
Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator
- Author
- Peng Wang (UGent) , Wei Siao (UGent) , Xiuyang Zhao, Deepanksha Arora, Ren Wang (UGent) , Dominique Eeckhout (UGent) , Jelle Van Leene (UGent) , Rahul Kumar (UGent) , Anaxi Houbaert, Nancy De Winne (UGent) , Evelien Mylle (UGent) , Michaël Vandorpe (UGent) , Ruud A. Korver, Christa Testerink, Kris Gevaert (UGent) , Steffen Vanneste (UGent) , Geert De Jaeger (UGent) , Daniël Van Damme (UGent) and Eugenia Russinova (UGent)
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- Abstract
- Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargoes and coated vesicle accessory proteins. As in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargoes of overlapping vesicular routes are anticipated. The plant AP complexes are part of the sorting machinery, but despite some functional information, their cargoes, accessory proteins and regulation remain largely unknown. Here, by means of various proteomics approaches, we generated the overall interactome of the five AP and the TPLATE complexes in Arabidopsis thaliana. The interactome converged on a number of hub proteins, including the thus far unknown adaptin binding-like protein, designated P34. P34 interacted with the clathrin-associated AP complexes, controlled their stability and, subsequently, influenced clathrin-mediated endocytosis and various post-Golgi trafficking routes. Altogether, the AP interactome network offers substantial resources for further discoveries of unknown endomembrane trafficking regulators in plant cells.
- Keywords
- Plant Science
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GQ25G3V4FR7YCM69FY4V5A29
- MLA
- Wang, Peng, et al. “Adaptor Protein Complex Interaction Map in Arabidopsis Identifies P34 as a Common Stability Regulator.” NATURE PLANTS, vol. 9, Springer Science and Business Media LLC, 2023, pp. 355–71, doi:10.1038/s41477-022-01328-2.
- APA
- Wang, P., Siao, W., Zhao, X., Arora, D., Wang, R., Eeckhout, D., … Russinova, E. (2023). Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator. NATURE PLANTS, 9, 355–371. https://doi.org/10.1038/s41477-022-01328-2
- Chicago author-date
- Wang, Peng, Wei Siao, Xiuyang Zhao, Deepanksha Arora, Ren Wang, Dominique Eeckhout, Jelle Van Leene, et al. 2023. “Adaptor Protein Complex Interaction Map in Arabidopsis Identifies P34 as a Common Stability Regulator.” NATURE PLANTS 9: 355–71. https://doi.org/10.1038/s41477-022-01328-2.
- Chicago author-date (all authors)
- Wang, Peng, Wei Siao, Xiuyang Zhao, Deepanksha Arora, Ren Wang, Dominique Eeckhout, Jelle Van Leene, Rahul Kumar, Anaxi Houbaert, Nancy De Winne, Evelien Mylle, Michaël Vandorpe, Ruud A. Korver, Christa Testerink, Kris Gevaert, Steffen Vanneste, Geert De Jaeger, Daniël Van Damme, and Eugenia Russinova. 2023. “Adaptor Protein Complex Interaction Map in Arabidopsis Identifies P34 as a Common Stability Regulator.” NATURE PLANTS 9: 355–371. doi:10.1038/s41477-022-01328-2.
- Vancouver
- 1.Wang P, Siao W, Zhao X, Arora D, Wang R, Eeckhout D, et al. Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator. NATURE PLANTS. 2023;9:355–71.
- IEEE
- [1]P. Wang et al., “Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator,” NATURE PLANTS, vol. 9, pp. 355–371, 2023.
@article{01GQ25G3V4FR7YCM69FY4V5A29, abstract = {{Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargoes and coated vesicle accessory proteins. As in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargoes of overlapping vesicular routes are anticipated. The plant AP complexes are part of the sorting machinery, but despite some functional information, their cargoes, accessory proteins and regulation remain largely unknown. Here, by means of various proteomics approaches, we generated the overall interactome of the five AP and the TPLATE complexes in Arabidopsis thaliana. The interactome converged on a number of hub proteins, including the thus far unknown adaptin binding-like protein, designated P34. P34 interacted with the clathrin-associated AP complexes, controlled their stability and, subsequently, influenced clathrin-mediated endocytosis and various post-Golgi trafficking routes. Altogether, the AP interactome network offers substantial resources for further discoveries of unknown endomembrane trafficking regulators in plant cells.}}, author = {{Wang, Peng and Siao, Wei and Zhao, Xiuyang and Arora, Deepanksha and Wang, Ren and Eeckhout, Dominique and Van Leene, Jelle and Kumar, Rahul and Houbaert, Anaxi and De Winne, Nancy and Mylle, Evelien and Vandorpe, Michaël and Korver, Ruud A. and Testerink, Christa and Gevaert, Kris and Vanneste, Steffen and De Jaeger, Geert and Van Damme, Daniël and Russinova, Eugenia}}, issn = {{2055-026X}}, journal = {{NATURE PLANTS}}, keywords = {{Plant Science}}, language = {{eng}}, pages = {{355--371}}, publisher = {{Springer Science and Business Media LLC}}, title = {{Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator}}, url = {{http://doi.org/10.1038/s41477-022-01328-2}}, volume = {{9}}, year = {{2023}}, }
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