Studying whole-genome duplication using experimental evolution of Chlamydomonas
- Author
- Quinten Bafort (UGent) , Lucas Prost (UGent) , Eylem Aydogdu Lohaus (UGent) , Antoine Van de Vloet (UGent) , Griet Casteleyn (UGent) , Yves Van de Peer (UGent) and Olivier De Clerck (UGent)
- Organization
- Project
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- Studying the mechanistic underpinnings and applications of polyploidy through genomics, experimental evolution, modeling, and Artificial Intelligence
- ERC Professorship: DOUBLE-UP
- Testing big evolutionary hypotheses with small organisms
- experimental exploration of the uncertain road from whole genome duplication to evolutionary success
- When Genomes Team Up: investigation of the immediate effects of alloploidization on fitness and gene expression
- Abstract
- In this chapter, we present the use of Chlamydomonas reinhardtii in experiments designed to study the evolutionary impacts of whole genome duplication. We shortly introduce the algal species and depict why it is an excellent model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related Chlamydomonas experiment. These steps include strain selection, ploidy determination, different methods of making diplo- and polyploid Chlamydomonas cells, replication, culturing conditions, preservation, and the ways to quantify phenotypic and genotypic change.
- Keywords
- Chlamydomonas reinhardtii; Evolve and resequence (E&R); Experimental evolution; Polyploidy; Whole-genome duplication
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GS5AZ4VMJA233E8J2QPQWDBF
- MLA
- Bafort, Quinten, et al. “Studying Whole-Genome Duplication Using Experimental Evolution of Chlamydomonas.” Polyploidy : Methods and Protocols, edited by Yves Van de Peer, vol. 2545, Humana, 2023, pp. 351–72, doi:10.1007/978-1-0716-2561-3_18.
- APA
- Bafort, Q., Prost, L., Aydogdu Lohaus, E., Van de Vloet, A., Casteleyn, G., Van de Peer, Y., & De Clerck, O. (2023). Studying whole-genome duplication using experimental evolution of Chlamydomonas. In Y. Van de Peer (Ed.), Polyploidy : methods and protocols (Vol. 2545, pp. 351–372). https://doi.org/10.1007/978-1-0716-2561-3_18
- Chicago author-date
- Bafort, Quinten, Lucas Prost, Eylem Aydogdu Lohaus, Antoine Van de Vloet, Griet Casteleyn, Yves Van de Peer, and Olivier De Clerck. 2023. “Studying Whole-Genome Duplication Using Experimental Evolution of Chlamydomonas.” In Polyploidy : Methods and Protocols, edited by Yves Van de Peer, 2545:351–72. New York, NY, USA: Humana. https://doi.org/10.1007/978-1-0716-2561-3_18.
- Chicago author-date (all authors)
- Bafort, Quinten, Lucas Prost, Eylem Aydogdu Lohaus, Antoine Van de Vloet, Griet Casteleyn, Yves Van de Peer, and Olivier De Clerck. 2023. “Studying Whole-Genome Duplication Using Experimental Evolution of Chlamydomonas.” In Polyploidy : Methods and Protocols, ed by. Yves Van de Peer, 2545:351–372. New York, NY, USA: Humana. doi:10.1007/978-1-0716-2561-3_18.
- Vancouver
- 1.Bafort Q, Prost L, Aydogdu Lohaus E, Van de Vloet A, Casteleyn G, Van de Peer Y, et al. Studying whole-genome duplication using experimental evolution of Chlamydomonas. In: Van de Peer Y, editor. Polyploidy : methods and protocols. New York, NY, USA: Humana; 2023. p. 351–72.
- IEEE
- [1]Q. Bafort et al., “Studying whole-genome duplication using experimental evolution of Chlamydomonas,” in Polyploidy : methods and protocols, vol. 2545, Y. Van de Peer, Ed. New York, NY, USA: Humana, 2023, pp. 351–372.
@incollection{01GS5AZ4VMJA233E8J2QPQWDBF,
abstract = {{In this chapter, we present the use of Chlamydomonas reinhardtii in experiments designed to study the evolutionary impacts of whole genome duplication. We shortly introduce the algal species and depict why it is an excellent model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related Chlamydomonas experiment. These steps include strain selection, ploidy determination, different methods of making diplo- and polyploid Chlamydomonas cells, replication, culturing conditions, preservation, and the ways to quantify phenotypic and genotypic change.}},
author = {{Bafort, Quinten and Prost, Lucas and Aydogdu Lohaus, Eylem and Van de Vloet, Antoine and Casteleyn, Griet and Van de Peer, Yves and De Clerck, Olivier}},
booktitle = {{Polyploidy : methods and protocols}},
editor = {{Van de Peer, Yves}},
isbn = {{9781071625606}},
issn = {{1064-3745}},
keywords = {{Chlamydomonas reinhardtii; Evolve and resequence (E&R); Experimental evolution; Polyploidy; Whole-genome duplication}},
language = {{eng}},
pages = {{351--372}},
publisher = {{Humana}},
series = {{Methods in Molecular Biology}},
title = {{Studying whole-genome duplication using experimental evolution of Chlamydomonas}},
url = {{http://doi.org/10.1007/978-1-0716-2561-3_18}},
volume = {{2545}},
year = {{2023}},
}
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