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A high dose of dietary berberine improves gut wall morphology, despite an expansion of enterobacteriaceae and a reduction in beneficial microbiota in broiler chickens

Tessa Dehau (UGent) , Marc Cherlet (UGent) , Siska Croubels (UGent) , Filip Van Immerseel (UGent) and Evy Goossens (UGent)
(2023) MSYSTEMS. 8(1).
Author
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Abstract
Dietary additives are widely used to reduce intestinal inflammation and enteritis, a growing problem in the broiler industry. Berberine, with anti-inflammatory, antioxidant, and antimicrobial activity, would be an interesting feed additive in this regard. Phytogenic products are embraced as alternatives to antimicrobials, and some are known to mitigate intestinal inflammation and ensure optimal gut health and performance in broiler chickens. Dietary inclusion of berberine, a benzylisoquinoline alkaloid found in plants, is believed to exert gut health-promoting effects through modulation of the gut microbiota; however, there are only a few studies investigating its effects in chickens. The aim of this study was to investigate the interplay between dietary supplementation of a high concentration of berberine, the gastrointestinal microbiota, and histomorphological parameters in the gut. Berberine was shown to increase villus length and decrease crypt depth and CD3(+) T-lymphocyte infiltration in the gut tissue of chickens at different ages. Berberine affected the diversity of the gut microbiota from the jejunum to the colon, both at a compositional and functional level, with larger effects observed in the large intestine. A high concentration of berberine enriched members of the Enterobacteriaceae family and depleted members of the Ruminococcaceae, Lachnospiraceae, and Peptostreptococcaceae families, as well as tended to reduce butyrate production in the cecum. In vivo results were confirmed by in vitro growth experiments, where increasing concentrations of berberine inhibited the growth of several butyrate-producing strains while not affecting that of Enterobacteriaceae strains. Positive correlations were found between berberine levels in plasma and villus length or villus-to-crypt ratio in the jejunum. Our study showed that berberine supplementation at a high concentration improves chicken gut morphology toward decreased inflammation, which is likely not mediated by the induced gut microbiota shifts.IMPORTANCE Dietary additives are widely used to reduce intestinal inflammation and enteritis, a growing problem in the broiler industry. Berberine, with anti-inflammatory, antioxidant, and antimicrobial activity, would be an interesting feed additive in this regard. This study investigates for the first time the impact of berberine supplementation on the chicken gastrointestinal microbiota, as a potential mechanism to improve gut health, together with histological effects in the small intestine. This study identified a dose-effect of berberine on the gut microbiota, indicating the importance of finding an optimal dose to be used as a dietary additive.
Keywords
Computer Science Applications, Genetics, Molecular Biology, Modeling and Simulation, Ecology, Evolution, Behavior and Systematics, Biochemistry, Physiology, Microbiology, 16S rRNA gene sequencing, gut microbiota, gut health, berberine

