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A transcriptional complex of FtMYB102 and FtbHLH4 coordinately regulates the accumulation of rutin in Fagopyrum tataricum

Yaolei Mi, Yu Li, Guangtao Qian, Dr. Lucas Vanhaelewyn (UGent) , Xiangxiao Meng, Tingxia Liu, Wei Yang, Yuhua Shi, Pengda Ma, Atia tul-Wahab, et al.
(2023) PLANT PHYSIOLOGY AND BIOCHEMISTRY. 194. p.696-707
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Abstract
Tartary buckwheat is rich in flavonoids, which not only play an important role in the plant-environment interaction, but are also beneficial to human health. Rutin is a therapeutic flavonol which is massively accumulated in Tartary buckwheat. It has been demonstrated that transcription factors control rutin biosynthesis. However, the transcriptional regulatory network of rutin is not fully clear. In this study, through transcriptome and target metabolomics, we validated the role of FtMYB102 and FtbHLH4 TFs at the different developmental stages of Tartary buckwheat. The elevated accumulation of rutin in the sprout appears to be closely associated with the expression of FtMYB102 and FtbHLH4. Yeast two-hybrid, transient luciferase activity and coimmunoprecipitation demonstrated that FtMYB102 and FtbHLH4 can interact and form a transcriptional complex. Moreover, yeast one-hybrid showed that both FtMYB102 and FtbHLH4 directly bind to the promoter of chalcone isomerase (CHI), and they can coordinately induce CHI expression as shown by transient luciferase activity assay. Finally, we transferred FtMYB102 and FtbHLH4 into the hairy roots of Tartary buckwheat and found that they both can promote the accumulation of rutin. Our results indicate that FtMYB102 and FtbHLH4 can form a transcriptional complex by inducing CHI expression to coordinately promote the accumulation of rutin.
Keywords
Tartary buckwheat ( Fagopyrum tataricum ), Secondary metabolism, Rutin, Transcription factors, Transcriptional regulation, Medicinal plants, PROANTHOCYANIDIN BIOSYNTHESIS, ANTHOCYANIN BIOSYNTHESIS, FLAVONOL SYNTHESIS, GENE FAMILY, ARABIDOPSIS, EXPRESSION, ENCODES, PROTEIN, MYB, IDENTIFICATION

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MLA
Mi, Yaolei, et al. “A Transcriptional Complex of FtMYB102 and FtbHLH4 Coordinately Regulates the Accumulation of Rutin in Fagopyrum Tataricum.” PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 194, 2023, pp. 696–707, doi:10.1016/j.plaphy.2022.12.016.
APA
Mi, Y., Li, Y., Qian, G., Vanhaelewyn, Dr. L., Meng, X., Liu, T., … Zhang, D. (2023). A transcriptional complex of FtMYB102 and FtbHLH4 coordinately regulates the accumulation of rutin in Fagopyrum tataricum. PLANT PHYSIOLOGY AND BIOCHEMISTRY, 194, 696–707. https://doi.org/10.1016/j.plaphy.2022.12.016
Chicago author-date
Mi, Yaolei, Yu Li, Guangtao Qian, Dr. Lucas Vanhaelewyn, Xiangxiao Meng, Tingxia Liu, Wei Yang, et al. 2023. “A Transcriptional Complex of FtMYB102 and FtbHLH4 Coordinately Regulates the Accumulation of Rutin in Fagopyrum Tataricum.” PLANT PHYSIOLOGY AND BIOCHEMISTRY 194: 696–707. https://doi.org/10.1016/j.plaphy.2022.12.016.
Chicago author-date (all authors)
Mi, Yaolei, Yu Li, Guangtao Qian, Dr. Lucas Vanhaelewyn, Xiangxiao Meng, Tingxia Liu, Wei Yang, Yuhua Shi, Pengda Ma, Atia tul-Wahab, András Viczián, Shilin Chen, Wei Sun, and Dong Zhang. 2023. “A Transcriptional Complex of FtMYB102 and FtbHLH4 Coordinately Regulates the Accumulation of Rutin in Fagopyrum Tataricum.” PLANT PHYSIOLOGY AND BIOCHEMISTRY 194: 696–707. doi:10.1016/j.plaphy.2022.12.016.
Vancouver
1.
Mi Y, Li Y, Qian G, Vanhaelewyn DrL, Meng X, Liu T, et al. A transcriptional complex of FtMYB102 and FtbHLH4 coordinately regulates the accumulation of rutin in Fagopyrum tataricum. PLANT PHYSIOLOGY AND BIOCHEMISTRY. 2023;194:696–707.
IEEE
[1]
Y. Mi et al., “A transcriptional complex of FtMYB102 and FtbHLH4 coordinately regulates the accumulation of rutin in Fagopyrum tataricum,” PLANT PHYSIOLOGY AND BIOCHEMISTRY, vol. 194, pp. 696–707, 2023.
@article{01GQ2FFYNDJ2V7K3BHKPQG1P3P,
  abstract     = {{Tartary buckwheat is rich in flavonoids, which not only play an important role in the plant-environment interaction, but are also beneficial to human health. Rutin is a therapeutic flavonol which is massively accumulated in Tartary buckwheat. It has been demonstrated that transcription factors control rutin biosynthesis. However, the transcriptional regulatory network of rutin is not fully clear. In this study, through transcriptome and target metabolomics, we validated the role of FtMYB102 and FtbHLH4 TFs at the different developmental stages of Tartary buckwheat. The elevated accumulation of rutin in the sprout appears to be closely associated with the expression of FtMYB102 and FtbHLH4. Yeast two-hybrid, transient luciferase activity and coimmunoprecipitation demonstrated that FtMYB102 and FtbHLH4 can interact and form a transcriptional complex. Moreover, yeast one-hybrid showed that both FtMYB102 and FtbHLH4 directly bind to the promoter of chalcone isomerase (CHI), and they can coordinately induce CHI expression as shown by transient luciferase activity assay. Finally, we transferred FtMYB102 and FtbHLH4 into the hairy roots of Tartary buckwheat and found that they both can promote the accumulation of rutin. Our results indicate that FtMYB102 and FtbHLH4 can form a transcriptional complex by inducing CHI expression to coordinately promote the accumulation of rutin.}},
  author       = {{Mi, Yaolei and Li, Yu and Qian, Guangtao and Vanhaelewyn, Dr. Lucas and Meng, Xiangxiao and Liu, Tingxia and Yang, Wei and Shi, Yuhua and Ma, Pengda and tul-Wahab, Atia and Viczián, András and Chen, Shilin and Sun, Wei and Zhang, Dong}},
  issn         = {{0981-9428}},
  journal      = {{PLANT PHYSIOLOGY AND BIOCHEMISTRY}},
  keywords     = {{Tartary buckwheat ( Fagopyrum tataricum ),Secondary metabolism,Rutin,Transcription factors,Transcriptional regulation,Medicinal plants,PROANTHOCYANIDIN BIOSYNTHESIS,ANTHOCYANIN BIOSYNTHESIS,FLAVONOL SYNTHESIS,GENE FAMILY,ARABIDOPSIS,EXPRESSION,ENCODES,PROTEIN,MYB,IDENTIFICATION}},
  language     = {{eng}},
  pages        = {{696--707}},
  title        = {{A transcriptional complex of FtMYB102 and FtbHLH4 coordinately regulates the accumulation of rutin in Fagopyrum tataricum}},
  url          = {{http://doi.org/10.1016/j.plaphy.2022.12.016}},
  volume       = {{194}},
  year         = {{2023}},
}
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