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Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens

Ganna Saelens (UGent) , Sören Planckaert (UGent) , Victoria Martinez-Sernandez, Florencio M. Ubeira, Bart Devreese (UGent) and Sarah Gabriël (UGent)
(2022) SCIENTIFIC REPORTS. 12(1).
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Abstract
The family Anisakidae, mainly represented by Anisakis simplex s.l. and Pseudoterranova decipiens, encompasses zoonotic nematodes infecting many marine fish. Both are responsible for gastrointestinal disease in humans after ingestion of a live larva by consumption of undercooked fish, and, in the case of A. simplex, an allergic reaction may occur after consuming or even handling infected fish. Due to its phylogenetic relatedness with A. simplex, few studies investigated the allergenic potential of P. decipiens, yet none of them focused on its excretory/secretory (E/S) proteins that easily get missed when working solely on extracts from crushed nematodes. Moreover, these E/S allergens remain behind even when the larva has been removed during fish quality processing. Therefore, the aim was to investigate if Anisakis-like allergens could also be detected in both crushed and E/S P. decipiens protein extract using targeted mass spectrometry analysis and immunological methods. The results confirmed that at least five A. simplex allergens have homologous proteins in P. decipiens; a result that emphasizes the importance of also including E/S protein extracts in proteomic studies. Not only A. simplex, but also P. decipiens should therefore be considered a potential source of allergens that could lead to hypersensitivity reactions in humans.
Keywords
IDENTIFICATION, QUANTIFICATION, RECOMBINANT, INFECTION, DISCOVERY, PRODUCTS, PARASITE, FISH

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MLA
Saelens, Ganna, et al. “Targeted Proteomics and Specific Immunoassays Reveal the Presence of Shared Allergens between the Zoonotic Nematodes Anisakis Simplex and Pseudoterranova Decipiens.” SCIENTIFIC REPORTS, vol. 12, no. 1, 2022, doi:10.1038/s41598-022-08113-3.
APA
Saelens, G., Planckaert, S., Martinez-Sernandez, V., Ubeira, F. M., Devreese, B., & Gabriël, S. (2022). Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens. SCIENTIFIC REPORTS, 12(1). https://doi.org/10.1038/s41598-022-08113-3
Chicago author-date
Saelens, Ganna, Sören Planckaert, Victoria Martinez-Sernandez, Florencio M. Ubeira, Bart Devreese, and Sarah Gabriël. 2022. “Targeted Proteomics and Specific Immunoassays Reveal the Presence of Shared Allergens between the Zoonotic Nematodes Anisakis Simplex and Pseudoterranova Decipiens.” SCIENTIFIC REPORTS 12 (1). https://doi.org/10.1038/s41598-022-08113-3.
Chicago author-date (all authors)
Saelens, Ganna, Sören Planckaert, Victoria Martinez-Sernandez, Florencio M. Ubeira, Bart Devreese, and Sarah Gabriël. 2022. “Targeted Proteomics and Specific Immunoassays Reveal the Presence of Shared Allergens between the Zoonotic Nematodes Anisakis Simplex and Pseudoterranova Decipiens.” SCIENTIFIC REPORTS 12 (1). doi:10.1038/s41598-022-08113-3.
Vancouver
1.
Saelens G, Planckaert S, Martinez-Sernandez V, Ubeira FM, Devreese B, Gabriël S. Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens. SCIENTIFIC REPORTS. 2022;12(1).
IEEE
[1]
G. Saelens, S. Planckaert, V. Martinez-Sernandez, F. M. Ubeira, B. Devreese, and S. Gabriël, “Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens,” SCIENTIFIC REPORTS, vol. 12, no. 1, 2022.
@article{01GT1JGES7MPZ96EQRY1DS100S,
  abstract     = {{The family Anisakidae, mainly represented by Anisakis simplex s.l. and Pseudoterranova decipiens, encompasses zoonotic nematodes infecting many marine fish. Both are responsible for gastrointestinal disease in humans after ingestion of a live larva by consumption of undercooked fish, and, in the case of A. simplex, an allergic reaction may occur after consuming or even handling infected fish. Due to its phylogenetic relatedness with A. simplex, few studies investigated the allergenic potential of P. decipiens, yet none of them focused on its excretory/secretory (E/S) proteins that easily get missed when working solely on extracts from crushed nematodes. Moreover, these E/S allergens remain behind even when the larva has been removed during fish quality processing. Therefore, the aim was to investigate if Anisakis-like allergens could also be detected in both crushed and E/S P. decipiens protein extract using targeted mass spectrometry analysis and immunological methods. The results confirmed that at least five A. simplex allergens have homologous proteins in P. decipiens; a result that emphasizes the importance of also including E/S protein extracts in proteomic studies. Not only A. simplex, but also P. decipiens should therefore be considered a potential source of allergens that could lead to hypersensitivity reactions in humans.}},
  articleno    = {{4127}},
  author       = {{Saelens, Ganna and Planckaert, Sören and  Martinez-Sernandez, Victoria and  Ubeira, Florencio M. and Devreese, Bart and Gabriël, Sarah}},
  issn         = {{2045-2322}},
  journal      = {{SCIENTIFIC REPORTS}},
  keywords     = {{IDENTIFICATION,QUANTIFICATION,RECOMBINANT,INFECTION,DISCOVERY,PRODUCTS,PARASITE,FISH}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{11}},
  title        = {{Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens}},
  url          = {{http://doi.org/10.1038/s41598-022-08113-3}},
  volume       = {{12}},
  year         = {{2022}},
}
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