Principles of antibodies with ultralong complementarity-determining regions and picobodies
- Author
- Marcel Fridolin Passon (UGent) , Stefaan De Smedt (UGent) and Hristo Svilenov (UGent)
- Organization
- Project
- Abstract
- In contrast to other species, cattle possess exceptional antibodies with ultra-long complementarity-determining regions (ulCDRs) that can consist of 40-70 amino acids. The bovine ulCDR is folded into a stalk and a disulfide-rich knob domain. The binding to the antigen is via the 3-6 kDa knob. There exists an immense sequence and structural diversity in the knob that enables binding to different antigens. Here we summarize the current knowledge of the ulCDR structure and provide an overview of the approaches to discover ulCDRs against novel antigens. Furthermore, we outline protein engineering approaches inspired by the natural ulCDRs. Finally, we discuss the enormous potential of using isolated bovine knobs, also named picobodies, as the smallest antigen-binding domains derived from natural antibodies.
- Keywords
- Biotechnology, Bioengineering, Applied Microbiology and Biotechnology, Antibodies, Complementarity-determining regions, Ultra-long CDRs, Exceptional CDRs, Bovine antibodies, Picobodies
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GSQSBQY6EM0W70V0SM3NT99K
- MLA
- Passon, Marcel Fridolin, et al. “Principles of Antibodies with Ultralong Complementarity-Determining Regions and Picobodies.” BIOTECHNOLOGY ADVANCES, vol. 64, Elsevier BV, 2023, doi:10.1016/j.biotechadv.2023.108120.
- APA
- Passon, M. F., De Smedt, S., & Svilenov, H. (2023). Principles of antibodies with ultralong complementarity-determining regions and picobodies. BIOTECHNOLOGY ADVANCES, 64. https://doi.org/10.1016/j.biotechadv.2023.108120
- Chicago author-date
- Passon, Marcel Fridolin, Stefaan De Smedt, and Hristo Svilenov. 2023. “Principles of Antibodies with Ultralong Complementarity-Determining Regions and Picobodies.” BIOTECHNOLOGY ADVANCES 64. https://doi.org/10.1016/j.biotechadv.2023.108120.
- Chicago author-date (all authors)
- Passon, Marcel Fridolin, Stefaan De Smedt, and Hristo Svilenov. 2023. “Principles of Antibodies with Ultralong Complementarity-Determining Regions and Picobodies.” BIOTECHNOLOGY ADVANCES 64. doi:10.1016/j.biotechadv.2023.108120.
- Vancouver
- 1.Passon MF, De Smedt S, Svilenov H. Principles of antibodies with ultralong complementarity-determining regions and picobodies. BIOTECHNOLOGY ADVANCES. 2023;64.
- IEEE
- [1]M. F. Passon, S. De Smedt, and H. Svilenov, “Principles of antibodies with ultralong complementarity-determining regions and picobodies,” BIOTECHNOLOGY ADVANCES, vol. 64, 2023.
@article{01GSQSBQY6EM0W70V0SM3NT99K,
abstract = {{In contrast to other species, cattle possess exceptional antibodies with ultra-long complementarity-determining regions (ulCDRs) that can consist of 40-70 amino acids. The bovine ulCDR is folded into a stalk and a disulfide-rich knob domain. The binding to the antigen is via the 3-6 kDa knob. There exists an immense sequence and structural diversity in the knob that enables binding to different antigens. Here we summarize the current knowledge of the ulCDR structure and provide an overview of the approaches to discover ulCDRs against novel antigens. Furthermore, we outline protein engineering approaches inspired by the natural ulCDRs. Finally, we discuss the enormous potential of using isolated bovine knobs, also named picobodies, as the smallest antigen-binding domains derived from natural antibodies.}},
articleno = {{108120}},
author = {{Passon, Marcel Fridolin and De Smedt, Stefaan and Svilenov, Hristo}},
issn = {{0734-9750}},
journal = {{BIOTECHNOLOGY ADVANCES}},
keywords = {{Biotechnology,Bioengineering,Applied Microbiology and Biotechnology,Antibodies,Complementarity-determining regions,Ultra-long CDRs,Exceptional CDRs,Bovine antibodies,Picobodies}},
language = {{eng}},
pages = {{11}},
publisher = {{Elsevier BV}},
title = {{Principles of antibodies with ultralong complementarity-determining regions and picobodies}},
url = {{http://doi.org/10.1016/j.biotechadv.2023.108120}},
volume = {{64}},
year = {{2023}},
}
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