
Micro‐topographies induce epigenetic reprogramming and quiescence in human mesenchymal stem cells
- Author
- Steven Vermeulen, Bart Van Puyvelde (UGent) , Laura Bengtsson del Barrio, Ruben Almey (UGent) , Bernard K. van der Veer, Dieter Deforce (UGent) , Maarten Dhaenens (UGent) and Jan de Boer
- Organization
- Project
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- Ion mobility assisted Data Independent Acquisition of the histone code: opening up peptide-centric data mining.
- Data Independent Acquisition Mass Spectrometry to Mine Human Stem Cell Histone Epigenetics
- Epic Proteomics: an MS-based workflow for parallel profiling of histone epigenetics and protein expression.
- Abstract
- Biomaterials can control cell and nuclear morphology. Since the shape of the nucleus influences chromatin architecture, gene expression and cell identity, surface topography can control cell phenotype. This study provides fundamental insights into how surface topography influences nuclear morphology, histone modifications, and expression of histone-associated proteins through advanced histone mass spectrometry and microarray analysis. The authors find that nuclear confinement is associated with a loss of histone acetylation and nucleoli abundance, while pathway analysis reveals a substantial reduction in gene expression associated with chromosome organization. In light of previous observations where the authors found a decrease in proliferation and metabolism induced by micro-topographies, they connect these findings with a quiescent phenotype in mesenchymal stem cells, as further shown by a reduction of ribosomal proteins and the maintenance of multipotency on micro-topographies after long-term culture conditions. Also, this influence of micro-topographies on nuclear morphology and proliferation is reversible, as shown by a return of proliferation when re-cultured on a flat surface. The findings provide novel insights into how biophysical signaling influences the epigenetic landscape and subsequent cellular phenotype.
- Keywords
- biomaterials, epigenetics, mechanobiology, mesenchymal stem cells, nucleus, PRE-RIBOSOMAL-RNA, SELF-RENEWAL, HISTONE PROPIONYLATION, BIOPHYSICAL REGULATION, SURFACE TOPOGRAPHIES, NUCLEOLIN FUNCTIONS, DIFFERENTIATION, PROTEIN, ADHESION, GENE
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Advanced Science - 2022 - Vermeulen - Micro‐Topographies Induce Epigenetic Reprogramming and Quiescence in Human.pdf
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GPE06XJKTV7AAJ0J8QJAJ7FH
- MLA
- Vermeulen, Steven, et al. “Micro‐topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells.” ADVANCED SCIENCE, vol. 10, no. 1, 2023, doi:10.1002/advs.202203880.
- APA
- Vermeulen, S., Van Puyvelde, B., Bengtsson del Barrio, L., Almey, R., van der Veer, B. K., Deforce, D., … de Boer, J. (2023). Micro‐topographies induce epigenetic reprogramming and quiescence in human mesenchymal stem cells. ADVANCED SCIENCE, 10(1). https://doi.org/10.1002/advs.202203880
- Chicago author-date
- Vermeulen, Steven, Bart Van Puyvelde, Laura Bengtsson del Barrio, Ruben Almey, Bernard K. van der Veer, Dieter Deforce, Maarten Dhaenens, and Jan de Boer. 2023. “Micro‐topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells.” ADVANCED SCIENCE 10 (1). https://doi.org/10.1002/advs.202203880.
- Chicago author-date (all authors)
- Vermeulen, Steven, Bart Van Puyvelde, Laura Bengtsson del Barrio, Ruben Almey, Bernard K. van der Veer, Dieter Deforce, Maarten Dhaenens, and Jan de Boer. 2023. “Micro‐topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells.” ADVANCED SCIENCE 10 (1). doi:10.1002/advs.202203880.
- Vancouver
- 1.Vermeulen S, Van Puyvelde B, Bengtsson del Barrio L, Almey R, van der Veer BK, Deforce D, et al. Micro‐topographies induce epigenetic reprogramming and quiescence in human mesenchymal stem cells. ADVANCED SCIENCE. 2023;10(1).
- IEEE
- [1]S. Vermeulen et al., “Micro‐topographies induce epigenetic reprogramming and quiescence in human mesenchymal stem cells,” ADVANCED SCIENCE, vol. 10, no. 1, 2023.
@article{01GPE06XJKTV7AAJ0J8QJAJ7FH, abstract = {{Biomaterials can control cell and nuclear morphology. Since the shape of the nucleus influences chromatin architecture, gene expression and cell identity, surface topography can control cell phenotype. This study provides fundamental insights into how surface topography influences nuclear morphology, histone modifications, and expression of histone-associated proteins through advanced histone mass spectrometry and microarray analysis. The authors find that nuclear confinement is associated with a loss of histone acetylation and nucleoli abundance, while pathway analysis reveals a substantial reduction in gene expression associated with chromosome organization. In light of previous observations where the authors found a decrease in proliferation and metabolism induced by micro-topographies, they connect these findings with a quiescent phenotype in mesenchymal stem cells, as further shown by a reduction of ribosomal proteins and the maintenance of multipotency on micro-topographies after long-term culture conditions. Also, this influence of micro-topographies on nuclear morphology and proliferation is reversible, as shown by a return of proliferation when re-cultured on a flat surface. The findings provide novel insights into how biophysical signaling influences the epigenetic landscape and subsequent cellular phenotype.}}, articleno = {{2203880}}, author = {{Vermeulen, Steven and Van Puyvelde, Bart and Bengtsson del Barrio, Laura and Almey, Ruben and van der Veer, Bernard K. and Deforce, Dieter and Dhaenens, Maarten and de Boer, Jan}}, issn = {{2198-3844}}, journal = {{ADVANCED SCIENCE}}, keywords = {{biomaterials,epigenetics,mechanobiology,mesenchymal stem cells,nucleus,PRE-RIBOSOMAL-RNA,SELF-RENEWAL,HISTONE PROPIONYLATION,BIOPHYSICAL REGULATION,SURFACE TOPOGRAPHIES,NUCLEOLIN FUNCTIONS,DIFFERENTIATION,PROTEIN,ADHESION,GENE}}, language = {{eng}}, number = {{1}}, pages = {{15}}, title = {{Micro‐topographies induce epigenetic reprogramming and quiescence in human mesenchymal stem cells}}, url = {{http://doi.org/10.1002/advs.202203880}}, volume = {{10}}, year = {{2023}}, }
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