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Transcriptomics reveal molecular differences in equine oocytes vitrified before and after in vitro maturation

Daniel Angel Velez (UGent) , Tim Meese (UGent) , Mohamed Hedia (UGent) , Andrea Fernandez Montoro (UGent) , Tine De Coster (UGent) , Osvaldo Américo Bogado Pascottini (UGent) , Filip Van Nieuwerburgh (UGent) , Jan Govaere (UGent) , Ann Van Soom (UGent) , Krishna Pavani (UGent) , et al.
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Abstract
In the last decade, in vitro embryo production in horses has become an established clinical practice, but blastocyst rates from vitrified equine oocytes remain low. Cryopreservation impairs the oocyte developmental potential, which may be reflected in the messenger RNA (mRNA) profile. Therefore, this study aimed to compare the transcriptome profiles of metaphase II equine oocytes vitrified before and after in vitro maturation. To do so, three groups were analyzed with RNA sequencing: (1) fresh in vitro matured oocytes as a control (FR), (2) oocytes vitrified after in vitro maturation (VMAT), and (3) oocytes vitrified immature, warmed, and in vitro matured (VIM). In comparison with fresh oocytes, VIM resulted in 46 differentially expressed (DE) genes (14 upregulated and 32 downregulated), while VMAT showed 36 DE genes (18 in each category). A comparison of VIM vs. VMAT resulted in 44 DE genes (20 upregulated and 24 downregulated). Pathway analyses highlighted cytoskeleton, spindle formation, and calcium and cation ion transport and homeostasis as the main affected pathways in vitrified oocytes. The vitrification of in vitro matured oocytes presented subtle advantages in terms of the mRNA profile over the vitrification of immature oocytes. Therefore, this study provides a new perspective for understanding the impact of vitrification on equine oocytes and can be the basis for further improvements in the efficiency of equine oocyte vitrification.
Keywords
Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, Catalysis, cryopreservation, horse oocyte, RNA sequencing

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MLA
Angel Velez, Daniel, et al. “Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after in Vitro Maturation.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 8, 2023, doi:10.3390/ijms24086915.
APA
Angel Velez, D., Meese, T., Hedia, M., Fernandez Montoro, A., De Coster, T., Bogado Pascottini, O. A., … Smits, K. (2023). Transcriptomics reveal molecular differences in equine oocytes vitrified before and after in vitro maturation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(8). https://doi.org/10.3390/ijms24086915
Chicago author-date
Angel Velez, Daniel, Tim Meese, Mohamed Hedia, Andrea Fernandez Montoro, Tine De Coster, Osvaldo Américo Bogado Pascottini, Filip Van Nieuwerburgh, et al. 2023. “Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after in Vitro Maturation.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (8). https://doi.org/10.3390/ijms24086915.
Chicago author-date (all authors)
Angel Velez, Daniel, Tim Meese, Mohamed Hedia, Andrea Fernandez Montoro, Tine De Coster, Osvaldo Américo Bogado Pascottini, Filip Van Nieuwerburgh, Jan Govaere, Ann Van Soom, Krishna Pavani, and Katrien Smits. 2023. “Transcriptomics Reveal Molecular Differences in Equine Oocytes Vitrified before and after in Vitro Maturation.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (8). doi:10.3390/ijms24086915.
Vancouver
1.
Angel Velez D, Meese T, Hedia M, Fernandez Montoro A, De Coster T, Bogado Pascottini OA, et al. Transcriptomics reveal molecular differences in equine oocytes vitrified before and after in vitro maturation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2023;24(8).
IEEE
[1]
D. Angel Velez et al., “Transcriptomics reveal molecular differences in equine oocytes vitrified before and after in vitro maturation,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 8, 2023.
@article{01GXX35X6X3XG5A2EQDSPS3KPK,
  abstract     = {{In the last decade, in vitro embryo production in horses has become an established clinical practice, but blastocyst rates from vitrified equine oocytes remain low. Cryopreservation impairs the oocyte developmental potential, which may be reflected in the messenger RNA (mRNA) profile. Therefore, this study aimed to compare the transcriptome profiles of metaphase II equine oocytes vitrified before and after in vitro maturation. To do so, three groups were analyzed with RNA sequencing: (1) fresh in vitro matured oocytes as a control (FR), (2) oocytes vitrified after in vitro maturation (VMAT), and (3) oocytes vitrified immature, warmed, and in vitro matured (VIM). In comparison with fresh oocytes, VIM resulted in 46 differentially expressed (DE) genes (14 upregulated and 32 downregulated), while VMAT showed 36 DE genes (18 in each category). A comparison of VIM vs. VMAT resulted in 44 DE genes (20 upregulated and 24 downregulated). Pathway analyses highlighted cytoskeleton, spindle formation, and calcium and cation ion transport and homeostasis as the main affected pathways in vitrified oocytes. The vitrification of in vitro matured oocytes presented subtle advantages in terms of the mRNA profile over the vitrification of immature oocytes. Therefore, this study provides a new perspective for understanding the impact of vitrification on equine oocytes and can be the basis for further improvements in the efficiency of equine oocyte vitrification.}},
  articleno    = {{6915}},
  author       = {{Angel Velez, Daniel and Meese, Tim and Hedia, Mohamed and Fernandez Montoro, Andrea and De Coster, Tine and Bogado Pascottini, Osvaldo Américo and Van Nieuwerburgh, Filip and Govaere, Jan and Van Soom, Ann and Pavani, Krishna and Smits, Katrien}},
  issn         = {{1422-0067}},
  journal      = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
  keywords     = {{Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis,cryopreservation,horse oocyte,RNA sequencing}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{17}},
  title        = {{Transcriptomics reveal molecular differences in equine oocytes vitrified before and after in vitro maturation}},
  url          = {{http://doi.org/10.3390/ijms24086915}},
  volume       = {{24}},
  year         = {{2023}},
}

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