Antitranspirants putrescine and abscisic acid improve acclimatization of micropropagated Spathiphyllum 'Lima' regel
- Author
- Emmy Dhooghe (UGent) , Peter Lootens, Christof Van Poucke, Ellen De Keyser and Johan Van Huylenbroeck
- Organization
- Abstract
- A crucial phase for successful plant micropropagation is the transfer from lab to greenhouse. This acclimatiza-tion coincides often with plant losses. The possibility to improve acclimatization of Spathiphyllum plantlets were evaluated by the application of antitranspirants putrescine and abscisic acid (ABA) 3 days before transfer to ex vitro conditions. Different concentrations of putrescine (0, 0.882, 2.645, 4.408, and 8.815 g l-1) and ABA (0, 0.5, 5, and 50 mg l-1) were screened. For putrescine only 2.645 g l-1 resulted in higher acclimatization rates. Overall ABA improved acclimatization compared to the control treatment. No significant difference in the relative water content (RWC) was observed between control and the antitranspirants treatments. The impact at the physiological level of the best treatments (putrescine 2.645 g l-1 and ABA 0.5 mg l-1) were studied more into depth. Transpiration rate and stomatal conductance revealed that the most stressful days are the first days of the acclimatization phase. Antitranspirants had little significant impact on photosynthetic parameters, nev-ertheless ABA-treated plantlets showed less stress according to chlorophyll a fluorescence parameters. The endogenous ABA content increased during the first day of the acclimatization process. This research shows that antitranspirants can enhance acclimatization but the causality of their effectiveness is not unambiguous.
- Keywords
- abiotic stress, Araceae, plant tissue culture, transpiration, EX-VITRO ACCLIMATIZATION, CARBON METABOLISM, ORNAMENTAL PLANTS, IN-VITRO, GROWTH, PHOTOSYNTHESIS, STRESS, LIGHT, ABA, CO2
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GV5AGJHVP9QBWDTN23R698BG
- MLA
- Dhooghe, Emmy, et al. “Antitranspirants Putrescine and Abscisic Acid Improve Acclimatization of Micropropagated Spathiphyllum ‘Lima’ Regel.” PROPAGATION OF ORNAMENTAL PLANTS, vol. 21, no. 3, 2021, pp. 67–77.
- APA
- Dhooghe, E., Lootens, P., Van Poucke, C., De Keyser, E., & Van Huylenbroeck, J. (2021). Antitranspirants putrescine and abscisic acid improve acclimatization of micropropagated Spathiphyllum “Lima” regel. PROPAGATION OF ORNAMENTAL PLANTS, 21(3), 67–77.
- Chicago author-date
- Dhooghe, Emmy, Peter Lootens, Christof Van Poucke, Ellen De Keyser, and Johan Van Huylenbroeck. 2021. “Antitranspirants Putrescine and Abscisic Acid Improve Acclimatization of Micropropagated Spathiphyllum ‘Lima’ Regel.” PROPAGATION OF ORNAMENTAL PLANTS 21 (3): 67–77.
- Chicago author-date (all authors)
- Dhooghe, Emmy, Peter Lootens, Christof Van Poucke, Ellen De Keyser, and Johan Van Huylenbroeck. 2021. “Antitranspirants Putrescine and Abscisic Acid Improve Acclimatization of Micropropagated Spathiphyllum ‘Lima’ Regel.” PROPAGATION OF ORNAMENTAL PLANTS 21 (3): 67–77.
- Vancouver
- 1.Dhooghe E, Lootens P, Van Poucke C, De Keyser E, Van Huylenbroeck J. Antitranspirants putrescine and abscisic acid improve acclimatization of micropropagated Spathiphyllum “Lima” regel. PROPAGATION OF ORNAMENTAL PLANTS. 2021;21(3):67–77.
- IEEE
- [1]E. Dhooghe, P. Lootens, C. Van Poucke, E. De Keyser, and J. Van Huylenbroeck, “Antitranspirants putrescine and abscisic acid improve acclimatization of micropropagated Spathiphyllum ‘Lima’ regel,” PROPAGATION OF ORNAMENTAL PLANTS, vol. 21, no. 3, pp. 67–77, 2021.
@article{01GV5AGJHVP9QBWDTN23R698BG,
abstract = {{A crucial phase for successful plant micropropagation is the transfer from lab to greenhouse. This acclimatiza-tion coincides often with plant losses. The possibility to improve acclimatization of Spathiphyllum plantlets were evaluated by the application of antitranspirants putrescine and abscisic acid (ABA) 3 days before transfer to ex vitro conditions. Different concentrations of putrescine (0, 0.882, 2.645, 4.408, and 8.815 g l-1) and ABA (0, 0.5, 5, and 50 mg l-1) were screened. For putrescine only 2.645 g l-1 resulted in higher acclimatization rates. Overall ABA improved acclimatization compared to the control treatment. No significant difference in the relative water content (RWC) was observed between control and the antitranspirants treatments. The impact at the physiological level of the best treatments (putrescine 2.645 g l-1 and ABA 0.5 mg l-1) were studied more into depth. Transpiration rate and stomatal conductance revealed that the most stressful days are the first days of the acclimatization phase. Antitranspirants had little significant impact on photosynthetic parameters, nev-ertheless ABA-treated plantlets showed less stress according to chlorophyll a fluorescence parameters. The endogenous ABA content increased during the first day of the acclimatization process. This research shows that antitranspirants can enhance acclimatization but the causality of their effectiveness is not unambiguous.}},
author = {{Dhooghe, Emmy and Lootens, Peter and Van Poucke, Christof and De Keyser, Ellen and Van Huylenbroeck, Johan}},
issn = {{1311-9109}},
journal = {{PROPAGATION OF ORNAMENTAL PLANTS}},
keywords = {{abiotic stress,Araceae,plant tissue culture,transpiration,EX-VITRO ACCLIMATIZATION,CARBON METABOLISM,ORNAMENTAL PLANTS,IN-VITRO,GROWTH,PHOTOSYNTHESIS,STRESS,LIGHT,ABA,CO2}},
language = {{eng}},
number = {{3}},
pages = {{67--77}},
title = {{Antitranspirants putrescine and abscisic acid improve acclimatization of micropropagated Spathiphyllum 'Lima' regel}},
volume = {{21}},
year = {{2021}},
}