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Current challenges in designer cellulosome engineering

Babette Lamote (UGent) , Maria João de Carvalho Maurício da Fonseca (UGent) , Julie Vanderstraeten (UGent) , Kenan Meert (UGent) , Marte Elias (UGent) and Yves Briers (UGent)
(2023) APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 107(9). p.2755-2770
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Abstract
Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high-affinity cohesin-dockerin interactions. The use of DCs in the degradation of renewable biomass polymers is a promising approach for biorefineries. Indeed, DCs have shown significant hydrolytic activities due to the enhanced enzyme-substrate proximity and inter-enzyme synergies, but technical hurdles in DC engineering have hindered further progress towards industrial application. The challenge in DC engineering lies in the large diversity of possible building blocks and architectures, resulting in a multivariate and immense design space. Simultaneously, the precise DC composition affects many relevant parameters such as activity, stability, and manufacturability. Since protein engineers face a lack of high-throughput approaches to explore this vast design space, DC engineering may result in an unsatisfying outcome. This review provides a roadmap to guide researchers through the process of DC engineering. Each step, starting from concept to evaluation, is described and provided with its challenges, along with possible solutions, both for DCs that are assembled in vitro or are displayed on the yeast cell surface.
Keywords
Designer cellulosome, Lignocellulose, Biomass, Protein engineering, In, vitro assembly, Yeast cell surface display, CARBOHYDRATE-BINDING MODULES, CLOSTRIDIUM-THERMOCELLUM, DOCKERIN DOMAIN, CELL-SURFACE, SACCHAROMYCES-CEREVISIAE, COHESIN DOMAIN, YEAST SURFACE, COMPLEX, INTEGRATION, MINICELLULOSOMES

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Citation

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MLA
Lamote, Babette, et al. “Current Challenges in Designer Cellulosome Engineering.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 107, no. 9, 2023, pp. 2755–70, doi:10.1007/s00253-023-12474-8.
APA
Lamote, B., de Carvalho Maurício da Fonseca, M. J., Vanderstraeten, J., Meert, K., Elias, M., & Briers, Y. (2023). Current challenges in designer cellulosome engineering. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 107(9), 2755–2770. https://doi.org/10.1007/s00253-023-12474-8
Chicago author-date
Lamote, Babette, Maria João de Carvalho Maurício da Fonseca, Julie Vanderstraeten, Kenan Meert, Marte Elias, and Yves Briers. 2023. “Current Challenges in Designer Cellulosome Engineering.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 107 (9): 2755–70. https://doi.org/10.1007/s00253-023-12474-8.
Chicago author-date (all authors)
Lamote, Babette, Maria João de Carvalho Maurício da Fonseca, Julie Vanderstraeten, Kenan Meert, Marte Elias, and Yves Briers. 2023. “Current Challenges in Designer Cellulosome Engineering.” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 107 (9): 2755–2770. doi:10.1007/s00253-023-12474-8.
Vancouver
1.
Lamote B, de Carvalho Maurício da Fonseca MJ, Vanderstraeten J, Meert K, Elias M, Briers Y. Current challenges in designer cellulosome engineering. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 2023;107(9):2755–70.
IEEE
[1]
B. Lamote, M. J. de Carvalho Maurício da Fonseca, J. Vanderstraeten, K. Meert, M. Elias, and Y. Briers, “Current challenges in designer cellulosome engineering,” APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 107, no. 9, pp. 2755–2770, 2023.
@article{01GXXHJBVPG2N8KPA9V4HT7A72,
  abstract     = {{Designer cellulosomes (DCs) are engineered multi-enzyme complexes, comprising carbohydrate-active enzymes attached to a common backbone, the scaffoldin, via high-affinity cohesin-dockerin interactions. The use of DCs in the degradation of renewable biomass polymers is a promising approach for biorefineries. Indeed, DCs have shown significant hydrolytic activities due to the enhanced enzyme-substrate proximity and inter-enzyme synergies, but technical hurdles in DC engineering have hindered further progress towards industrial application. The challenge in DC engineering lies in the large diversity of possible building blocks and architectures, resulting in a multivariate and immense design space. Simultaneously, the precise DC composition affects many relevant parameters such as activity, stability, and manufacturability. Since protein engineers face a lack of high-throughput approaches to explore this vast design space, DC engineering may result in an unsatisfying outcome. This review provides a roadmap to guide researchers through the process of DC engineering. Each step, starting from concept to evaluation, is described and provided with its challenges, along with possible solutions, both for DCs that are assembled in vitro or are displayed on the yeast cell surface.}},
  author       = {{Lamote, Babette and de Carvalho Maurício da Fonseca, Maria João and Vanderstraeten, Julie and Meert, Kenan and Elias, Marte and Briers, Yves}},
  issn         = {{0175-7598}},
  journal      = {{APPLIED MICROBIOLOGY AND BIOTECHNOLOGY}},
  keywords     = {{Designer cellulosome,Lignocellulose,Biomass,Protein engineering,In,vitro assembly,Yeast cell surface display,CARBOHYDRATE-BINDING MODULES,CLOSTRIDIUM-THERMOCELLUM,DOCKERIN DOMAIN,CELL-SURFACE,SACCHAROMYCES-CEREVISIAE,COHESIN DOMAIN,YEAST SURFACE,COMPLEX,INTEGRATION,MINICELLULOSOMES}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{2755--2770}},
  title        = {{Current challenges in designer cellulosome engineering}},
  url          = {{http://doi.org/10.1007/s00253-023-12474-8}},
  volume       = {{107}},
  year         = {{2023}},
}
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