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Analytical performance of eight enzymatic assays for ethanol in serum evaluated by data from the Belgian external quality assessment scheme

Wim Coucke, Corinne Charlier, Kathleen Croes, Boris Mahieu, Hugo Neels, Christophe Stove (UGent) , Jan Tytgat, Andre Vanescote, Alain Verstraete (UGent) , Sarah Wille, et al.
(2022) CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 60(8). p.1211-1217
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Abstract
Objectives Fast and reliable ethanol assays analysis are used in a clinical context for patients suspected of ethanol intoxication. Mostly, automated systems using an enzymatic reaction based on ethanol dehydrogenase are used. The manuscript focusses on the evaluation of the performance of these assays. Methods Data included 30 serum samples used in the Belgian EQA scheme from 2019 to 2021 and concentrations ranged from 0.13 to 3.70 g/L. A regression line between target concentrations and reported values was calculated to evaluate outliers, bias, variability and measurement uncertainty. Results A total of 1,611 results were taken into account. Bias was the highest for Alinity c over the whole concentration range and the lowest for Vitros for low concentrations and Cobas 8000 using the c702 module for high concentrations. The Architect and Cobas c501/c502 systems showed the lowest variability over the whole concentration range. Highest variability was observed for Cobas 8000 using the 702 module, Thermo Scientific and Alinity c. Cobas 8000 using the c702 module showed the highest measurement uncertainty for lower concentrations. For higher concentrations, Alinity c, Thermo Scientific and Vitros were the methods with the highest measurement uncertainty. Conclusions The bias of the enzymatic techniques is nearly negligible for all methods except Alinity c. Variability differs strongly between measurement procedures. This study shows that the Alinity c has a worse measurement uncertainty than other systems for concentrations above 0.5 g/L. Overall, we found the differences in measurement uncertainty to be mainly influenced by the differences in variability.
Keywords
ethanol, external quality assessment, method evaluation

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MLA
Coucke, Wim, et al. “Analytical Performance of Eight Enzymatic Assays for Ethanol in Serum Evaluated by Data from the Belgian External Quality Assessment Scheme.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE, vol. 60, no. 8, 2022, pp. 1211–17, doi:10.1515/cclm-2022-0285.
APA
Coucke, W., Charlier, C., Croes, K., Mahieu, B., Neels, H., Stove, C., … Capron, A. (2022). Analytical performance of eight enzymatic assays for ethanol in serum evaluated by data from the Belgian external quality assessment scheme. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 60(8), 1211–1217. https://doi.org/10.1515/cclm-2022-0285
Chicago author-date
Coucke, Wim, Corinne Charlier, Kathleen Croes, Boris Mahieu, Hugo Neels, Christophe Stove, Jan Tytgat, et al. 2022. “Analytical Performance of Eight Enzymatic Assays for Ethanol in Serum Evaluated by Data from the Belgian External Quality Assessment Scheme.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE 60 (8): 1211–17. https://doi.org/10.1515/cclm-2022-0285.
Chicago author-date (all authors)
Coucke, Wim, Corinne Charlier, Kathleen Croes, Boris Mahieu, Hugo Neels, Christophe Stove, Jan Tytgat, Andre Vanescote, Alain Verstraete, Sarah Wille, and Arnaud Capron. 2022. “Analytical Performance of Eight Enzymatic Assays for Ethanol in Serum Evaluated by Data from the Belgian External Quality Assessment Scheme.” CLINICAL CHEMISTRY AND LABORATORY MEDICINE 60 (8): 1211–1217. doi:10.1515/cclm-2022-0285.
Vancouver
1.
Coucke W, Charlier C, Croes K, Mahieu B, Neels H, Stove C, et al. Analytical performance of eight enzymatic assays for ethanol in serum evaluated by data from the Belgian external quality assessment scheme. CLINICAL CHEMISTRY AND LABORATORY MEDICINE. 2022;60(8):1211–7.
IEEE
[1]
W. Coucke et al., “Analytical performance of eight enzymatic assays for ethanol in serum evaluated by data from the Belgian external quality assessment scheme,” CLINICAL CHEMISTRY AND LABORATORY MEDICINE, vol. 60, no. 8, pp. 1211–1217, 2022.
@article{01GS866C8FME1NZEXBG15WE1AF,
  abstract     = {{Objectives Fast and reliable ethanol assays analysis are used in a clinical context for patients suspected of ethanol intoxication. Mostly, automated systems using an enzymatic reaction based on ethanol dehydrogenase are used. The manuscript focusses on the evaluation of the performance of these assays. Methods Data included 30 serum samples used in the Belgian EQA scheme from 2019 to 2021 and concentrations ranged from 0.13 to 3.70 g/L. A regression line between target concentrations and reported values was calculated to evaluate outliers, bias, variability and measurement uncertainty. Results A total of 1,611 results were taken into account. Bias was the highest for Alinity c over the whole concentration range and the lowest for Vitros for low concentrations and Cobas 8000 using the c702 module for high concentrations. The Architect and Cobas c501/c502 systems showed the lowest variability over the whole concentration range. Highest variability was observed for Cobas 8000 using the 702 module, Thermo Scientific and Alinity c. Cobas 8000 using the c702 module showed the highest measurement uncertainty for lower concentrations. For higher concentrations, Alinity c, Thermo Scientific and Vitros were the methods with the highest measurement uncertainty. Conclusions The bias of the enzymatic techniques is nearly negligible for all methods except Alinity c. Variability differs strongly between measurement procedures. This study shows that the Alinity c has a worse measurement uncertainty than other systems for concentrations above 0.5 g/L. Overall, we found the differences in measurement uncertainty to be mainly influenced by the differences in variability.}},
  author       = {{Coucke, Wim and  Charlier, Corinne and  Croes, Kathleen and  Mahieu, Boris and  Neels, Hugo and Stove, Christophe and  Tytgat, Jan and  Vanescote, Andre and Verstraete, Alain and  Wille, Sarah and  Capron, Arnaud}},
  issn         = {{1434-6621}},
  journal      = {{CLINICAL CHEMISTRY AND LABORATORY MEDICINE}},
  keywords     = {{ethanol,external quality assessment,method evaluation}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1211--1217}},
  title        = {{Analytical performance of eight enzymatic assays for ethanol in serum evaluated by data from the Belgian external quality assessment scheme}},
  url          = {{http://doi.org/10.1515/cclm-2022-0285}},
  volume       = {{60}},
  year         = {{2022}},
}
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