Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 13.45 MB
Add to list
  1. Search results
  2. Reflective multi-immersion microscope...

Reflective multi-immersion microscope objectives inspired by the Schmidt telescope

Fabian F. Voigt, Anna Maria Reuss, Thomas Naert (UGent) , Sven Hildebrand, Martina Schaettin, Adriana L. Hotz, Lachlan Whitehead, Armin Bahl, Stephan C. F. Neuhauss, Alard Roebroeck, et al.
(2024) NATURE BIOTECHNOLOGY. 42(1). p.65-71
Author
Organization
Abstract
Imaging large, cleared samples requires microscope objectives that combine a large field of view (FOV) with a long working distance (WD) and a high numerical aperture (NA). Ideally, such objectives should be compatible with a wide range of immersion media, which is challenging to achieve with conventional lens-based objective designs. Here we introduce the multi-immersion ‘Schmidt objective’ consisting of a spherical mirror and an aspherical correction plate as a solution to this problem. We demonstrate that a multi-photon variant of the Schmidt objective is compatible with all homogeneous immersion media and achieves an NA of 1.08 at a refractive index of 1.56, 1.1-mm FOV and 11-mm WD. We highlight its versatility by imaging cleared samples in various media ranging from air and water to benzyl alcohol/benzyl benzoate, dibenzyl ether and ethyl cinnamate and by imaging of neuronal activity in larval zebrafish in vivo. In principle, the concept can be extended to any imaging modality, including wide-field, confocal and light-sheet microscopy.
Keywords
Biomedical Engineering, Molecular Medicine, Applied Microbiology and Biotechnology, Bioengineering, Biotechnology

Downloads

  • Download
    Naert2.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 13.45 MB

Citation

Please use this url to cite or link to this publication:

MLA
Voigt, Fabian F., et al. “Reflective Multi-Immersion Microscope Objectives Inspired by the Schmidt Telescope.” NATURE BIOTECHNOLOGY, vol. 42, no. 1, 2024, pp. 65–71, doi:10.1038/s41587-023-01717-8.
APA
Voigt, F. F., Reuss, A. M., Naert, T., Hildebrand, S., Schaettin, M., Hotz, A. L., … Helmchen, F. (2024). Reflective multi-immersion microscope objectives inspired by the Schmidt telescope. NATURE BIOTECHNOLOGY, 42(1), 65–71. https://doi.org/10.1038/s41587-023-01717-8
Chicago author-date
Voigt, Fabian F., Anna Maria Reuss, Thomas Naert, Sven Hildebrand, Martina Schaettin, Adriana L. Hotz, Lachlan Whitehead, et al. 2024. “Reflective Multi-Immersion Microscope Objectives Inspired by the Schmidt Telescope.” NATURE BIOTECHNOLOGY 42 (1): 65–71. https://doi.org/10.1038/s41587-023-01717-8.
Chicago author-date (all authors)
Voigt, Fabian F., Anna Maria Reuss, Thomas Naert, Sven Hildebrand, Martina Schaettin, Adriana L. Hotz, Lachlan Whitehead, Armin Bahl, Stephan C. F. Neuhauss, Alard Roebroeck, Esther T. Stoeckli, Soeren S. Lienkamp, Adriano Aguzzi, and Fritjof Helmchen. 2024. “Reflective Multi-Immersion Microscope Objectives Inspired by the Schmidt Telescope.” NATURE BIOTECHNOLOGY 42 (1): 65–71. doi:10.1038/s41587-023-01717-8.
Vancouver
1.
Voigt FF, Reuss AM, Naert T, Hildebrand S, Schaettin M, Hotz AL, et al. Reflective multi-immersion microscope objectives inspired by the Schmidt telescope. NATURE BIOTECHNOLOGY. 2024;42(1):65–71.
IEEE
[1]
F. F. Voigt et al., “Reflective multi-immersion microscope objectives inspired by the Schmidt telescope,” NATURE BIOTECHNOLOGY, vol. 42, no. 1, pp. 65–71, 2024.
@article{01GX3TTG70VBC71GYYCTHV884P,
  abstract     = {{Imaging large, cleared samples requires microscope objectives that combine a large field of view (FOV) with a long working distance (WD) and a high numerical aperture (NA). Ideally, such objectives should be compatible with a wide range of immersion media, which is challenging to achieve with conventional lens-based objective designs. Here we introduce the multi-immersion ‘Schmidt objective’ consisting of a spherical mirror and an aspherical correction plate as a solution to this problem. We demonstrate that a multi-photon variant of the Schmidt objective is compatible with all homogeneous immersion media and achieves an NA of 1.08 at a refractive index of 1.56, 1.1-mm FOV and 11-mm WD. We highlight its versatility by imaging cleared samples in various media ranging from air and water to benzyl alcohol/benzyl benzoate, dibenzyl ether and ethyl cinnamate and by imaging of neuronal activity in larval zebrafish in vivo. In principle, the concept can be extended to any imaging modality, including wide-field, confocal and light-sheet microscopy.}},
  author       = {{Voigt, Fabian F. and Reuss, Anna Maria and Naert, Thomas and Hildebrand, Sven and Schaettin, Martina and Hotz, Adriana L. and Whitehead, Lachlan and Bahl, Armin and Neuhauss, Stephan C. F. and Roebroeck, Alard and Stoeckli, Esther T. and Lienkamp, Soeren S. and Aguzzi, Adriano and Helmchen, Fritjof}},
  issn         = {{1087-0156}},
  journal      = {{NATURE BIOTECHNOLOGY}},
  keywords     = {{Biomedical Engineering,Molecular Medicine,Applied Microbiology and Biotechnology,Bioengineering,Biotechnology}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{65--71}},
  title        = {{Reflective multi-immersion microscope objectives inspired by the Schmidt telescope}},
  url          = {{http://doi.org/10.1038/s41587-023-01717-8}},
  volume       = {{42}},
  year         = {{2024}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: