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Single-cell optical nanomotion of Candida albicans in microwells for rapid antifungal susceptibility testing

Vjera Radoničić (UGent) , Charlotte Yvanoff, Maria Ines Villalba, Bart Devreese (UGent) , Sandor Kasas and Ronnie G. Willaert
(2023) FERMENTATION-BASEL. 9(4).
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Abstract
Candida albicans is an emerging multidrug-resistant opportunistic pathogen representing an important source of invasive disease in humans and generating high healthcare costs worldwide. The development of a rapid and simple antifungal susceptibility test (AFST) could limit the spread of this disease, increase the efficiency of treatment, and lower the risk of developing resistant strains. We developed a microfluidic chip containing an array of microwells that were designed to trap the cells and perform rapid antifungal susceptibility tests using optical nanomotion detection (ONMD). Yeast cell entrapment in a microwell allows for a very rapid exchange of growth medium with the antifungal, which enables performing single-cell ONMD measurements on the same cell before and after antifungal treatment. The exposure to a low concentration of the antifungal caspofungin or flucanozole induced a significant decrease in the nanomotion signal, demonstrating the high sensitivity of this technique. We used this chip to quantify the real-time response of individual C. albicans cells to the antifungal treatment in as fast as 10 min. This simple and label-free technique could be further developed into a simple-to-use device that allows the performance of fast AFST as part of a routine hospital procedure in developed and also eventually developing world countries.
Keywords
Plant Science, Biochemistry, Genetics and Molecular Biology (miscellaneous), Food Science, cellular nanomotion, single cell, optical nanomotion detection, microfluidic chip, microwells, yeast, Candida albicans, antifungal, antifungal susceptibility testing, ANTIMICROBIAL SUSCEPTIBILITY, RESISTANCE, FLUCONAZOLE, CASPOFUNGIN, TRACKING, GENE

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Citation

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MLA
Radoničić, Vjera, et al. “Single-Cell Optical Nanomotion of Candida Albicans in Microwells for Rapid Antifungal Susceptibility Testing.” FERMENTATION-BASEL, vol. 9, no. 4, 2023, doi:10.3390/fermentation9040365.
APA
Radoničić, V., Yvanoff, C., Villalba, M. I., Devreese, B., Kasas, S., & Willaert, R. G. (2023). Single-cell optical nanomotion of Candida albicans in microwells for rapid antifungal susceptibility testing. FERMENTATION-BASEL, 9(4). https://doi.org/10.3390/fermentation9040365
Chicago author-date
Radoničić, Vjera, Charlotte Yvanoff, Maria Ines Villalba, Bart Devreese, Sandor Kasas, and Ronnie G. Willaert. 2023. “Single-Cell Optical Nanomotion of Candida Albicans in Microwells for Rapid Antifungal Susceptibility Testing.” FERMENTATION-BASEL 9 (4). https://doi.org/10.3390/fermentation9040365.
Chicago author-date (all authors)
Radoničić, Vjera, Charlotte Yvanoff, Maria Ines Villalba, Bart Devreese, Sandor Kasas, and Ronnie G. Willaert. 2023. “Single-Cell Optical Nanomotion of Candida Albicans in Microwells for Rapid Antifungal Susceptibility Testing.” FERMENTATION-BASEL 9 (4). doi:10.3390/fermentation9040365.
Vancouver
1.
Radoničić V, Yvanoff C, Villalba MI, Devreese B, Kasas S, Willaert RG. Single-cell optical nanomotion of Candida albicans in microwells for rapid antifungal susceptibility testing. FERMENTATION-BASEL. 2023;9(4).
IEEE
[1]
V. Radoničić, C. Yvanoff, M. I. Villalba, B. Devreese, S. Kasas, and R. G. Willaert, “Single-cell optical nanomotion of Candida albicans in microwells for rapid antifungal susceptibility testing,” FERMENTATION-BASEL, vol. 9, no. 4, 2023.
@article{01GXQS9TVAXE3B14Z0XVEJZWPB,
  abstract     = {{Candida albicans is an emerging multidrug-resistant opportunistic pathogen representing an important source of invasive disease in humans and generating high healthcare costs worldwide. The development of a rapid and simple antifungal susceptibility test (AFST) could limit the spread of this disease, increase the efficiency of treatment, and lower the risk of developing resistant strains. We developed a microfluidic chip containing an array of microwells that were designed to trap the cells and perform rapid antifungal susceptibility tests using optical nanomotion detection (ONMD). Yeast cell entrapment in a microwell allows for a very rapid exchange of growth medium with the antifungal, which enables performing single-cell ONMD measurements on the same cell before and after antifungal treatment. The exposure to a low concentration of the antifungal caspofungin or flucanozole induced a significant decrease in the nanomotion signal, demonstrating the high sensitivity of this technique. We used this chip to quantify the real-time response of individual C. albicans cells to the antifungal treatment in as fast as 10 min. This simple and label-free technique could be further developed into a simple-to-use device that allows the performance of fast AFST as part of a routine hospital procedure in developed and also eventually developing world countries.}},
  articleno    = {{365}},
  author       = {{Radoničić, Vjera and Yvanoff, Charlotte and Villalba, Maria Ines and Devreese, Bart and Kasas, Sandor and Willaert, Ronnie G.}},
  issn         = {{2311-5637}},
  journal      = {{FERMENTATION-BASEL}},
  keywords     = {{Plant Science,Biochemistry, Genetics and Molecular Biology (miscellaneous),Food Science,cellular nanomotion,single cell,optical nanomotion detection,microfluidic chip,microwells,yeast,Candida albicans,antifungal,antifungal susceptibility testing,ANTIMICROBIAL SUSCEPTIBILITY,RESISTANCE,FLUCONAZOLE,CASPOFUNGIN,TRACKING,GENE}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{27}},
  title        = {{Single-cell optical nanomotion of Candida albicans in microwells for rapid antifungal susceptibility testing}},
  url          = {{http://doi.org/10.3390/fermentation9040365}},
  volume       = {{9}},
  year         = {{2023}},
}
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