Advanced search
1 file | 3.50 MB Add to list

Spontaneous shrinkage drives macromolecule encapsulation into layer-by-layer assembled biopolymer microgels

Author
Organization
Project
Abstract
Hypothesis: Recently, the anomalous shrinkage of surface-supported hyaluronate/poly-L-lysine (HA/PLL) microgels (mu-gels), which exceeds that reported for any other multilayer-based systems, has been reported [1]. The current study investigates the capability of these unique mu-gels for the encapsulation and retention of macromolecules, and proposes the shrinkage-driven assembly of biopolymer-based mu- gels as a novel tool for one-step surface biofunctionalization.Experiments: A set of dextrans (DEX) and their charged derivatives -carboxymethyl (CM)-DEX and diethylaminoethyl (DEAE)-DEX -has been utilized to evaluate the effects of macromolecular mass and net charge on mu-gel shrinkage and macromolecule entrapment. mu-gels formation on the surface of silicone catheters exemplifies their potential to tailor biointerfaces.Findings: Shrinkage-driven mu-gel formation strongly depends on the net charge and mass content of encapsulated macromolecules. Inclusion of neutral DEX decreases the degree of shrinkage several times, whilst charged DEXs adopt to the backbone of oppositely charged polyelectrolytes, resulting in shrinkage comparable to that of non-loaded mu-gels. Retention of CM-DEX in mu-gels is significantly higher compared to DEAE-DEX. These insights into the mechanisms of macromolecular entrapment into biopolymer-based mu-gels promotes fundamental understanding of molecular dynamics within the multilayer assemblies. Organization of biodegradable mu-gels at biomaterial surfaces opens avenues for their further exploitation in a diverse array of bioapplications.(c) 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords
Surface functionalization, Vaterite, Hard templating, Encapsulation, Multilayer, MULTILAYER CAPSULES, DRUG-DELIVERY, VATERITE CACO3, POLYELECTROLYTE, SIZE, MICROCAPSULES, FILMS

Downloads

  • J Colloid & Interface Sci-2023 1-s2.0-S0021979722022639-main.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 3.50 MB

Citation

Please use this url to cite or link to this publication:

MLA
Campbell, Jack, et al. “Spontaneous Shrinkage Drives Macromolecule Encapsulation into Layer-by-Layer Assembled Biopolymer Microgels.” JOURNAL OF COLLOID AND INTERFACE SCIENCE, vol. 635, 2023, pp. 12–22, doi:10.1016/j.jcis.2022.12.115.
APA
Campbell, J., Taghavi, A., Preis, A., Martin, S., Skirtach, A., Franke, J., … Vikulina, A. (2023). Spontaneous shrinkage drives macromolecule encapsulation into layer-by-layer assembled biopolymer microgels. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 635, 12–22. https://doi.org/10.1016/j.jcis.2022.12.115
Chicago author-date
Campbell, Jack, Aaron Taghavi, Alexander Preis, Sina Martin, Andre Skirtach, Jorg Franke, Dmitry Volodkin, and Anna Vikulina. 2023. “Spontaneous Shrinkage Drives Macromolecule Encapsulation into Layer-by-Layer Assembled Biopolymer Microgels.” JOURNAL OF COLLOID AND INTERFACE SCIENCE 635: 12–22. https://doi.org/10.1016/j.jcis.2022.12.115.
Chicago author-date (all authors)
Campbell, Jack, Aaron Taghavi, Alexander Preis, Sina Martin, Andre Skirtach, Jorg Franke, Dmitry Volodkin, and Anna Vikulina. 2023. “Spontaneous Shrinkage Drives Macromolecule Encapsulation into Layer-by-Layer Assembled Biopolymer Microgels.” JOURNAL OF COLLOID AND INTERFACE SCIENCE 635: 12–22. doi:10.1016/j.jcis.2022.12.115.
Vancouver
1.
Campbell J, Taghavi A, Preis A, Martin S, Skirtach A, Franke J, et al. Spontaneous shrinkage drives macromolecule encapsulation into layer-by-layer assembled biopolymer microgels. JOURNAL OF COLLOID AND INTERFACE SCIENCE. 2023;635:12–22.
IEEE
[1]
J. Campbell et al., “Spontaneous shrinkage drives macromolecule encapsulation into layer-by-layer assembled biopolymer microgels,” JOURNAL OF COLLOID AND INTERFACE SCIENCE, vol. 635, pp. 12–22, 2023.
@article{01GQHGBF6HD5KACDG0XZ6EYSN7,
  abstract     = {{Hypothesis: Recently, the anomalous shrinkage of surface-supported hyaluronate/poly-L-lysine (HA/PLL) microgels (mu-gels), which exceeds that reported for any other multilayer-based systems, has been reported [1]. The current study investigates the capability of these unique mu-gels for the encapsulation and retention of macromolecules, and proposes the shrinkage-driven assembly of biopolymer-based mu- gels as a novel tool for one-step surface biofunctionalization.Experiments: A set of dextrans (DEX) and their charged derivatives -carboxymethyl (CM)-DEX and diethylaminoethyl (DEAE)-DEX -has been utilized to evaluate the effects of macromolecular mass and net charge on mu-gel shrinkage and macromolecule entrapment. mu-gels formation on the surface of silicone catheters exemplifies their potential to tailor biointerfaces.Findings: Shrinkage-driven mu-gel formation strongly depends on the net charge and mass content of encapsulated macromolecules. Inclusion of neutral DEX decreases the degree of shrinkage several times, whilst charged DEXs adopt to the backbone of oppositely charged polyelectrolytes, resulting in shrinkage comparable to that of non-loaded mu-gels. Retention of CM-DEX in mu-gels is significantly higher compared to DEAE-DEX. These insights into the mechanisms of macromolecular entrapment into biopolymer-based mu-gels promotes fundamental understanding of molecular dynamics within the multilayer assemblies. Organization of biodegradable mu-gels at biomaterial surfaces opens avenues for their further exploitation in a diverse array of bioapplications.(c) 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).}},
  author       = {{Campbell, Jack and  Taghavi, Aaron and  Preis, Alexander and  Martin, Sina and Skirtach, Andre and  Franke, Jorg and  Volodkin, Dmitry and  Vikulina, Anna}},
  issn         = {{0021-9797}},
  journal      = {{JOURNAL OF COLLOID AND INTERFACE SCIENCE}},
  keywords     = {{Surface functionalization,Vaterite,Hard templating,Encapsulation,Multilayer,MULTILAYER CAPSULES,DRUG-DELIVERY,VATERITE CACO3,POLYELECTROLYTE,SIZE,MICROCAPSULES,FILMS}},
  language     = {{eng}},
  pages        = {{12--22}},
  title        = {{Spontaneous shrinkage drives macromolecule encapsulation into layer-by-layer assembled biopolymer microgels}},
  url          = {{http://doi.org/10.1016/j.jcis.2022.12.115}},
  volume       = {{635}},
  year         = {{2023}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: