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Bacterial quorum-sensing peptides as immune modulators present in systemic circulation

Anton De Spiegeleer (UGent) , Amélie Descamps (UGent) , Srinath Govindarajan (UGent) , Julie Coudenys (UGent) , Kevin Van der borght (UGent) , Hannah Hirmz (UGent) , Nele Van Den Noortgate (UGent) , Dirk Elewaut (UGent) , Bart De Spiegeleer and Evelien Wynendaele (UGent)
(2023) BIOMOLECULES. 13(2).
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Abstract
Quorum-sensing peptides (QSPs) are bacterial peptides traditionally considered only as inter-bacterial communication molecules. Recently, their involvement in microbiome-host interactions influencing host diseases such as cancer and sarcopenia were explored. However, it is still unknown to what extent these peptides have the potential to modulate the immune system. In this proof-of-concept study, we screened 89 QSPs for their potential to induce IL-6 and TNF alpha in murine splenocytes and J774 macrophages. Confirmatory experiments on the positive screening-hits were conducted using murine splenocytes and human PBMCs of different ages. Finally, to investigate the biological relevance of immunomodulatory QSPs, we analysed plasma in a human cohort for the presence of the immunomodulatory QSP Q010. To do this, we used a newly developed UHPLC-MS/MS method. Our findings indicated that specific QSPs activate immune cells in vitro, with Q007, Q010, Q017 and Q212 being the top four screening hits. Q007 and Q010 were affirmed in subsequent confirmatory experiments using murine splenocytes and human PBMCs. Finally, Q010 was detected in human plasma, demonstrating for the first time the presence of an immunomodulatory QSP in human circulation. In conclusion, our data are the first evidence indicating the potential of biologically relevant quorum-sensing peptides to modulate the immune system.
Keywords
Molecular Biology, Biochemistry, PBMC, splenocytes, J774, immune cells, microbiome, quorum-sensing peptides

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Citation

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MLA
De Spiegeleer, Anton, et al. “Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation.” BIOMOLECULES, vol. 13, no. 2, MDPI AG, 2023, doi:10.3390/biom13020296.
APA
De Spiegeleer, A., Descamps, A., Govindarajan, S., Coudenys, J., Van der borght, K., Hirmz, H., … Wynendaele, E. (2023). Bacterial quorum-sensing peptides as immune modulators present in systemic circulation. BIOMOLECULES, 13(2). https://doi.org/10.3390/biom13020296
Chicago author-date
De Spiegeleer, Anton, Amélie Descamps, Srinath Govindarajan, Julie Coudenys, Kevin Van der borght, Hannah Hirmz, Nele Van Den Noortgate, Dirk Elewaut, Bart De Spiegeleer, and Evelien Wynendaele. 2023. “Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation.” BIOMOLECULES 13 (2). https://doi.org/10.3390/biom13020296.
Chicago author-date (all authors)
De Spiegeleer, Anton, Amélie Descamps, Srinath Govindarajan, Julie Coudenys, Kevin Van der borght, Hannah Hirmz, Nele Van Den Noortgate, Dirk Elewaut, Bart De Spiegeleer, and Evelien Wynendaele. 2023. “Bacterial Quorum-Sensing Peptides as Immune Modulators Present in Systemic Circulation.” BIOMOLECULES 13 (2). doi:10.3390/biom13020296.
Vancouver
1.
De Spiegeleer A, Descamps A, Govindarajan S, Coudenys J, Van der borght K, Hirmz H, et al. Bacterial quorum-sensing peptides as immune modulators present in systemic circulation. BIOMOLECULES. 2023;13(2).
IEEE
[1]
A. De Spiegeleer et al., “Bacterial quorum-sensing peptides as immune modulators present in systemic circulation,” BIOMOLECULES, vol. 13, no. 2, 2023.
@article{01GSJYQV6Q4TVMWRY583XPTG0Z,
  abstract     = {{Quorum-sensing peptides (QSPs) are bacterial peptides traditionally considered only as inter-bacterial communication molecules. Recently, their involvement in microbiome-host interactions influencing host diseases such as cancer and sarcopenia were explored. However, it is still unknown to what extent these peptides have the potential to modulate the immune system. In this proof-of-concept study, we screened 89 QSPs for their potential to induce IL-6 and TNF alpha in murine splenocytes and J774 macrophages. Confirmatory experiments on the positive screening-hits were conducted using murine splenocytes and human PBMCs of different ages. Finally, to investigate the biological relevance of immunomodulatory QSPs, we analysed plasma in a human cohort for the presence of the immunomodulatory QSP Q010. To do this, we used a newly developed UHPLC-MS/MS method. Our findings indicated that specific QSPs activate immune cells in vitro, with Q007, Q010, Q017 and Q212 being the top four screening hits. Q007 and Q010 were affirmed in subsequent confirmatory experiments using murine splenocytes and human PBMCs. Finally, Q010 was detected in human plasma, demonstrating for the first time the presence of an immunomodulatory QSP in human circulation. In conclusion, our data are the first evidence indicating the potential of biologically relevant quorum-sensing peptides to modulate the immune system.}},
  articleno    = {{296}},
  author       = {{De Spiegeleer, Anton and Descamps, Amélie and Govindarajan, Srinath and Coudenys, Julie and Van der borght, Kevin and Hirmz, Hannah and Van Den Noortgate, Nele and Elewaut, Dirk and De Spiegeleer, Bart and Wynendaele, Evelien}},
  issn         = {{2218-273X}},
  journal      = {{BIOMOLECULES}},
  keywords     = {{Molecular Biology,Biochemistry,PBMC,splenocytes,J774,immune cells,microbiome,quorum-sensing peptides}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{14}},
  publisher    = {{MDPI AG}},
  title        = {{Bacterial quorum-sensing peptides as immune modulators present in systemic circulation}},
  url          = {{http://doi.org/10.3390/biom13020296}},
  volume       = {{13}},
  year         = {{2023}},
}
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