactive features, exhibiting a bias toward early TCR alpha chain rearrangements. Single-cell RNA sequencing confirms a common developmental trajectory between the thymic and blood UTCs and clearly delineates this unconventional lineage in blood. Besides MME+ recent thymic emigrants, effector-like clusters are identified in this heterogeneous lineage. Expression of Helios and KIR and a decreased CD8 beta expression are characteristics of this lineage. This UTC lineage could be identified in adult blood and intestinal tissues. In summary, our data provide a comprehensive characterization of the polyclonal unconventional lineage in antigen-inexperienced blood and identify the adult progeny.}},
articleno = {{e20220942}},
author = {{Billiet, Lore and De Cock, Laurenz and Sanchez Sanchez, Guillem and Mayer, Rupert and Goetgeluk, Glenn and De Munter, Stijn and Pille, Melissa and Ingels, Joline and Jansen, Hanne and Weening, Karin and Pascal, Eva and Raes, Killian and Bonte, Sarah and Kerre, Tessa and Vandamme, Niels and Seurinck, Ruth and Roels, Jana and Lavaert, Marieke and Van Nieuwerburgh, Filip and Leclercq, Georges and Taghon, Tom and Impens, Francis and Menten, Björn and Vermijlen, David and Vandekerckhove, Bart}},
issn = {{0022-1007}},
journal = {{JOURNAL OF EXPERIMENTAL MEDICINE}},
keywords = {{Immunology,Immunology and Allergy}},
language = {{eng}},
number = {{6}},
pages = {{30}},
publisher = {{Rockefeller University Press}},
title = {{Single-cell profiling identifies a novel human polyclonal unconventional T cell lineage}},
url = {{http://doi.org/10.1084/jem.20220942}},
volume = {{220}},
year = {{2023}},
}
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