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MLA
Dehau, Tessa, et al. “A High Dose of Dietary Berberine Improves Gut Wall Morphology, despite an Expansion of Enterobacteriaceae and a Reduction in Beneficial Microbiota in Broiler Chickens.” MSYSTEMS, edited by Kathryn C. Milligan-McClellan, vol. 8, no. 1, American Society for Microbiology, 2023, doi:10.1128/msystems.01239-22.
APA
Dehau, T., Cherlet, M., Croubels, S., Van Immerseel, F., & Goossens, E. (2023). A high dose of dietary berberine improves gut wall morphology, despite an expansion of enterobacteriaceae and a reduction in beneficial microbiota in broiler chickens. MSYSTEMS, 8(1). https://doi.org/10.1128/msystems.01239-22
Chicago author-date
Dehau, Tessa, Marc Cherlet, Siska Croubels, Filip Van Immerseel, and Evy Goossens. 2023. “A High Dose of Dietary Berberine Improves Gut Wall Morphology, despite an Expansion of Enterobacteriaceae and a Reduction in Beneficial Microbiota in Broiler Chickens.” Edited by Kathryn C. Milligan-McClellan. MSYSTEMS 8 (1). https://doi.org/10.1128/msystems.01239-22.
Chicago author-date (all authors)
Dehau, Tessa, Marc Cherlet, Siska Croubels, Filip Van Immerseel, and Evy Goossens. 2023. “A High Dose of Dietary Berberine Improves Gut Wall Morphology, despite an Expansion of Enterobacteriaceae and a Reduction in Beneficial Microbiota in Broiler Chickens.” Ed by. Kathryn C. Milligan-McClellan. MSYSTEMS 8 (1). doi:10.1128/msystems.01239-22.
Vancouver
1.
Dehau T, Cherlet M, Croubels S, Van Immerseel F, Goossens E. A high dose of dietary berberine improves gut wall morphology, despite an expansion of enterobacteriaceae and a reduction in beneficial microbiota in broiler chickens. Milligan-McClellan KC, editor. MSYSTEMS. 2023;8(1).
IEEE
[1]
T. Dehau, M. Cherlet, S. Croubels, F. Van Immerseel, and E. Goossens, “A high dose of dietary berberine improves gut wall morphology, despite an expansion of enterobacteriaceae and a reduction in beneficial microbiota in broiler chickens,” MSYSTEMS, vol. 8, no. 1, 2023.
@article{01GT92GFRMZVKK99CBQM3DY4GK,
  abstract     = {{Dietary additives are widely used to reduce intestinal inflammation and enteritis, a growing problem in the broiler industry. Berberine, with anti-inflammatory, antioxidant, and antimicrobial activity, would be an interesting feed additive in this regard.

Phytogenic products are embraced as alternatives to antimicrobials, and some are known to mitigate intestinal inflammation and ensure optimal gut health and performance in broiler chickens. Dietary inclusion of berberine, a benzylisoquinoline alkaloid found in plants, is believed to exert gut health-promoting effects through modulation of the gut microbiota; however, there are only a few studies investigating its effects in chickens. The aim of this study was to investigate the interplay between dietary supplementation of a high concentration of berberine, the gastrointestinal microbiota, and histomorphological parameters in the gut. Berberine was shown to increase villus length and decrease crypt depth and CD3(+) T-lymphocyte infiltration in the gut tissue of chickens at different ages. Berberine affected the diversity of the gut microbiota from the jejunum to the colon, both at a compositional and functional level, with larger effects observed in the large intestine. A high concentration of berberine enriched members of the Enterobacteriaceae family and depleted members of the Ruminococcaceae, Lachnospiraceae, and Peptostreptococcaceae families, as well as tended to reduce butyrate production in the cecum. In vivo results were confirmed by in vitro growth experiments, where increasing concentrations of berberine inhibited the growth of several butyrate-producing strains while not affecting that of Enterobacteriaceae strains. Positive correlations were found between berberine levels in plasma and villus length or villus-to-crypt ratio in the jejunum. Our study showed that berberine supplementation at a high concentration improves chicken gut morphology toward decreased inflammation, which is likely not mediated by the induced gut microbiota shifts.IMPORTANCE Dietary additives are widely used to reduce intestinal inflammation and enteritis, a growing problem in the broiler industry. Berberine, with anti-inflammatory, antioxidant, and antimicrobial activity, would be an interesting feed additive in this regard. This study investigates for the first time the impact of berberine supplementation on the chicken gastrointestinal microbiota, as a potential mechanism to improve gut health, together with histological effects in the small intestine. This study identified a dose-effect of berberine on the gut microbiota, indicating the importance of finding an optimal dose to be used as a dietary additive.}},
  articleno    = {{e01239-22}},
  author       = {{Dehau, Tessa and Cherlet, Marc and Croubels, Siska and Van Immerseel, Filip and Goossens, Evy}},
  editor       = {{Milligan-McClellan, Kathryn C.}},
  issn         = {{2379-5077}},
  journal      = {{MSYSTEMS}},
  keywords     = {{Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology,16S rRNA gene sequencing,gut microbiota,gut health,berberine}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{25}},
  publisher    = {{American Society for Microbiology}},
  title        = {{A high dose of dietary berberine improves gut wall morphology, despite an expansion of enterobacteriaceae and a reduction in beneficial microbiota in broiler chickens}},
  url          = {{http://doi.org/10.1128/msystems.01239-22}},
  volume       = {{8}},
  year         = {{2023}},
}

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