@article{01GW2ZX9X7P9Y1S0XVHPX4XKX5,
  abstract     = {{The sensitive and specific detection of peptides at low levels in biofluids is critical to increase the lab-to-human translation of peptidomic research. An interesting group of peptides with increasing evidence for involvement in human diseases are quorum sensing peptides. To obtain more reliable conclusions on peptide measurands in biofluids, a selection of often neglected parts of the analytical process using LC-MS were investigated, with novel approaches recommended for each part. Quorum sensing peptides were used as the main model-peptides.

The peptidomic parts investigated and discussed here are:

1.
Sample collection: obtaining and storing biofluids such as plasma/serum, feces or saliva from mice and humans up to the laboratory handling (sample preparation) should ensure no degradation/metabolization of the peptide measurand (yielding false negatives) or of proteins (yielding false positives).


2.
Sample preparation: to remove interfering compounds as well as to release peptides from the adsorbing matrix-components and to preconcentrate them; this is a crucial step which should assure analytical stability and adsorption-minimization.


3.
Chromatography: not only the separation power and orthogonality of the complementary systems is critical, but a careful characterisation of the gradient-system, such as the starting conditions, need to be addressed as well because of worsened detection limits (yielding false negatives) or carry-over (false positives).


4.
MS detection: operational parameters such as duty cycle characteristics applied are critical in obtaining low-level, reliable results.


Our work addresses aQbD-approached solutions to these challenges, encompassing sample stabilization measures, a suitable peptide anti-adsorption tool, judicious choice of injection solvent versus gradient system and optimal duty cycle parameters. Our recommendations will improve the peptidomics bio-analytics of not only quorum sensing peptides, but can also be of value for other measurands at low concentrations.}},
  articleno    = {{115348}},
  author       = {{Descamps, Amélie and Van der borght, Kevin and De Spiegeleer, Anton and Wynendaele, Evelien and De Spiegeleer, Bart}},
  issn         = {{0731-7085}},
  journal      = {{JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS}},
  keywords     = {{LC-MS,Quorum sensing peptides,aQbD,Bio-analysis,Microbiome,Peptidomics,Quorum Sensing Peptides,AQbD,PRESSURE LIQUID-CHROMATOGRAPHY,MASS-SPECTROMETRY,ADSORPTION,PEPTIDES}},
  language     = {{eng}},
  pages        = {{7}},
  title        = {{Peptidomics : LC-MS operational parameters do matter}},
  url          = {{http://doi.org/10.1016/j.jpba.2023.115348}},
  volume       = {{229}},
  year         = {{2023}},
}

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