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A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis

(2023) NATURE PLANTS. 9(6). p.926-937
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Abstract
Plant specialized metabolite biosynthesis is strictly regulated in time and space. The authors describe a robust and redundant transcriptional network that steers cell-specific and jasmonate-inducible triterpene biosynthesis in Arabidopsis root tips. Plant specialized metabolites modulate developmental and ecological functions and comprise many therapeutic and other high-value compounds. However, the mechanisms determining their cell-specific expression remain unknown. Here we describe the transcriptional regulatory network that underlies cell-specific biosynthesis of triterpenes in Arabidopsis thaliana root tips. Expression of thalianol and marneral biosynthesis pathway genes depends on the phytohormone jasmonate and is limited to outer tissues. We show that this is promoted by the activity of redundant bHLH-type transcription factors from two distinct clades and coactivated by homeodomain factors. Conversely, the DOF-type transcription factor DAG1 and other regulators prevent expression of the triterpene pathway genes in inner tissues. We thus show how precise expression of triterpene biosynthesis genes is determined by a robust network of transactivators, coactivators and counteracting repressors.
Keywords
JASMONATE-REGULATED DEFENSE, DOF PROTEIN DAG1, GENE CLUSTERS, BIOSYNTHESIS, EXPRESSION, MYC3, GERMINATION, REPRESSION, GLABRA2, TARGETS

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Citation

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MLA
Nguyen, Trang Hieu, et al. “A Redundant Transcription Factor Network Steers Spatiotemporal Arabidopsis Triterpene Synthesis.” NATURE PLANTS, vol. 9, no. 6, 2023, pp. 926–37, doi:10.1038/s41477-023-01419-8.
APA
Nguyen, T. H., Thiers, L., Van Moerkercke, A., Bai, Y., Fernandez Calvo, P., Minne, M., … Goossens, A. (2023). A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis. NATURE PLANTS, 9(6), 926–937. https://doi.org/10.1038/s41477-023-01419-8
Chicago author-date
Nguyen, Trang Hieu, Louis Thiers, Alex Van Moerkercke, Yuechen Bai, Patricia Fernandez Calvo, Max Minne, Thomas Depuydt, et al. 2023. “A Redundant Transcription Factor Network Steers Spatiotemporal Arabidopsis Triterpene Synthesis.” NATURE PLANTS 9 (6): 926–37. https://doi.org/10.1038/s41477-023-01419-8.
Chicago author-date (all authors)
Nguyen, Trang Hieu, Louis Thiers, Alex Van Moerkercke, Yuechen Bai, Patricia Fernandez Calvo, Max Minne, Thomas Depuydt, Maite Colinas Martinez, Kevin Verstaen, Gert Van Isterdael, Hans-Wilhelm Nützmann, Anne Osbourn, Yvan Saeys, Bert De Rybel, Klaas Vandepoele, Andrés Ritter Traub, and Alain Goossens. 2023. “A Redundant Transcription Factor Network Steers Spatiotemporal Arabidopsis Triterpene Synthesis.” NATURE PLANTS 9 (6): 926–937. doi:10.1038/s41477-023-01419-8.
Vancouver
1.
Nguyen TH, Thiers L, Van Moerkercke A, Bai Y, Fernandez Calvo P, Minne M, et al. A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis. NATURE PLANTS. 2023;9(6):926–37.
IEEE
[1]
T. H. Nguyen et al., “A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis,” NATURE PLANTS, vol. 9, no. 6, pp. 926–937, 2023.
@article{01GWRM6W6DWBSDMHQY6GSBFWKQ,
  abstract     = {{Plant specialized metabolite biosynthesis is strictly regulated in time and space. The authors describe a robust and redundant transcriptional network that steers cell-specific and jasmonate-inducible triterpene biosynthesis in Arabidopsis root tips.

Plant specialized metabolites modulate developmental and ecological functions and comprise many therapeutic and other high-value compounds. However, the mechanisms determining their cell-specific expression remain unknown. Here we describe the transcriptional regulatory network that underlies cell-specific biosynthesis of triterpenes in Arabidopsis thaliana root tips. Expression of thalianol and marneral biosynthesis pathway genes depends on the phytohormone jasmonate and is limited to outer tissues. We show that this is promoted by the activity of redundant bHLH-type transcription factors from two distinct clades and coactivated by homeodomain factors. Conversely, the DOF-type transcription factor DAG1 and other regulators prevent expression of the triterpene pathway genes in inner tissues. We thus show how precise expression of triterpene biosynthesis genes is determined by a robust network of transactivators, coactivators and counteracting repressors.}},
  author       = {{Nguyen, Trang Hieu and Thiers, Louis and Van Moerkercke, Alex and Bai, Yuechen and Fernandez Calvo, Patricia and Minne, Max and Depuydt, Thomas and Colinas Martinez, Maite and Verstaen, Kevin and Van Isterdael, Gert and Nützmann, Hans-Wilhelm and Osbourn, Anne and Saeys, Yvan and De Rybel, Bert and Vandepoele, Klaas and Ritter Traub, Andrés and Goossens, Alain}},
  issn         = {{2055-026X}},
  journal      = {{NATURE PLANTS}},
  keywords     = {{JASMONATE-REGULATED DEFENSE,DOF PROTEIN DAG1,GENE CLUSTERS,BIOSYNTHESIS,EXPRESSION,MYC3,GERMINATION,REPRESSION,GLABRA2,TARGETS}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{926--937}},
  title        = {{A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis}},
  url          = {{http://doi.org/10.1038/s41477-023-01419-8}},
  volume       = {{9}},
  year         = {{2023}},
}

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