Building mutational bridges between carbohydrate-active enzymes
- Author
- Jorick Franceus (UGent) , Jolien Lormans (UGent) and Tom Desmet (UGent)
- Organization
- Project
- Abstract
- The commercial value of specialty carbohydrates and glycosylated compounds has sparked considerable interest in the synthetic potential of carbohydrate-active enzymes (CAZymes). Protein engineering methods have proven to be highly successful in expanding the range of glycosylation reactions that these enzymes can perform efficiently and costeffectively. The past few years have witnessed meaningful progress in this area, largely due to a sharper focus on the understanding of structure-function relationships and mechanistic intricacies. Here, we summarize recent studies that demonstrate how protein engineers have become much better at traversing the fitness landscape of CAZymes through mutational bridges that connect the different activity types.
- Keywords
- Biomedical Engineering, Bioengineering, Biotechnology, GLYCOSYLTRANSFERASE, PHOSPHORYLASES, HYDROLASES, CATALYSIS, FAMILY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GSQSCVBF8609WT3Q9626KGVR
- MLA
- Franceus, Jorick, et al. “Building Mutational Bridges between Carbohydrate-Active Enzymes.” CURRENT OPINION IN BIOTECHNOLOGY, vol. 78, 2022, doi:10.1016/j.copbio.2022.102804.
- APA
- Franceus, J., Lormans, J., & Desmet, T. (2022). Building mutational bridges between carbohydrate-active enzymes. CURRENT OPINION IN BIOTECHNOLOGY, 78. https://doi.org/10.1016/j.copbio.2022.102804
- Chicago author-date
- Franceus, Jorick, Jolien Lormans, and Tom Desmet. 2022. “Building Mutational Bridges between Carbohydrate-Active Enzymes.” CURRENT OPINION IN BIOTECHNOLOGY 78. https://doi.org/10.1016/j.copbio.2022.102804.
- Chicago author-date (all authors)
- Franceus, Jorick, Jolien Lormans, and Tom Desmet. 2022. “Building Mutational Bridges between Carbohydrate-Active Enzymes.” CURRENT OPINION IN BIOTECHNOLOGY 78. doi:10.1016/j.copbio.2022.102804.
- Vancouver
- 1.Franceus J, Lormans J, Desmet T. Building mutational bridges between carbohydrate-active enzymes. CURRENT OPINION IN BIOTECHNOLOGY. 2022;78.
- IEEE
- [1]J. Franceus, J. Lormans, and T. Desmet, “Building mutational bridges between carbohydrate-active enzymes,” CURRENT OPINION IN BIOTECHNOLOGY, vol. 78, 2022.
@article{01GSQSCVBF8609WT3Q9626KGVR,
abstract = {{The commercial value of specialty carbohydrates and glycosylated compounds has sparked considerable interest in the synthetic potential of carbohydrate-active enzymes (CAZymes). Protein engineering methods have proven to be highly successful in expanding the range of glycosylation reactions that these enzymes can perform efficiently and costeffectively. The past few years have witnessed meaningful progress in this area, largely due to a sharper focus on the understanding of structure-function relationships and mechanistic intricacies. Here, we summarize recent studies that demonstrate how protein engineers have become much better at traversing the fitness landscape of CAZymes through mutational bridges that connect the different activity types.}},
articleno = {{102804}},
author = {{Franceus, Jorick and Lormans, Jolien and Desmet, Tom}},
issn = {{0958-1669}},
journal = {{CURRENT OPINION IN BIOTECHNOLOGY}},
keywords = {{Biomedical Engineering,Bioengineering,Biotechnology,GLYCOSYLTRANSFERASE,PHOSPHORYLASES,HYDROLASES,CATALYSIS,FAMILY}},
language = {{eng}},
pages = {{7}},
title = {{Building mutational bridges between carbohydrate-active enzymes}},
url = {{http://doi.org/10.1016/j.copbio.2022.102804}},
volume = {{78}},
year = {{2022}},
}
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