Evaluating lncRNA expression patterns during HIV-1 treatment interruption
- Author
- Tinus Schynkel (UGent) , Willem van Snippenberg, Clarissa Van Hecke, Linos Vandekerckhove (UGent) and Wim Trypsteen (UGent)
- Organization
- Project
- Abstract
- Lately, the interest in long non-coding RNAs (lncRNAs) as potential drug targets and predictive markers in the context of HIV-1 has peaked, but their in vivo expression and regulation remains largely unexplored. Therefore, the present study examined lncRNA expression patterns during a clinical antiretroviral treatment interruption (ATI) trial. Peripheral blood mononuclear cells were isolated from ten patients at four timepoints: prior to ATI, 7-15 days after stop, at viral rebound and 3 months post antiretroviral therapy re-initiation. RNA was extracted and RT-qPCR on five known HIV-1-related lncRNAs (HEAL, MALAT1, NEAT1, GAS5 and NRON) was performed and correlated with HIV-1 and host marker expression. All lncRNAs correlated stronger with interferon stimulated genes (ISGs) than with HIV-1 reservoir and replication markers. However, one lncRNA, HEAL, showed significant upregulation at viral rebound during ATI compared to baseline and re-initiation of therapy (p = 0.0010 and p = 0.0094, respectively), following a similar viral-load-driven expression pattern to ISGs. In vitro knockdown of HEAL caused a significant reduction in HIV-1 infection levels, validating HEAL's importance for HIV-1 replication. We conclude that the HIV-1-promoting lncRNA HEAL is upregulated at viral rebound during ATI, most likely induced by viral cues.
- Keywords
- NONCODING RNAS, BLOOD, lncRNA, HIV-1, treatment interruption, biomarker, HEAL, MALAT1, NEAT1, GAS5, NRON
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GS551VA5TGVQM69BWW0QF70R
- MLA
- Schynkel, Tinus, et al. “Evaluating LncRNA Expression Patterns during HIV-1 Treatment Interruption.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 2, 2023, doi:10.3390/ijms24021031.
- APA
- Schynkel, T., van Snippenberg, W., Van Hecke, C., Vandekerckhove, L., & Trypsteen, W. (2023). Evaluating lncRNA expression patterns during HIV-1 treatment interruption. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(2). https://doi.org/10.3390/ijms24021031
- Chicago author-date
- Schynkel, Tinus, Willem van Snippenberg, Clarissa Van Hecke, Linos Vandekerckhove, and Wim Trypsteen. 2023. “Evaluating LncRNA Expression Patterns during HIV-1 Treatment Interruption.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (2). https://doi.org/10.3390/ijms24021031.
- Chicago author-date (all authors)
- Schynkel, Tinus, Willem van Snippenberg, Clarissa Van Hecke, Linos Vandekerckhove, and Wim Trypsteen. 2023. “Evaluating LncRNA Expression Patterns during HIV-1 Treatment Interruption.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (2). doi:10.3390/ijms24021031.
- Vancouver
- 1.Schynkel T, van Snippenberg W, Van Hecke C, Vandekerckhove L, Trypsteen W. Evaluating lncRNA expression patterns during HIV-1 treatment interruption. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2023;24(2).
- IEEE
- [1]T. Schynkel, W. van Snippenberg, C. Van Hecke, L. Vandekerckhove, and W. Trypsteen, “Evaluating lncRNA expression patterns during HIV-1 treatment interruption,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 2, 2023.
@article{01GS551VA5TGVQM69BWW0QF70R,
abstract = {{Lately, the interest in long non-coding RNAs (lncRNAs) as potential drug targets and predictive markers in the context of HIV-1 has peaked, but their in vivo expression and regulation remains largely unexplored. Therefore, the present study examined lncRNA expression patterns during a clinical antiretroviral treatment interruption (ATI) trial. Peripheral blood mononuclear cells were isolated from ten patients at four timepoints: prior to ATI, 7-15 days after stop, at viral rebound and 3 months post antiretroviral therapy re-initiation. RNA was extracted and RT-qPCR on five known HIV-1-related lncRNAs (HEAL, MALAT1, NEAT1, GAS5 and NRON) was performed and correlated with HIV-1 and host marker expression. All lncRNAs correlated stronger with interferon stimulated genes (ISGs) than with HIV-1 reservoir and replication markers. However, one lncRNA, HEAL, showed significant upregulation at viral rebound during ATI compared to baseline and re-initiation of therapy (p = 0.0010 and p = 0.0094, respectively), following a similar viral-load-driven expression pattern to ISGs. In vitro knockdown of HEAL caused a significant reduction in HIV-1 infection levels, validating HEAL's importance for HIV-1 replication. We conclude that the HIV-1-promoting lncRNA HEAL is upregulated at viral rebound during ATI, most likely induced by viral cues.}},
articleno = {{1031}},
author = {{Schynkel, Tinus and van Snippenberg, Willem and Van Hecke, Clarissa and Vandekerckhove, Linos and Trypsteen, Wim}},
issn = {{1422-0067}},
journal = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
keywords = {{NONCODING RNAS,BLOOD,lncRNA,HIV-1,treatment interruption,biomarker,HEAL,MALAT1,NEAT1,GAS5,NRON}},
language = {{eng}},
number = {{2}},
pages = {{13}},
title = {{Evaluating lncRNA expression patterns during HIV-1 treatment interruption}},
url = {{http://doi.org/10.3390/ijms24021031}},
volume = {{24}},
year = {{2023}},
}
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