Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
2 files | 4.63 MB
Add to list
  1. Search results
  2. BRASSINOSTEROID INSENSITIVE1 internal...

BRASSINOSTEROID INSENSITIVE1 internalization can occur independent of ligand binding

Lucas Alves Neubus Claus, Derui Liu (UGent) , Ulrich Hohmann, Nemanja Vukasinovic (UGent) , Roman Pleskot (UGent) , Jing Liu, Alexei Schiffner, Yvon Jaillais, Guang Wu, Sebastian Wolf, et al.
(2023) PLANT PHYSIOLOGY. 192(1). p.65-76
Author
Organization
Project
Abstract
The brassinosteroid (BR) hormone and its plasma membrane receptor BR INSENSITIVE1 (BRI1) is one of the best-studied receptor-ligand pairs for understanding the interplay between receptor endocytosis and signaling in plants. BR signaling is mainly determined by the plasma membrane pool of BRI1, whereas BRI1 endocytosis ensures signal attenuation. Since BRs are ubiquitously distributed in the plant, the tools available to study BRI1 function without interference from endogenous BRs are limited. Here, we designed a BR-binding-deficient Arabidopsis (Arabidopsis thaliana) mutant based on protein sequence-structure analysis and homology modeling of members of BRI1 family. This tool allowed us to re-examine the BRI1 endocytosis and signal attenuation model. We showed that despite impaired phosphorylation and ubiquitination, BR-binding-deficient BRI1 internalizes similarly to the wild-type form. Our data indicate that BRI1 internalization relies on different endocytic machinery. In addition, the BR-binding-deficient mutant provides opportunities to study non-canonical ligand-independent BRI1 functions.
Keywords
BRI1, brassinosteroids, endocytosis, ligand-binding, non-canonical, EXTRACELLULAR DOMAIN, RECEPTOR, ENDOCYTOSIS, KINASE, PERCEPTION, TRAFFICKING, DEGRADATION, PROTEINS

Downloads

  • Download
    Alves Neubus Claus MS and SI.pdf
    • full text (Accepted manuscript)
    • |
    • open access
    • |
    • PDF
    • |
    • 3.28 MB
  • Download
    (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.35 MB

Citation

Please use this url to cite or link to this publication:

MLA
Alves Neubus Claus, Lucas, et al. “BRASSINOSTEROID INSENSITIVE1 Internalization Can Occur Independent of Ligand Binding.” PLANT PHYSIOLOGY, vol. 192, no. 1, 2023, pp. 65–76, doi:10.1093/plphys/kiad005.
APA
Alves Neubus Claus, L., Liu, D., Hohmann, U., Vukasinovic, N., Pleskot, R., Liu, J., … Russinova, E. (2023). BRASSINOSTEROID INSENSITIVE1 internalization can occur independent of ligand binding. PLANT PHYSIOLOGY, 192(1), 65–76. https://doi.org/10.1093/plphys/kiad005
Chicago author-date
Alves Neubus Claus, Lucas, Derui Liu, Ulrich Hohmann, Nemanja Vukasinovic, Roman Pleskot, Jing Liu, Alexei Schiffner, et al. 2023. “BRASSINOSTEROID INSENSITIVE1 Internalization Can Occur Independent of Ligand Binding.” PLANT PHYSIOLOGY 192 (1): 65–76. https://doi.org/10.1093/plphys/kiad005.
Chicago author-date (all authors)
Alves Neubus Claus, Lucas, Derui Liu, Ulrich Hohmann, Nemanja Vukasinovic, Roman Pleskot, Jing Liu, Alexei Schiffner, Yvon Jaillais, Guang Wu, Sebastian Wolf, Daniël Van Damme, Michael Hothorn, and Eugenia Russinova. 2023. “BRASSINOSTEROID INSENSITIVE1 Internalization Can Occur Independent of Ligand Binding.” PLANT PHYSIOLOGY 192 (1): 65–76. doi:10.1093/plphys/kiad005.
Vancouver
1.
Alves Neubus Claus L, Liu D, Hohmann U, Vukasinovic N, Pleskot R, Liu J, et al. BRASSINOSTEROID INSENSITIVE1 internalization can occur independent of ligand binding. PLANT PHYSIOLOGY. 2023;192(1):65–76.
IEEE
[1]
L. Alves Neubus Claus et al., “BRASSINOSTEROID INSENSITIVE1 internalization can occur independent of ligand binding,” PLANT PHYSIOLOGY, vol. 192, no. 1, pp. 65–76, 2023.
@article{01GTBQP81C96V9MJ2PQMDT0NAT,
  abstract     = {{The brassinosteroid (BR) hormone and its plasma membrane receptor BR INSENSITIVE1 (BRI1) is one of the best-studied receptor-ligand pairs for understanding the interplay between receptor endocytosis and signaling in plants. BR signaling is mainly determined by the plasma membrane pool of BRI1, whereas BRI1 endocytosis ensures signal attenuation. Since BRs are ubiquitously distributed in the plant, the tools available to study BRI1 function without interference from endogenous BRs are limited. Here, we designed a BR-binding-deficient Arabidopsis (Arabidopsis thaliana) mutant based on protein sequence-structure analysis and homology modeling of members of BRI1 family. This tool allowed us to re-examine the BRI1 endocytosis and signal attenuation model. We showed that despite impaired phosphorylation and ubiquitination, BR-binding-deficient BRI1 internalizes similarly to the wild-type form. Our data indicate that BRI1 internalization relies on different endocytic machinery. In addition, the BR-binding-deficient mutant provides opportunities to study non-canonical ligand-independent BRI1 functions.}},
  author       = {{Alves Neubus Claus, Lucas and Liu, Derui and Hohmann, Ulrich and Vukasinovic, Nemanja and Pleskot, Roman and Liu, Jing and Schiffner, Alexei and Jaillais, Yvon and Wu, Guang and Wolf, Sebastian and Van Damme, Daniël and Hothorn, Michael and Russinova, Eugenia}},
  issn         = {{0032-0889}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{BRI1,brassinosteroids,endocytosis,ligand-binding,non-canonical,EXTRACELLULAR DOMAIN,RECEPTOR,ENDOCYTOSIS,KINASE,PERCEPTION,TRAFFICKING,DEGRADATION,PROTEINS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{65--76}},
  title        = {{BRASSINOSTEROID INSENSITIVE1 internalization can occur independent of ligand binding}},
  url          = {{http://doi.org/10.1093/plphys/kiad005}},
  volume       = {{192}},
  year         = {{2023}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: