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Glycosidic linkage of rare and new-to-nature disaccharides reshapes gut microbiota in vitro

Stanley Omondi Onyango, Koen Beerens (UGent) , Qiqiong Li (UGent) , John Van Camp (UGent) , Tom Desmet (UGent) and Tom Van de Wiele (UGent)
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Abstract
The impact of glycosidic linkage of seven rare and new-to-nature disaccharides on gut bacteria was assessed in vitro. The community shift of the inocula from four donors in response to 1 % (w/v) disaccharide supplemen-tation was captured by sequencing the 16S rRNA gene. A significant loss of bacterial alpha diversity, short lag time, low pH, and high total short-chain fatty acid displayed a faster fermentation of trehalose(Glc-alpha 1,1 alpha-Glc) and fibrulose(fructan, DP2-10). Bacteroides reduced in relative abundance under disaccharide supplementation suggesting a loss in complex carbohydrates metabolizing capacity. Fibrulose and L-arabinose glucoside(Glc-alpha 1,3-L-Ara) significantly stimulated bifidobacteria but was suppressed with trehalose, ribose glucoside(Glc-alpha 1,2-Rib), and 4 '-epitrehalose(Glc-alpha 1,1 alpha-Gal) supplementation. Albeit insignificant, bifidobacteria increased with 4 '-epi-kojibiose(Glc-alpha 1,2-Gal), nigerose(Glc-alpha 1,3-Glc), and kojibiose(Glc-alpha 1,2-Glc). Prior conditioning of inoculum in kojibiose medium profoundly induced bifidobacteria by 44 % and 55 % upon reinoculation into kojibiose and fibrulose-supplemented media respectively. This study has demonstrated the importance of the disaccharide structure-function relationship in driving the gut bacterial community.
Keywords
General Medicine, Food Science, Analytical Chemistry, Prebiotics, Gut microbiota, Fibrulose, Trehalose, Kojibiose, Glycosidic linkage, Disaccharides

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Citation

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MLA
Onyango, Stanley Omondi, et al. “Glycosidic Linkage of Rare and New-to-Nature Disaccharides Reshapes Gut Microbiota in Vitro.” FOOD CHEMISTRY, vol. 411, 2023, doi:10.1016/j.foodchem.2023.135440.
APA
Onyango, S. O., Beerens, K., Li, Q., Van Camp, J., Desmet, T., & Van de Wiele, T. (2023). Glycosidic linkage of rare and new-to-nature disaccharides reshapes gut microbiota in vitro. FOOD CHEMISTRY, 411. https://doi.org/10.1016/j.foodchem.2023.135440
Chicago author-date
Onyango, Stanley Omondi, Koen Beerens, Qiqiong Li, John Van Camp, Tom Desmet, and Tom Van de Wiele. 2023. “Glycosidic Linkage of Rare and New-to-Nature Disaccharides Reshapes Gut Microbiota in Vitro.” FOOD CHEMISTRY 411. https://doi.org/10.1016/j.foodchem.2023.135440.
Chicago author-date (all authors)
Onyango, Stanley Omondi, Koen Beerens, Qiqiong Li, John Van Camp, Tom Desmet, and Tom Van de Wiele. 2023. “Glycosidic Linkage of Rare and New-to-Nature Disaccharides Reshapes Gut Microbiota in Vitro.” FOOD CHEMISTRY 411. doi:10.1016/j.foodchem.2023.135440.
Vancouver
1.
Onyango SO, Beerens K, Li Q, Van Camp J, Desmet T, Van de Wiele T. Glycosidic linkage of rare and new-to-nature disaccharides reshapes gut microbiota in vitro. FOOD CHEMISTRY. 2023;411.
IEEE
[1]
S. O. Onyango, K. Beerens, Q. Li, J. Van Camp, T. Desmet, and T. Van de Wiele, “Glycosidic linkage of rare and new-to-nature disaccharides reshapes gut microbiota in vitro,” FOOD CHEMISTRY, vol. 411, 2023.
@article{01GR40Y8MKTEB2S01X6Y9M6CXH,
  abstract     = {{The impact of glycosidic linkage of seven rare and new-to-nature disaccharides on gut bacteria was assessed in vitro. The community shift of the inocula from four donors in response to 1 % (w/v) disaccharide supplemen-tation was captured by sequencing the 16S rRNA gene. A significant loss of bacterial alpha diversity, short lag time, low pH, and high total short-chain fatty acid displayed a faster fermentation of trehalose(Glc-alpha 1,1 alpha-Glc) and fibrulose(fructan, DP2-10). Bacteroides reduced in relative abundance under disaccharide supplementation suggesting a loss in complex carbohydrates metabolizing capacity. Fibrulose and L-arabinose glucoside(Glc-alpha 1,3-L-Ara) significantly stimulated bifidobacteria but was suppressed with trehalose, ribose glucoside(Glc-alpha 1,2-Rib), and 4 '-epitrehalose(Glc-alpha 1,1 alpha-Gal) supplementation. Albeit insignificant, bifidobacteria increased with 4 '-epi-kojibiose(Glc-alpha 1,2-Gal), nigerose(Glc-alpha 1,3-Glc), and kojibiose(Glc-alpha 1,2-Glc). Prior conditioning of inoculum in kojibiose medium profoundly induced bifidobacteria by 44 % and 55 % upon reinoculation into kojibiose and fibrulose-supplemented media respectively. This study has demonstrated the importance of the disaccharide structure-function relationship in driving the gut bacterial community.}},
  articleno    = {{135440}},
  author       = {{Onyango, Stanley Omondi and Beerens, Koen and Li, Qiqiong and Van Camp, John and Desmet, Tom and Van de Wiele, Tom}},
  issn         = {{0308-8146}},
  journal      = {{FOOD CHEMISTRY}},
  keywords     = {{General Medicine,Food Science,Analytical Chemistry,Prebiotics,Gut microbiota,Fibrulose,Trehalose,Kojibiose,Glycosidic linkage,Disaccharides}},
  language     = {{eng}},
  pages        = {{11}},
  title        = {{Glycosidic linkage of rare and new-to-nature disaccharides reshapes gut microbiota in vitro}},
  url          = {{http://doi.org/10.1016/j.foodchem.2023.135440}},
  volume       = {{411}},
  year         = {{2023}},
}

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