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Nature-inspired dual purpose strategy toward cell-adhesive PCL networks : C(-linker-)RGD incorporation via thiol-ene crosslinking

(2023) BIOMACROMOLECULES. 24(4). p.1638-1647
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Abstract
In an attempt to mimic nature's ability to adhere cells, PCL is often coated with nature-derived polymers or its surface is functionalized with a cell-binding motif. However, said surface modifications are limited to the material's surface, include multiple steps, and are mediated by harsh conditions. Here, we introduce a single-step strategy toward cell-adhesive polymer networks where thiol-ene chemistry serves a dual purpose. First, alkene-functionalized PCL is crosslinked by means of a multifunctional thiol. Second, by means of a cysteine coupling site, the cell-binding motif C(-linker-)RGD is covalently bound throughout the PCL networks during crosslinking. Moreover, the influence of various linkers (type and length), between the cysteine coupling site and the cell-binding motif RGD, is investigated and the functionalization is assessed by means of static contact angle measurements and X-ray photoelectron spectroscopy. Finally, successful introduction of cell adhesiveness is illustrated for the networks by seeding fibroblasts onto the functionalized PCL networks.

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MLA
Thijssen, Quinten, et al. “Nature-Inspired Dual Purpose Strategy toward Cell-Adhesive PCL Networks : C(-Linker-)RGD Incorporation via Thiol-Ene Crosslinking.” BIOMACROMOLECULES, vol. 24, no. 4, 2023, pp. 1638–47, doi:10.1021/acs.biomac.2c01389.
APA
Thijssen, Q., Parmentier, L., Van holsbeeck, K., Ballet, S., & Van Vlierberghe, S. (2023). Nature-inspired dual purpose strategy toward cell-adhesive PCL networks : C(-linker-)RGD incorporation via thiol-ene crosslinking. BIOMACROMOLECULES, 24(4), 1638–1647. https://doi.org/10.1021/acs.biomac.2c01389
Chicago author-date
Thijssen, Quinten, Laurens Parmentier, Kevin Van holsbeeck, Steven Ballet, and Sandra Van Vlierberghe. 2023. “Nature-Inspired Dual Purpose Strategy toward Cell-Adhesive PCL Networks : C(-Linker-)RGD Incorporation via Thiol-Ene Crosslinking.” BIOMACROMOLECULES 24 (4): 1638–47. https://doi.org/10.1021/acs.biomac.2c01389.
Chicago author-date (all authors)
Thijssen, Quinten, Laurens Parmentier, Kevin Van holsbeeck, Steven Ballet, and Sandra Van Vlierberghe. 2023. “Nature-Inspired Dual Purpose Strategy toward Cell-Adhesive PCL Networks : C(-Linker-)RGD Incorporation via Thiol-Ene Crosslinking.” BIOMACROMOLECULES 24 (4): 1638–1647. doi:10.1021/acs.biomac.2c01389.
Vancouver
1.
Thijssen Q, Parmentier L, Van holsbeeck K, Ballet S, Van Vlierberghe S. Nature-inspired dual purpose strategy toward cell-adhesive PCL networks : C(-linker-)RGD incorporation via thiol-ene crosslinking. BIOMACROMOLECULES. 2023;24(4):1638–47.
IEEE
[1]
Q. Thijssen, L. Parmentier, K. Van holsbeeck, S. Ballet, and S. Van Vlierberghe, “Nature-inspired dual purpose strategy toward cell-adhesive PCL networks : C(-linker-)RGD incorporation via thiol-ene crosslinking,” BIOMACROMOLECULES, vol. 24, no. 4, pp. 1638–1647, 2023.
@article{01GW701PV0AD951A3FGCEKVCM8,
  abstract     = {{In an attempt to mimic nature's ability to adhere cells, PCL is often coated with nature-derived polymers or its surface is functionalized with a cell-binding motif. However, said surface modifications are limited to the material's surface, include multiple steps, and are mediated by harsh conditions. Here, we introduce a single-step strategy toward cell-adhesive polymer networks where thiol-ene chemistry serves a dual purpose. First, alkene-functionalized PCL is crosslinked by means of a multifunctional thiol. Second, by means of a cysteine coupling site, the cell-binding motif C(-linker-)RGD is covalently bound throughout the PCL networks during crosslinking. Moreover, the influence of various linkers (type and length), between the cysteine coupling site and the cell-binding motif RGD, is investigated and the functionalization is assessed by means of static contact angle measurements and X-ray photoelectron spectroscopy. Finally, successful introduction of cell adhesiveness is illustrated for the networks by seeding fibroblasts onto the functionalized PCL networks.}},
  author       = {{Thijssen, Quinten and Parmentier, Laurens and Van holsbeeck, Kevin and Ballet, Steven and Van Vlierberghe, Sandra}},
  issn         = {{1525-7797}},
  journal      = {{BIOMACROMOLECULES}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{1638--1647}},
  title        = {{Nature-inspired dual purpose strategy toward cell-adhesive PCL networks : C(-linker-)RGD incorporation via thiol-ene crosslinking}},
  url          = {{http://doi.org/10.1021/acs.biomac.2c01389}},
  volume       = {{24}},
  year         = {{2023}},
}

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