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Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root

(2023) SCIENCE. 379(6639).
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Abstract
Brassinosteroids are plant steroid hormones that regulate diverse processes, such as cell division and cell elongation, through gene regulatory networks that vary in space and time. By using time series single-cell RNA sequencing to profile brassinosteroid-responsive gene expression specific to different cell types and developmental stages of the Arabidopsis root, we identified the elongating cortex as a site where brassinosteroids trigger a shift from proliferation to elongation associated with increased expression of cell wall–related genes. Our analysis revealed HOMEOBOX FROM ARABIDOPSIS THALIANA 7 (HAT7) and GT-2-LIKE 1 (GTL1) as brassinosteroid-responsive transcription factors that regulate cortex cell elongation. These results establish the cortex as a site of brassinosteroid-mediated growth and unveil a brassinosteroid signaling network regulating the transition from proliferation to elongation, which illuminates aspects of spatiotemporal hormone responses.
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Multidisciplinary

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MLA
Nolan, Trevor M., et al. “Brassinosteroid Gene Regulatory Networks at Cellular Resolution in the Arabidopsis Root.” SCIENCE, vol. 379, no. 6639, 2023, doi:10.1126/science.adf4721.
APA
Nolan, T. M., Vukasinovic, N., Hsu, C.-W., Zhang, J., Vanhoutte, I., Shahan, R., … Benfey, P. N. (2023). Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root. SCIENCE, 379(6639). https://doi.org/10.1126/science.adf4721
Chicago author-date
Nolan, Trevor M., Nemanja Vukasinovic, Che-Wei Hsu, Jingyuan Zhang, Isabelle Vanhoutte, Rachel Shahan, Isaiah W. Taylor, et al. 2023. “Brassinosteroid Gene Regulatory Networks at Cellular Resolution in the Arabidopsis Root.” SCIENCE 379 (6639). https://doi.org/10.1126/science.adf4721.
Chicago author-date (all authors)
Nolan, Trevor M., Nemanja Vukasinovic, Che-Wei Hsu, Jingyuan Zhang, Isabelle Vanhoutte, Rachel Shahan, Isaiah W. Taylor, Laura Greenstreet, Matthieu Heitz, Anton Afanassiev, Ping Wang, Pablo Szekely, Aiden Brosnan, Yanhai Yin, Geoffrey Schiebinger, Uwe Ohler, Eugenia Russinova, and Philip N. Benfey. 2023. “Brassinosteroid Gene Regulatory Networks at Cellular Resolution in the Arabidopsis Root.” SCIENCE 379 (6639). doi:10.1126/science.adf4721.
Vancouver
1.
Nolan TM, Vukasinovic N, Hsu C-W, Zhang J, Vanhoutte I, Shahan R, et al. Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root. SCIENCE. 2023;379(6639).
IEEE
[1]
T. M. Nolan et al., “Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root,” SCIENCE, vol. 379, no. 6639, 2023.
@article{01GX3832S6RHTHB2KPR99Z4JYS,
  abstract     = {{Brassinosteroids are plant steroid hormones that regulate diverse processes, such as cell division and cell elongation, through gene regulatory networks that vary in space and time. By using time series single-cell RNA sequencing to profile brassinosteroid-responsive gene expression specific to different cell types and developmental stages of the Arabidopsis root, we identified the elongating cortex as a site where brassinosteroids trigger a shift from proliferation to elongation associated with increased expression of cell wall–related genes. Our analysis revealed HOMEOBOX FROM ARABIDOPSIS THALIANA 7 (HAT7) and GT-2-LIKE 1 (GTL1) as brassinosteroid-responsive transcription factors that regulate cortex cell elongation. These results establish the cortex as a site of brassinosteroid-mediated growth and unveil a brassinosteroid signaling network regulating the transition from proliferation to elongation, which illuminates aspects of spatiotemporal hormone responses.}},
  articleno    = {{eadf4721}},
  author       = {{Nolan, Trevor M. and Vukasinovic, Nemanja and Hsu, Che-Wei and Zhang, Jingyuan and Vanhoutte, Isabelle and Shahan, Rachel and Taylor, Isaiah W. and Greenstreet, Laura and Heitz, Matthieu and Afanassiev, Anton and Wang, Ping and Szekely, Pablo and Brosnan, Aiden and Yin, Yanhai and Schiebinger, Geoffrey and Ohler, Uwe and Russinova, Eugenia and Benfey, Philip N.}},
  issn         = {{0036-8075}},
  journal      = {{SCIENCE}},
  keywords     = {{Multidisciplinary}},
  language     = {{eng}},
  number       = {{6639}},
  pages        = {{13}},
  title        = {{Brassinosteroid gene regulatory networks at cellular resolution in the Arabidopsis root}},
  url          = {{http://doi.org/10.1126/science.adf4721}},
  volume       = {{379}},
  year         = {{2023}},
}

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