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Investigating the effect of diluents and fat globules on the size measurement of casein micelles by dynamic light scattering

Teng Wang (UGent) , Hao Li (UGent) , Quenten Denon (UGent) , Chak Ming To (UGent) and Paul Van der Meeren (UGent)
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Abstract
The hydrodynamic size of casein micelles was examined using dynamic light scattering (DLS). Four types of diluents [ultrafiltration (UF) permeate, lactose-free simulated milk ultrafiltrate (SMUF), calcium imidazole buffer, and deionised water] were compared in their effect on the stability of casein micelles. Results of dilution series and kinetic size measurements revealed that both UF permeate and lactose-free SMUF enable size measurements that are hardly affected by the required dilution step, whereas a calcium imidazole buffer containing 10 mM CaCl2 only could provide accurate sizing results within a short time after dilution. The contribution of residual fat globules to the particle size distribution was negligible in skim milk. For semi-skimmed milk, the size of the casein micelles could be estimated by subtracting the contribution of the fat droplets (obtained upon dilution in a casein dissolving solution) from the raw DLS data (as obtained upon dilution in milk ultrafiltrate). (c) 2023 Elsevier Ltd. All rights reserved.
Keywords
COLLOIDAL CALCIUM-PHOSPHATE, SKIM MILK, PH, DISSOCIATION, TEMPERATURE, MODEL

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MLA
Wang, Teng, et al. “Investigating the Effect of Diluents and Fat Globules on the Size Measurement of Casein Micelles by Dynamic Light Scattering.” INTERNATIONAL DAIRY JOURNAL, vol. 142, 2023, doi:10.1016/j.idairyj.2023.105640.
APA
Wang, T., Li, H., Denon, Q., To, C. M., & Van der Meeren, P. (2023). Investigating the effect of diluents and fat globules on the size measurement of casein micelles by dynamic light scattering. INTERNATIONAL DAIRY JOURNAL, 142. https://doi.org/10.1016/j.idairyj.2023.105640
Chicago author-date
Wang, Teng, Hao Li, Quenten Denon, Chak Ming To, and Paul Van der Meeren. 2023. “Investigating the Effect of Diluents and Fat Globules on the Size Measurement of Casein Micelles by Dynamic Light Scattering.” INTERNATIONAL DAIRY JOURNAL 142. https://doi.org/10.1016/j.idairyj.2023.105640.
Chicago author-date (all authors)
Wang, Teng, Hao Li, Quenten Denon, Chak Ming To, and Paul Van der Meeren. 2023. “Investigating the Effect of Diluents and Fat Globules on the Size Measurement of Casein Micelles by Dynamic Light Scattering.” INTERNATIONAL DAIRY JOURNAL 142. doi:10.1016/j.idairyj.2023.105640.
Vancouver
1.
Wang T, Li H, Denon Q, To CM, Van der Meeren P. Investigating the effect of diluents and fat globules on the size measurement of casein micelles by dynamic light scattering. INTERNATIONAL DAIRY JOURNAL. 2023;142.
IEEE
[1]
T. Wang, H. Li, Q. Denon, C. M. To, and P. Van der Meeren, “Investigating the effect of diluents and fat globules on the size measurement of casein micelles by dynamic light scattering,” INTERNATIONAL DAIRY JOURNAL, vol. 142, 2023.
@article{01GX6W99YFSW5JSQ1QW55W9WRE,
  abstract     = {{The hydrodynamic size of casein micelles was examined using dynamic light scattering (DLS). Four types of diluents [ultrafiltration (UF) permeate, lactose-free simulated milk ultrafiltrate (SMUF), calcium imidazole buffer, and deionised water] were compared in their effect on the stability of casein micelles. Results of dilution series and kinetic size measurements revealed that both UF permeate and lactose-free SMUF enable size measurements that are hardly affected by the required dilution step, whereas a calcium imidazole buffer containing 10 mM CaCl2 only could provide accurate sizing results within a short time after dilution. The contribution of residual fat globules to the particle size distribution was negligible in skim milk. For semi-skimmed milk, the size of the casein micelles could be estimated by subtracting the contribution of the fat droplets (obtained upon dilution in a casein dissolving solution) from the raw DLS data (as obtained upon dilution in milk ultrafiltrate). (c) 2023 Elsevier Ltd. All rights reserved.}},
  articleno    = {{105640}},
  author       = {{Wang, Teng and Li, Hao and Denon, Quenten and To, Chak Ming and Van der Meeren, Paul}},
  issn         = {{0958-6946}},
  journal      = {{INTERNATIONAL DAIRY JOURNAL}},
  keywords     = {{COLLOIDAL CALCIUM-PHOSPHATE,SKIM MILK,PH,DISSOCIATION,TEMPERATURE,MODEL}},
  language     = {{eng}},
  pages        = {{8}},
  title        = {{Investigating the effect of diluents and fat globules on the size measurement of casein micelles by dynamic light scattering}},
  url          = {{http://doi.org/10.1016/j.idairyj.2023.105640}},
  volume       = {{142}},
  year         = {{2023}},
}

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