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Dicer-like 3a mediates intergenerational resistance against root-knot nematodes in rice via hormone responses

Aniko Meijer, Mohammad Atighi Quchan Atigh, Kristof Demeestere (UGent) , Tim De Meyer (UGent) , Klaas Vandepoele (UGent) and Tina Kyndt (UGent)
(2023) PLANT PHYSIOLOGY. 193(3). p.2071-2085
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Abstract
In a continuously changing and challenging environment, passing down the memory of encountered stress factors to offspring could provide an evolutionary advantage. In this study, we demonstrate the existence of 'intergenerational acquired resistance' in the progeny of rice (Oryza sativa) plants attacked by the belowground parasitic nematode Meloidogyne graminicola. Transcriptome analyses revealed that genes involved in defense pathways are generally downregulated in progeny of nematode-infected plants under uninfected conditions but show a stronger induction upon nematode infection. This phenomenon was termed "spring loading" and depends on initial downregulation by the 24nt siRNA biogenesis gene dicer-like 3a (dcl3a) involved in the RNA-directed DNA methylation pathway. Knock-down of dcl3a led to increased nematode susceptibility and abolished intergenerational acquired resistance, as well as jasmonic acid/ethylene spring loading in the offspring of infected plants. The importance of ethylene signaling in intergenerational resistance was confirmed by experiments on a knock-down line of ethylene insensitive 2 (ein2b), which lacks intergenerational acquired resistance. Taken together, these data indicate a role for DCL3a in regulating plant defense pathways during both within-generation and intergenerational resistance against nematodes in rice.
Keywords
Plant Science, Genetics, Physiology

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Citation

Please use this url to cite or link to this publication:

MLA
Meijer, Aniko, et al. “Dicer-like 3a Mediates Intergenerational Resistance against Root-Knot Nematodes in Rice via Hormone Responses.” PLANT PHYSIOLOGY, vol. 193, no. 3, 2023, pp. 2071–85, doi:10.1093/plphys/kiad215.
APA
Meijer, A., Atighi Quchan Atigh, M., Demeestere, K., De Meyer, T., Vandepoele, K., & Kyndt, T. (2023). Dicer-like 3a mediates intergenerational resistance against root-knot nematodes in rice via hormone responses. PLANT PHYSIOLOGY, 193(3), 2071–2085. https://doi.org/10.1093/plphys/kiad215
Chicago author-date
Meijer, Aniko, Mohammad Atighi Quchan Atigh, Kristof Demeestere, Tim De Meyer, Klaas Vandepoele, and Tina Kyndt. 2023. “Dicer-like 3a Mediates Intergenerational Resistance against Root-Knot Nematodes in Rice via Hormone Responses.” PLANT PHYSIOLOGY 193 (3): 2071–85. https://doi.org/10.1093/plphys/kiad215.
Chicago author-date (all authors)
Meijer, Aniko, Mohammad Atighi Quchan Atigh, Kristof Demeestere, Tim De Meyer, Klaas Vandepoele, and Tina Kyndt. 2023. “Dicer-like 3a Mediates Intergenerational Resistance against Root-Knot Nematodes in Rice via Hormone Responses.” PLANT PHYSIOLOGY 193 (3): 2071–2085. doi:10.1093/plphys/kiad215.
Vancouver
1.
Meijer A, Atighi Quchan Atigh M, Demeestere K, De Meyer T, Vandepoele K, Kyndt T. Dicer-like 3a mediates intergenerational resistance against root-knot nematodes in rice via hormone responses. PLANT PHYSIOLOGY. 2023;193(3):2071–85.
IEEE
[1]
A. Meijer, M. Atighi Quchan Atigh, K. Demeestere, T. De Meyer, K. Vandepoele, and T. Kyndt, “Dicer-like 3a mediates intergenerational resistance against root-knot nematodes in rice via hormone responses,” PLANT PHYSIOLOGY, vol. 193, no. 3, pp. 2071–2085, 2023.
@article{01GXZGFS53YK0T9VAJJRT1DD1G,
  abstract     = {{In a continuously changing and challenging environment, passing down the memory of encountered stress factors to offspring could provide an evolutionary advantage. In this study, we demonstrate the existence of 'intergenerational acquired resistance' in the progeny of rice (Oryza sativa) plants attacked by the belowground parasitic nematode Meloidogyne graminicola. Transcriptome analyses revealed that genes involved in defense pathways are generally downregulated in progeny of nematode-infected plants under uninfected conditions but show a stronger induction upon nematode infection. This phenomenon was termed "spring loading" and depends on initial downregulation by the 24nt siRNA biogenesis gene dicer-like 3a (dcl3a) involved in the RNA-directed DNA methylation pathway. Knock-down of dcl3a led to increased nematode susceptibility and abolished intergenerational acquired resistance, as well as jasmonic acid/ethylene spring loading in the offspring of infected plants. The importance of ethylene signaling in intergenerational resistance was confirmed by experiments on a knock-down line of ethylene insensitive 2 (ein2b), which lacks intergenerational acquired resistance. Taken together, these data indicate a role for DCL3a in regulating plant defense pathways during both within-generation and intergenerational resistance against nematodes in rice.}},
  author       = {{Meijer, Aniko and Atighi Quchan Atigh, Mohammad and Demeestere, Kristof and De Meyer, Tim and Vandepoele, Klaas and Kyndt, Tina}},
  issn         = {{0032-0889}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{Plant Science,Genetics,Physiology}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{2071--2085}},
  title        = {{Dicer-like 3a mediates intergenerational resistance against root-knot nematodes in rice via hormone responses}},
  url          = {{http://doi.org/10.1093/plphys/kiad215}},
  volume       = {{193}},
  year         = {{2023}},
}
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