@article{01GPDBNKV77HFKY5JXXBR82REV,
  abstract     = {{Organoid cultures could constitute a valuable in vitro model to explore new treatments for canine (c) medullary thyroid carcinoma (MTC). The study's objectives were to estab-lish and characterize 3D organoid cultures of cMTC using histology and immunohisto-chemistry (IHC) and to evaluate the effect of antitumor drugs on organoids' viability. Five cMTC tissue samples were used to develop organoid cultures of which one orga-noid line, named cMTC N degrees 2, could be passaged for an extended period. This cMTC N degrees 2 organoid line was further compared to the primary tumour regarding morphology and IHC expression of thyroid transcription factor-1 (TTF-1), thyroglobulin, calcitonin, synaptophysin, vimentin, Ki-67, cyclooxygenase-2 (COX-2), P-glycoprotein and vascular endothelial growth factor (VEGF). Quality control of the cMTC N degrees 2 organoid line was achieved by a single nucleotide polymorphism (SNP) array of the organoids, primary tumour and healthy blood cells of the same dog. The effect of carboplatin, meloxicam and toceranib phosphate (TOC) on cMTC N degrees 2 organoids' viability was evaluated. The cMTC N degrees 2 organoid line was cultured for 94 days and showed similar histological fea-tures with the primary tumour. Immunolabelling for TTF-1, thyroglobulin, calcitonin and VEGF was similar between the primary tumour and cMTC N degrees 2 organoids. Compared to the primary tumour, organoids showed higher immunolabelling for vimentin and Ki-67, and lower immunolabelling for synaptophysin, COX-2 and P-glycoprotein. The SNP genotype was similar for each chromosome between healthy blood cells, primary tumour and cMTC N degrees 2 organoids. Carboplatin, meloxicam and TOC had no effect on cMTC N degrees 2 organoid cell viability within achievable in vivo concentration range. In conclusion, the cMTC N degrees 2 organoid line is a promising first milestone towards an established in vitro organoid model to explore pathophysiology and new treatment modalities in cMTC.}},
  author       = {{Scheemaeker, Stephanie and Inglebert, Marine and Daminet, Sylvie and Dettwiler, Martina and Letko, Anna and Drögemüller, Cord and Kessler, Martin and Ducatelle, Richard and Rottenberg, Sven and Campos, Miguel}},
  issn         = {{1476-5810}},
  journal      = {{VETERINARY AND COMPARATIVE ONCOLOGY}},
  keywords     = {{General Veterinary,cell culture techniques,dogs,histology,immunohistochemistry,neoplasms,thyroid carcinoma,TOCERANIB PHOSPHATE,CANCER,ESTABLISHMENT,RESISTANCE,INHIBITOR,PROTEIN,CELLS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{111--122}},
  title        = {{Organoids of patient-derived medullary thyroid carcinoma : the first milestone towards a new in vitro model in dogs}},
  url          = {{http://doi.org/10.1111/vco.12872}},
  volume       = {{21}},
  year         = {{2023}},
}

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