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Exploration of archaeal nucleotide sugar epimerases unveils a new and highly promiscuous GDP-Gal4E subgroup

Carlos Alvarez Quispe (UGent) , Matthieu Da Costa (UGent) , Koen Beerens (UGent) and Tom Desmet (UGent)
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Abstract
UDP-galactose 4-epimerase (Gal4E, EC 5.1.3.2) catalyses the interconversion of UDP-galactose and UDPglucose by inverting the configuration of the 4'-hydroxyl group of the sugar moiety. This enzyme is one of the mechanistically best-characterized members of the Short-chain Dehydrogenase/Reductase (SDR) superfamily. Although Gal4E was widely studied throughout all domains of life, ranging from eukaryotes to archaea, its biochemical characterization was often limited to UDP-hexoses, neglecting the possibility that Gal4E might be promiscuous towards other NDP-sugars and derivatives thereof. In this study, we identified a novel Gal4E subgroup displaying an unprecedented specificity on guanosine diphosphate (GDP) sugars. As a proof of concept, a detailed biochemical investigation was performed on Gal4E from Pyrococcus horikoshii (phGal4E_1), which revealed that it has, in fact, a clear preference for GDP-sugars. In addition, we confirmed that it accepts a variety of other sugar moieties, including L-sugars like L-galactose and L-fucose.
Keywords
Biotechnology, CEP1, UDP-galactose 4-epimerase, L-sugars, Promiscuity, Archaeal enzymes

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MLA
Alvarez Quispe, Carlos, et al. “Exploration of Archaeal Nucleotide Sugar Epimerases Unveils a New and Highly Promiscuous GDP-Gal4E Subgroup.” CURRENT RESEARCH IN BIOTECHNOLOGY, vol. 4, 2022, pp. 350–58, doi:10.1016/j.crbiot.2022.08.003.
APA
Alvarez Quispe, C., Da Costa, M., Beerens, K., & Desmet, T. (2022). Exploration of archaeal nucleotide sugar epimerases unveils a new and highly promiscuous GDP-Gal4E subgroup. CURRENT RESEARCH IN BIOTECHNOLOGY, 4, 350–358. https://doi.org/10.1016/j.crbiot.2022.08.003
Chicago author-date
Alvarez Quispe, Carlos, Matthieu Da Costa, Koen Beerens, and Tom Desmet. 2022. “Exploration of Archaeal Nucleotide Sugar Epimerases Unveils a New and Highly Promiscuous GDP-Gal4E Subgroup.” CURRENT RESEARCH IN BIOTECHNOLOGY 4: 350–58. https://doi.org/10.1016/j.crbiot.2022.08.003.
Chicago author-date (all authors)
Alvarez Quispe, Carlos, Matthieu Da Costa, Koen Beerens, and Tom Desmet. 2022. “Exploration of Archaeal Nucleotide Sugar Epimerases Unveils a New and Highly Promiscuous GDP-Gal4E Subgroup.” CURRENT RESEARCH IN BIOTECHNOLOGY 4: 350–358. doi:10.1016/j.crbiot.2022.08.003.
Vancouver
1.
Alvarez Quispe C, Da Costa M, Beerens K, Desmet T. Exploration of archaeal nucleotide sugar epimerases unveils a new and highly promiscuous GDP-Gal4E subgroup. CURRENT RESEARCH IN BIOTECHNOLOGY. 2022;4:350–8.
IEEE
[1]
C. Alvarez Quispe, M. Da Costa, K. Beerens, and T. Desmet, “Exploration of archaeal nucleotide sugar epimerases unveils a new and highly promiscuous GDP-Gal4E subgroup,” CURRENT RESEARCH IN BIOTECHNOLOGY, vol. 4, pp. 350–358, 2022.
@article{01GQSZWS2CAPR7ZEP471RK4S84,
  abstract     = {{UDP-galactose 4-epimerase (Gal4E, EC 5.1.3.2) catalyses the interconversion of UDP-galactose and UDPglucose by inverting the configuration of the 4'-hydroxyl group of the sugar moiety. This enzyme is one of the mechanistically best-characterized members of the Short-chain Dehydrogenase/Reductase (SDR) superfamily. Although Gal4E was widely studied throughout all domains of life, ranging from eukaryotes to archaea, its biochemical characterization was often limited to UDP-hexoses, neglecting the possibility that Gal4E might be promiscuous towards other NDP-sugars and derivatives thereof. In this study, we identified a novel Gal4E subgroup displaying an unprecedented specificity on guanosine diphosphate (GDP) sugars. As a proof of concept, a detailed biochemical investigation was performed on Gal4E from Pyrococcus horikoshii (phGal4E_1), which revealed that it has, in fact, a clear preference for GDP-sugars. In addition, we confirmed that it accepts a variety of other sugar moieties, including L-sugars like L-galactose and L-fucose.}},
  author       = {{Alvarez Quispe, Carlos and Da Costa, Matthieu and Beerens, Koen and Desmet, Tom}},
  issn         = {{2590-2628}},
  journal      = {{CURRENT RESEARCH IN BIOTECHNOLOGY}},
  keywords     = {{Biotechnology,CEP1,UDP-galactose 4-epimerase,L-sugars,Promiscuity,Archaeal enzymes}},
  language     = {{eng}},
  pages        = {{350--358}},
  title        = {{Exploration of archaeal nucleotide sugar epimerases unveils a new and highly promiscuous GDP-Gal4E subgroup}},
  url          = {{http://doi.org/10.1016/j.crbiot.2022.08.003}},
  volume       = {{4}},
  year         = {{2022}},
}

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