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Cardiac electrophysiology studies in mice via the transjugular route : a comprehensive practical guide

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Abstract
Cardiac arrhythmias are associated with cardiovascular morbidity and mortality. Cardiac electrophysiology studies (EPS) use intra-cardiac catheter recording and stimulation for profound evaluation of the heart's electrical properties. The main clinical applica-tion is investigation and treatment of rhythm disorders. These techniques have been translated to the murine setting to open opportunities for detailed evaluation of the impact of different characteristics (including genetics) and interventions on cardiac electrophysiology and-pathology. Currently, a detailed description of the technique of murine transjugular EPS (which is the standard route of catheter introduction) is lacking. This article provides detailed information on EPS in mice via the transjugular route. This includes catheter placement, stimulation protocols, intracardiac tracing interpretation, artifact reduction, and surface ECG recording. In addition, reference values as obtained in C57BL/6N mice are presented for common electrophysiological pa-rameters. This detailed methodological description aims to increase accessibility and standardization of EPS in mice. Ultimately, also human research and patient care may benefit from translation of the knowledge obtained in preclinical models using this technique. NEW & NOTEWORTHY Electrophysiology studies (EPS) allow in-depth evaluation of cardiac electrophysiology and-pathology. These techniques have been adapted to the murine setting for (translational) studies, mainly focusing on arrhythmogenesis. Despite the frequent application of EPS via the transjugular route, a thorough description of the technique is currently lacking. This article aims to function as a comprehensive guide, also elaborating (for the first time) on nonsurgical aspects such as cathe-ter positioning, tracing artifacts, stimulation protocols, and reference values.
Keywords
Physiology (medical), Cardiology and Cardiovascular Medicine, Physiology, programmed electrical stimulation, mouse, jugular vein catheterization, electrophysiology, electrocardiography

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MLA
Favere, Kasper, et al. “Cardiac Electrophysiology Studies in Mice via the Transjugular Route : A Comprehensive Practical Guide.” AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, vol. 323, no. 4, American Physiological Society, 2022, pp. H763–73, doi:10.1152/ajpheart.00337.2022.
APA
Favere, K., Van Fraeyenhove, J., Jacobs, G., Bosman, M., Eens, S., De Sutter, J., … Heidbuchel, H. (2022). Cardiac electrophysiology studies in mice via the transjugular route : a comprehensive practical guide. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 323(4), H763–H773. https://doi.org/10.1152/ajpheart.00337.2022
Chicago author-date
Favere, Kasper, Jens Van Fraeyenhove, Griet Jacobs, Matthias Bosman, Sander Eens, Johan De Sutter, Hielko Miljoen, et al. 2022. “Cardiac Electrophysiology Studies in Mice via the Transjugular Route : A Comprehensive Practical Guide.” AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 323 (4): H763–73. https://doi.org/10.1152/ajpheart.00337.2022.
Chicago author-date (all authors)
Favere, Kasper, Jens Van Fraeyenhove, Griet Jacobs, Matthias Bosman, Sander Eens, Johan De Sutter, Hielko Miljoen, Pieter-Jan Guns, Gilles W. De Keulenaer, Vincent F. M. Segers, and Hein Heidbuchel. 2022. “Cardiac Electrophysiology Studies in Mice via the Transjugular Route : A Comprehensive Practical Guide.” AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY 323 (4): H763–H773. doi:10.1152/ajpheart.00337.2022.
Vancouver
1.
Favere K, Van Fraeyenhove J, Jacobs G, Bosman M, Eens S, De Sutter J, et al. Cardiac electrophysiology studies in mice via the transjugular route : a comprehensive practical guide. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY. 2022;323(4):H763–73.
IEEE
[1]
K. Favere et al., “Cardiac electrophysiology studies in mice via the transjugular route : a comprehensive practical guide,” AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, vol. 323, no. 4, pp. H763–H773, 2022.
@article{01GQB1SSPHHNRAFYMP4YD284Q1,
  abstract     = {{Cardiac arrhythmias are associated with cardiovascular morbidity and mortality. Cardiac electrophysiology studies (EPS) use intra-cardiac catheter recording and stimulation for profound evaluation of the heart's electrical properties. The main clinical applica-tion is investigation and treatment of rhythm disorders. These techniques have been translated to the murine setting to open opportunities for detailed evaluation of the impact of different characteristics (including genetics) and interventions on cardiac electrophysiology and-pathology. Currently, a detailed description of the technique of murine transjugular EPS (which is the standard route of catheter introduction) is lacking. This article provides detailed information on EPS in mice via the transjugular route. This includes catheter placement, stimulation protocols, intracardiac tracing interpretation, artifact reduction, and surface ECG recording. In addition, reference values as obtained in C57BL/6N mice are presented for common electrophysiological pa-rameters. This detailed methodological description aims to increase accessibility and standardization of EPS in mice. Ultimately, also human research and patient care may benefit from translation of the knowledge obtained in preclinical models using this technique. NEW & NOTEWORTHY Electrophysiology studies (EPS) allow in-depth evaluation of cardiac electrophysiology and-pathology. These techniques have been adapted to the murine setting for (translational) studies, mainly focusing on arrhythmogenesis. Despite the frequent application of EPS via the transjugular route, a thorough description of the technique is currently lacking. This article aims to function as a comprehensive guide, also elaborating (for the first time) on nonsurgical aspects such as cathe-ter positioning, tracing artifacts, stimulation protocols, and reference values.}},
  author       = {{Favere, Kasper and Van Fraeyenhove, Jens and Jacobs, Griet and Bosman, Matthias and Eens, Sander and De Sutter, Johan and Miljoen, Hielko and Guns, Pieter-Jan and De Keulenaer, Gilles W. and Segers, Vincent F. M. and Heidbuchel, Hein}},
  issn         = {{0363-6135}},
  journal      = {{AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY}},
  keywords     = {{Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology,programmed electrical stimulation,mouse,jugular vein catheterization,electrophysiology,electrocardiography}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{H763--H773}},
  publisher    = {{American Physiological Society}},
  title        = {{Cardiac electrophysiology studies in mice via the transjugular route : a comprehensive practical guide}},
  url          = {{http://doi.org/10.1152/ajpheart.00337.2022}},
  volume       = {{323}},
  year         = {{2022}},
}

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