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ABCB‐mediated shootward auxin transport feeds into the root clock

Jian Chen (UGent) , Yangjie Hu, Pengchao Hao, Tashi Tsering, Jian Xia, Yuqin Zhang, Ohad Roth, Maria Njo (UGent) , Lieven Sterck (UGent) , Yun Hu, et al.
(2023) EMBO REPORTS. 24(4).
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Abstract
Although strongly influenced by environmental conditions, lateral root (LR) positioning along the primary root appears to follow obediently an internal spacing mechanism dictated by auxin oscillations that prepattern the primary root, referred to as the root clock. Surprisingly, none of the hitherto characterized PIN- and ABCB-type auxin transporters seem to be involved in this LR prepatterning mechanism. Here, we characterize ABCB15, 16, 17, 18, and 22 (ABCB15-22) as novel auxin-transporting ABCBs. Knock-down and genome editing of this genetically linked group of ABCBs caused strongly reduced LR densities. These phenotypes were correlated with reduced amplitude, but not reduced frequency of the root clock oscillation. High-resolution auxin transport assays and tissue-specific silencing revealed contributions of ABCB15-22 to shootward auxin transport in the lateral root cap (LRC) and epidermis, thereby explaining the reduced auxin oscillation. Jointly, these data support a model in which LRC-derived auxin contributes to the root clock amplitude.
Keywords
ABCB, auxin transport, lateral root, root meristem, LATERAL ROOT, GENE-EXPRESSION, ARTIFICIAL MICRORNA, P-GLYCOPROTEIN, WEB TOOL, ARABIDOPSIS, RESISTANCE, PIN, TRANSFORMATION, IMMUNOPHILIN

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MLA
Chen, Jian, et al. “ABCB‐mediated Shootward Auxin Transport Feeds into the Root Clock.” EMBO REPORTS, vol. 24, no. 4, 2023, doi:10.15252/embr.202256271.
APA
Chen, J., Hu, Y., Hao, P., Tsering, T., Xia, J., Zhang, Y., … Vanneste, S. (2023). ABCB‐mediated shootward auxin transport feeds into the root clock. EMBO REPORTS, 24(4). https://doi.org/10.15252/embr.202256271
Chicago author-date
Chen, Jian, Yangjie Hu, Pengchao Hao, Tashi Tsering, Jian Xia, Yuqin Zhang, Ohad Roth, et al. 2023. “ABCB‐mediated Shootward Auxin Transport Feeds into the Root Clock.” EMBO REPORTS 24 (4). https://doi.org/10.15252/embr.202256271.
Chicago author-date (all authors)
Chen, Jian, Yangjie Hu, Pengchao Hao, Tashi Tsering, Jian Xia, Yuqin Zhang, Ohad Roth, Maria Njo, Lieven Sterck, Yun Hu, Yunde Zhao, Danny Geelen, Markus Geisler, Eilon Shani, Tom Beeckman, and Steffen Vanneste. 2023. “ABCB‐mediated Shootward Auxin Transport Feeds into the Root Clock.” EMBO REPORTS 24 (4). doi:10.15252/embr.202256271.
Vancouver
1.
Chen J, Hu Y, Hao P, Tsering T, Xia J, Zhang Y, et al. ABCB‐mediated shootward auxin transport feeds into the root clock. EMBO REPORTS. 2023;24(4).
IEEE
[1]
J. Chen et al., “ABCB‐mediated shootward auxin transport feeds into the root clock,” EMBO REPORTS, vol. 24, no. 4, 2023.
@article{01GRBC1WRFTSJETDXDM4195NE8,
  abstract     = {{Although strongly influenced by environmental conditions, lateral root (LR) positioning along the primary root appears to follow obediently an internal spacing mechanism dictated by auxin oscillations that prepattern the primary root, referred to as the root clock. Surprisingly, none of the hitherto characterized PIN- and ABCB-type auxin transporters seem to be involved in this LR prepatterning mechanism. Here, we characterize ABCB15, 16, 17, 18, and 22 (ABCB15-22) as novel auxin-transporting ABCBs. Knock-down and genome editing of this genetically linked group of ABCBs caused strongly reduced LR densities. These phenotypes were correlated with reduced amplitude, but not reduced frequency of the root clock oscillation. High-resolution auxin transport assays and tissue-specific silencing revealed contributions of ABCB15-22 to shootward auxin transport in the lateral root cap (LRC) and epidermis, thereby explaining the reduced auxin oscillation. Jointly, these data support a model in which LRC-derived auxin contributes to the root clock amplitude.}},
  articleno    = {{e56271}},
  author       = {{Chen, Jian and Hu, Yangjie and Hao, Pengchao and Tsering, Tashi and Xia, Jian and Zhang, Yuqin and Roth, Ohad and Njo, Maria and Sterck, Lieven and Hu, Yun and Zhao, Yunde and Geelen, Danny and Geisler, Markus and Shani, Eilon and Beeckman, Tom and Vanneste, Steffen}},
  issn         = {{1469-221X}},
  journal      = {{EMBO REPORTS}},
  keywords     = {{ABCB,auxin transport,lateral root,root meristem,LATERAL ROOT,GENE-EXPRESSION,ARTIFICIAL MICRORNA,P-GLYCOPROTEIN,WEB TOOL,ARABIDOPSIS,RESISTANCE,PIN,TRANSFORMATION,IMMUNOPHILIN}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{16}},
  title        = {{ABCB‐mediated shootward auxin transport feeds into the root clock}},
  url          = {{http://doi.org/10.15252/embr.202256271}},
  volume       = {{24}},
  year         = {{2023}},
}
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