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Studying whole-genome duplication using experimental evolution of Spirodela polyrhiza

Tian Wu (UGent) , Annelore Natran (UGent) , Lucas Prost (UGent) , Eylem Aydogdu Lohaus (UGent) , Yves Van de Peer (UGent) and Quinten Bafort (UGent)
(2023) Polyploidy : methods and protocols. In Methods in Molecular Biology 2545. p.373-390
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Abstract
In this chapter, we present the use of Spirodela polyrhiza in experiments designed to study the evolutionary impact of whole-genome duplication (WGD). We shortly introduce this duckweed species and explain why it is a suitable model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related duckweed experiment. These steps include strain selection, ploidy determination, different methods of making polyploid duckweeds, replication, culturing conditions, preservation, and the ways to quantify phenotypic and transcriptomic change.
Keywords
Duckweed; Evolve and resequence (E&R); Experimental evolution; Polyploidy; Spirodela polyrhiza; Whole-genome duplication.

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Citation

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MLA
Wu, Tian, et al. “Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela Polyrhiza.” Polyploidy : Methods and Protocols, vol. 2545, Humana, 2023, pp. 373–90, doi:10.1007/978-1-0716-2561-3_19.
APA
Wu, T., Natran, A., Prost, L., Aydogdu Lohaus, E., Van de Peer, Y., & Bafort, Q. (2023). Studying whole-genome duplication using experimental evolution of Spirodela polyrhiza. In Polyploidy : methods and protocols (Vol. 2545, pp. 373–390). https://doi.org/10.1007/978-1-0716-2561-3_19
Chicago author-date
Wu, Tian, Annelore Natran, Lucas Prost, Eylem Aydogdu Lohaus, Yves Van de Peer, and Quinten Bafort. 2023. “Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela Polyrhiza.” In Polyploidy : Methods and Protocols, 2545:373–90. New York, NY, USA: Humana. https://doi.org/10.1007/978-1-0716-2561-3_19.
Chicago author-date (all authors)
Wu, Tian, Annelore Natran, Lucas Prost, Eylem Aydogdu Lohaus, Yves Van de Peer, and Quinten Bafort. 2023. “Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela Polyrhiza.” In Polyploidy : Methods and Protocols, 2545:373–390. New York, NY, USA: Humana. doi:10.1007/978-1-0716-2561-3_19.
Vancouver
1.
Wu T, Natran A, Prost L, Aydogdu Lohaus E, Van de Peer Y, Bafort Q. Studying whole-genome duplication using experimental evolution of Spirodela polyrhiza. In: Polyploidy : methods and protocols. New York, NY, USA: Humana; 2023. p. 373–90.
IEEE
[1]
T. Wu, A. Natran, L. Prost, E. Aydogdu Lohaus, Y. Van de Peer, and Q. Bafort, “Studying whole-genome duplication using experimental evolution of Spirodela polyrhiza,” in Polyploidy : methods and protocols, vol. 2545, New York, NY, USA: Humana, 2023, pp. 373–390.
@incollection{01GS5DQ231WCNNQA2WJ0E290T8,
  abstract     = {{In this chapter, we present the use of Spirodela polyrhiza in experiments designed to study the evolutionary impact of whole-genome duplication (WGD). We shortly introduce this duckweed species and explain why it is a suitable model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related duckweed experiment. These steps include strain selection, ploidy determination, different methods of making polyploid duckweeds, replication, culturing conditions, preservation, and the ways to quantify phenotypic and transcriptomic change.}},
  author       = {{Wu, Tian and Natran, Annelore and Prost, Lucas and Aydogdu Lohaus, Eylem and Van de Peer, Yves and Bafort, Quinten}},
  booktitle    = {{Polyploidy : methods and protocols}},
  isbn         = {{9781071625606}},
  issn         = {{1064-3745}},
  keywords     = {{Duckweed; Evolve and resequence (E&R); Experimental evolution; Polyploidy; Spirodela polyrhiza; Whole-genome duplication.}},
  language     = {{eng}},
  pages        = {{373--390}},
  publisher    = {{Humana}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Studying whole-genome duplication using experimental evolution of Spirodela polyrhiza}},
  url          = {{http://doi.org/10.1007/978-1-0716-2561-3_19}},
  volume       = {{2545}},
  year         = {{2023}},
}
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