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The effects of combined exposure to simulated microgravity, ionizing radiation, and cortisol on the in vitro wound healing process

Wilhelmina Radstake, Kiran Gautam, Silvana Ferreira Da Silva Miranda (UGent) , Randy Vermeesen, Kevin Tabury, Emil Rehnberg (UGent) , Jasmine Buset, Ann Janssen, Liselotte Leysen, Mieke Neefs, et al.
(2023) CELLS. 12(2).
Author
Organization
Abstract
Human spaceflight is associated with several health-related issues as a result of long-term exposure to microgravity, ionizing radiation, and higher levels of psychological stress. Frequent reported skin problems in space include rashes, itches, and a delayed wound healing. Access to space is restricted by financial and logistical issues; as a consequence, experimental sample sizes are often small, which limits the generalization of the results. Earth-based simulation models can be used to investigate cellular responses as a result of exposure to certain spaceflight stressors. Here, we describe the development of an in vitro model of the simulated spaceflight environment, which we used to investigate the combined effect of simulated microgravity using the random positioning machine (RPM), ionizing radiation, and stress hormones on the wound-healing capacity of human dermal fibroblasts. Fibroblasts were exposed to cortisol, after which they were irradiated with different radiation qualities (including X-rays, protons, carbon ions, and iron ions) followed by exposure to simulated microgravity using a random positioning machine (RPM). Data related to the inflammatory, proliferation, and remodeling phase of wound healing has been collected. Results show that spaceflight stressors can interfere with the wound healing process at any phase. Moreover, several interactions between the different spaceflight stressors were found. This highlights the complexity that needs to be taken into account when studying the effect of spaceflight stressors on certain biological processes and for the aim of countermeasures development.
Keywords
General Medicine, radiation, microgravity, DNA damage, wound healing, in vitro, fibroblast, cortisol, protons, carbon ions, iron ions, ionizing radiation, simulated microgravity

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Citation

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MLA
Radstake, Wilhelmina, et al. “The Effects of Combined Exposure to Simulated Microgravity, Ionizing Radiation, and Cortisol on the in Vitro Wound Healing Process.” CELLS, vol. 12, no. 2, MDPI AG, 2023, doi:10.3390/cells12020246.
APA
Radstake, W., Gautam, K., Ferreira Da Silva Miranda, S., Vermeesen, R., Tabury, K., Rehnberg, E., … Baatout, S. (2023). The effects of combined exposure to simulated microgravity, ionizing radiation, and cortisol on the in vitro wound healing process. CELLS, 12(2). https://doi.org/10.3390/cells12020246
Chicago author-date
Radstake, Wilhelmina, Kiran Gautam, Silvana Ferreira Da Silva Miranda, Randy Vermeesen, Kevin Tabury, Emil Rehnberg, Jasmine Buset, et al. 2023. “The Effects of Combined Exposure to Simulated Microgravity, Ionizing Radiation, and Cortisol on the in Vitro Wound Healing Process.” CELLS 12 (2). https://doi.org/10.3390/cells12020246.
Chicago author-date (all authors)
Radstake, Wilhelmina, Kiran Gautam, Silvana Ferreira Da Silva Miranda, Randy Vermeesen, Kevin Tabury, Emil Rehnberg, Jasmine Buset, Ann Janssen, Liselotte Leysen, Mieke Neefs, Mieke Verslegers, Jürgen Claesen, Marc-Jan van van Goethem, Uli Weber, Claudia Fournier, Alessio Parisi, Sytze Brandenburg, Marco Durante, Bjorn Baselet, and Sarah Baatout. 2023. “The Effects of Combined Exposure to Simulated Microgravity, Ionizing Radiation, and Cortisol on the in Vitro Wound Healing Process.” CELLS 12 (2). doi:10.3390/cells12020246.
Vancouver
1.
Radstake W, Gautam K, Ferreira Da Silva Miranda S, Vermeesen R, Tabury K, Rehnberg E, et al. The effects of combined exposure to simulated microgravity, ionizing radiation, and cortisol on the in vitro wound healing process. CELLS. 2023;12(2).
IEEE
[1]
W. Radstake et al., “The effects of combined exposure to simulated microgravity, ionizing radiation, and cortisol on the in vitro wound healing process,” CELLS, vol. 12, no. 2, 2023.
@article{01GPAZY5JXZC1SCX81NT6RAVPB,
  abstract     = {{Human spaceflight is associated with several health-related issues as a result of long-term exposure to microgravity, ionizing radiation, and higher levels of psychological stress. Frequent reported skin problems in space include rashes, itches, and a delayed wound healing. Access to space is restricted by financial and logistical issues; as a consequence, experimental sample sizes are often small, which limits the generalization of the results. Earth-based simulation models can be used to investigate cellular responses as a result of exposure to certain spaceflight stressors. Here, we describe the development of an in vitro model of the simulated spaceflight environment, which we used to investigate the combined effect of simulated microgravity using the random positioning machine (RPM), ionizing radiation, and stress hormones on the wound-healing capacity of human dermal fibroblasts. Fibroblasts were exposed to cortisol, after which they were irradiated with different radiation qualities (including X-rays, protons, carbon ions, and iron ions) followed by exposure to simulated microgravity using a random positioning machine (RPM). Data related to the inflammatory, proliferation, and remodeling phase of wound healing has been collected. Results show that spaceflight stressors can interfere with the wound healing process at any phase. Moreover, several interactions between the different spaceflight stressors were found. This highlights the complexity that needs to be taken into account when studying the effect of spaceflight stressors on certain biological processes and for the aim of countermeasures development.}},
  articleno    = {{246}},
  author       = {{Radstake, Wilhelmina and Gautam, Kiran and Ferreira Da Silva Miranda, Silvana and Vermeesen, Randy and Tabury, Kevin and Rehnberg, Emil and Buset, Jasmine and Janssen, Ann and Leysen, Liselotte and Neefs, Mieke and Verslegers, Mieke and Claesen, Jürgen and van Goethem, Marc-Jan van and Weber, Uli and Fournier, Claudia and Parisi, Alessio and Brandenburg, Sytze and Durante, Marco and Baselet, Bjorn and Baatout, Sarah}},
  issn         = {{2073-4409}},
  journal      = {{CELLS}},
  keywords     = {{General Medicine,radiation,microgravity,DNA damage,wound healing,in vitro,fibroblast,cortisol,protons,carbon ions,iron ions,ionizing radiation,simulated microgravity}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{32}},
  publisher    = {{MDPI AG}},
  title        = {{The effects of combined exposure to simulated microgravity, ionizing radiation, and cortisol on the in vitro wound healing process}},
  url          = {{http://doi.org/10.3390/cells12020246}},
  volume       = {{12}},
  year         = {{2023}},
}
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