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Cathepsin-L secreted by high-quality bovine embryos exerts an embryotrophic effect in vitro

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Abstract
While human in vitro embryo production is generally performed individually, animal models have shown that culturing embryos in groups improves blastocyst yield and quality. Paracrine embryotrophins could be responsible for this improved embryo development, but their identity remains largely unknown. We hypothesize that supplementation of embryotrophic proteins to a culture medium could be the key to improve individual embryo production. In this study, proteomics screening of culture media conditioned by bovine embryos revealed cathepsin-L as being secreted by both excellent- and good-quality embryos, while being absent in the medium conditioned by poor-quality embryos. The embryotrophic role of cathepsin-L was explored in vitro, whereby bovine zygotes were cultured individually for 8 days with or without cathepsin-L. Preliminary dose–response experiments pointed out 100 ng/mL as the optimal concentration of cathepsin-L in embryo culture medium. Supplementation of cathepsin-L to individual culture systems significantly improved blastocyst development and quality in terms of blastocoel formation at day 7, and the hatching ratio and apoptotic cell ratio at day 8, compared to the control. Taken together, cathepsin-L acts as an important embryotrophin by increasing embryo quality, and regulating blastulation and hatching in bovine in vitro embryo production.
Keywords
Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, Catalysis, embryotrophins, embryonic development, in vitro culture

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MLA
Raes, Annelies, et al. “Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect in Vitro.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 7, 2023, doi:10.3390/ijms24076563.
APA
Raes, A., Wydooghe, E., Pavani, K., Bogado Pascottini, O. A., Van Steendam, K., Dhaenens, M., … Smits, K. (2023). Cathepsin-L secreted by high-quality bovine embryos exerts an embryotrophic effect in vitro. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(7). https://doi.org/10.3390/ijms24076563
Chicago author-date
Raes, Annelies, Eline Wydooghe, Krishna Pavani, Osvaldo Américo Bogado Pascottini, Katleen Van Steendam, Maarten Dhaenens, Annekatrien Boel, et al. 2023. “Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect in Vitro.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (7). https://doi.org/10.3390/ijms24076563.
Chicago author-date (all authors)
Raes, Annelies, Eline Wydooghe, Krishna Pavani, Osvaldo Américo Bogado Pascottini, Katleen Van Steendam, Maarten Dhaenens, Annekatrien Boel, Sonia Heras, Björn Heindryckx, Luc Peelman, Dieter Deforce, Filip Van Nieuwerburgh, Geert Opsomer, Ann Van Soom, and Katrien Smits. 2023. “Cathepsin-L Secreted by High-Quality Bovine Embryos Exerts an Embryotrophic Effect in Vitro.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (7). doi:10.3390/ijms24076563.
Vancouver
1.
Raes A, Wydooghe E, Pavani K, Bogado Pascottini OA, Van Steendam K, Dhaenens M, et al. Cathepsin-L secreted by high-quality bovine embryos exerts an embryotrophic effect in vitro. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2023;24(7).
IEEE
[1]
A. Raes et al., “Cathepsin-L secreted by high-quality bovine embryos exerts an embryotrophic effect in vitro,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 7, 2023.
@article{01GYYB4WA031H8P9EZWTWR1XFQ,
  abstract     = {{While human in vitro embryo production is generally performed individually, animal models have shown that culturing embryos in groups improves blastocyst yield and quality. Paracrine embryotrophins could be responsible for this improved embryo development, but their identity remains largely unknown. We hypothesize that supplementation of embryotrophic proteins to a culture medium could be the key to improve individual embryo production. In this study, proteomics screening of culture media conditioned by bovine embryos revealed cathepsin-L as being secreted by both excellent- and good-quality embryos, while being absent in the medium conditioned by poor-quality embryos. The embryotrophic role of cathepsin-L was explored in vitro, whereby bovine zygotes were cultured individually for 8 days with or without cathepsin-L. Preliminary dose–response experiments pointed out 100 ng/mL as the optimal concentration of cathepsin-L in embryo culture medium. Supplementation of cathepsin-L to individual culture systems significantly improved blastocyst development and quality in terms of blastocoel formation at day 7, and the hatching ratio and apoptotic cell ratio at day 8, compared to the control. Taken together, cathepsin-L acts as an important embryotrophin by increasing embryo quality, and regulating blastulation and hatching in bovine in vitro embryo production.}},
  articleno    = {{6563}},
  author       = {{Raes, Annelies and Wydooghe, Eline and Pavani, Krishna and Bogado Pascottini, Osvaldo Américo and Van Steendam, Katleen and Dhaenens, Maarten and Boel, Annekatrien and Heras, Sonia and Heindryckx, Björn and Peelman, Luc and Deforce, Dieter and Van Nieuwerburgh, Filip and Opsomer, Geert and Van Soom, Ann and Smits, Katrien}},
  issn         = {{1661-6596}},
  journal      = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
  keywords     = {{Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis,embryotrophins,embryonic development,in vitro culture}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{17}},
  title        = {{Cathepsin-L secreted by high-quality bovine embryos exerts an embryotrophic effect in vitro}},
  url          = {{http://doi.org/10.3390/ijms24076563}},
  volume       = {{24}},
  year         = {{2023}},
}

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