Spectrally tunable Förster resonance energy transfer-based biosensors using organic dye grafting
- Author
- Marco Dalla Vecchia (UGent) , Amandine Conte-Daban, Benjamin Cappe (UGent) , Wim Vandenberg, Peter Vandenabeele (UGent) , Franck Riquet (UGent) and Peter Dedecker
- Organization
- Project
-
- Spatio-temporal aspects and function of MLKL at plasma membrane during necroptosis.
- MOlecular mechanisms of cellular DEath and Life decisions in Inflammation, Degeneration and Infection
- Cell death activity regulation in inflammation and cancer
- Factors that determine RIPK1-dependent necroptosis
- Investigating the role of ferroptosis in acute liver injury and multiple sclerosis with newly developed chemical tool compounds
- Mechanisms of ferroptosis mediated immunoregulation
- Autophagy in inflammation and inflammatory disorders (ATLANTIS), from basic insights to experimental therapy
- Cell Death Regulation and Role in Infection and Inflammatory Diseases
- Thymic reprogramming: The role of Death Receptor 3 (DR3)
- Abstract
- Biosensors based on Fo''rster resonance energy transfer (FRET) have revolutionized cellular biology by allowing the direct measurement of biochemical processes in situ. Many genetically encoded sensors make use of fluorescent proteins that are limited in spectral versatility and that allow few ways to change the spectral properties once the construct has been created. In this work, we developed genetically encoded FRET biosensors based on the chemigenetic SNAP and HaloTag domains combined with matching organic fluorophores. We found that the resulting constructs can display comparable responses, kinetics, and reversibility compared to their fluorescent protein based ancestors, but with the added advantage of spectral versatility, including the availability of red-shifted dye pairs. However, we also find that the introduction of these tags can alter the sensor readout, showing that careful validation is required before applying such constructs in practice. Overall, our approach delivers an innovative methodology that can readily expand the spectral variety and versatility of FRET-based biosensors.
- Keywords
- fluorescent biosensors, fluorescence imaging, Förster resonance energy transfer, chemigenetic labeling, genetically encoded labels, LIVE-CELL, FLUORESCENT PROTEINS, CHROMOPHORE FORMATION, GENERAL-METHOD, FLUOROPHORES, CA2+, TAG, PROBES
Downloads
-
(...).pdf
- full text (Published version)
- |
- UGent only
- |
- |
- 13.65 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GP3GH7S8R87BGBAQXXNN29EJ
- MLA
- Dalla Vecchia, Marco, et al. “Spectrally Tunable Förster Resonance Energy Transfer-Based Biosensors Using Organic Dye Grafting.” ACS SENSORS, vol. 7, no. 10, 2022, pp. 2920–27, doi:10.1021/acssensors.2c00066.
- APA
- Dalla Vecchia, M., Conte-Daban, A., Cappe, B., Vandenberg, W., Vandenabeele, P., Riquet, F., & Dedecker, P. (2022). Spectrally tunable Förster resonance energy transfer-based biosensors using organic dye grafting. ACS SENSORS, 7(10), 2920–2927. https://doi.org/10.1021/acssensors.2c00066
- Chicago author-date
- Dalla Vecchia, Marco, Amandine Conte-Daban, Benjamin Cappe, Wim Vandenberg, Peter Vandenabeele, Franck Riquet, and Peter Dedecker. 2022. “Spectrally Tunable Förster Resonance Energy Transfer-Based Biosensors Using Organic Dye Grafting.” ACS SENSORS 7 (10): 2920–27. https://doi.org/10.1021/acssensors.2c00066.
- Chicago author-date (all authors)
- Dalla Vecchia, Marco, Amandine Conte-Daban, Benjamin Cappe, Wim Vandenberg, Peter Vandenabeele, Franck Riquet, and Peter Dedecker. 2022. “Spectrally Tunable Förster Resonance Energy Transfer-Based Biosensors Using Organic Dye Grafting.” ACS SENSORS 7 (10): 2920–2927. doi:10.1021/acssensors.2c00066.
- Vancouver
- 1.Dalla Vecchia M, Conte-Daban A, Cappe B, Vandenberg W, Vandenabeele P, Riquet F, et al. Spectrally tunable Förster resonance energy transfer-based biosensors using organic dye grafting. ACS SENSORS. 2022;7(10):2920–7.
- IEEE
- [1]M. Dalla Vecchia et al., “Spectrally tunable Förster resonance energy transfer-based biosensors using organic dye grafting,” ACS SENSORS, vol. 7, no. 10, pp. 2920–2927, 2022.
@article{01GP3GH7S8R87BGBAQXXNN29EJ,
abstract = {{Biosensors based on Fo''rster resonance energy transfer (FRET) have revolutionized cellular biology by allowing the direct measurement of biochemical processes in situ. Many genetically encoded sensors make use of fluorescent proteins that are limited in spectral versatility and that allow few ways to change the spectral properties once the construct has been created. In this work, we developed genetically encoded FRET biosensors based on the chemigenetic SNAP and HaloTag domains combined with matching organic fluorophores. We found that the resulting constructs can display comparable responses, kinetics, and reversibility compared to their fluorescent protein based ancestors, but with the added advantage of spectral versatility, including the availability of red-shifted dye pairs. However, we also find that the introduction of these tags can alter the sensor readout, showing that careful validation is required before applying such constructs in practice. Overall, our approach delivers an innovative methodology that can readily expand the spectral variety and versatility of FRET-based biosensors.}},
author = {{Dalla Vecchia, Marco and Conte-Daban, Amandine and Cappe, Benjamin and Vandenberg, Wim and Vandenabeele, Peter and Riquet, Franck and Dedecker, Peter}},
issn = {{2379-3694}},
journal = {{ACS SENSORS}},
keywords = {{fluorescent biosensors,fluorescence imaging,Förster resonance energy transfer,chemigenetic labeling,genetically encoded labels,LIVE-CELL,FLUORESCENT PROTEINS,CHROMOPHORE FORMATION,GENERAL-METHOD,FLUOROPHORES,CA2+,TAG,PROBES}},
language = {{eng}},
number = {{10}},
pages = {{2920--2927}},
title = {{Spectrally tunable Förster resonance energy transfer-based biosensors using organic dye grafting}},
url = {{http://dx.doi.org/10.1021/acssensors.2c00066}},
volume = {{7}},
year = {{2022}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: