The CIPK23 protein kinase represses SLAC1‐type anion channels in Arabidopsis guard cells and stimulates stomatal opening
- Author
- Shouguang Huang, Tobias Maierhofer, Kenji Hashimoto, Xiangyu Xu (UGent) , Sohail M. Karimi, Heike Müller, Michael A. Geringer, Yi Wang, Jörg Kudla, Ive De Smet (UGent) , Rainer Hedrich, Dietmar Geiger and M. Rob G. Roelfsema
- Organization
- Abstract
- Guard cells control the opening of stomatal pores in the leaf surface, with the use of a network of protein kinases and phosphatases. Loss of function of the CBL-interacting protein kinase 23 (CIPK23) was previously shown to decrease the stomatal conductance, but the molecular mechanisms underlying this response still need to be clarified. CIPK23 was specifically expressed in Arabidopsis guard cells, using an estrogen-inducible system. Stomatal movements were linked to changes in ion channel activity, determined with double-barreled intracellular electrodes in guard cells and with the two-electrode voltage clamp technique in Xenopus oocytes. Expression of the phosphomimetic variant CIPK23(T190D) enhanced stomatal opening, while the natural CIPK23 and a kinase-inactive CIPK23(K60N) variant did not affect stomatal movements. Overexpression of CIPK23(T190D) repressed the activity of S-type anion channels, while their steady-state activity was unchanged by CIPK23 and CIPK23(K60N). We suggest that CIPK23 enhances the stomatal conductance at favorable growth conditions, via the regulation of several ion transport proteins in guard cells. The inhibition of SLAC1-type anion channels is an important facet of this response.
- Keywords
- AKT1, Arabidopsis thaliana, CIPK23, K+ uptake channel, SLAC1-type anion channel, SLAH3, guard cells, stomatal conductance, B-LIKE PROTEINS, ABSCISIC-ACID, K+ CHANNEL, POTASSIUM CHANNEL, CALCIUM, HOMEOSTASIS, SENSOR, EXPRESSION, TRANSPORT
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New Phytologist (2023) 238, 270–282.pdf
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GP0PYTV8TMRJ3ZS3P9PDWJ30
- MLA
- Huang, Shouguang, et al. “The CIPK23 Protein Kinase Represses SLAC1‐type Anion Channels in Arabidopsis Guard Cells and Stimulates Stomatal Opening.” NEW PHYTOLOGIST, vol. 238, no. 1, 2023, pp. 270–82, doi:10.1111/nph.18708.
- APA
- Huang, S., Maierhofer, T., Hashimoto, K., Xu, X., Karimi, S. M., Müller, H., … Roelfsema, M. R. G. (2023). The CIPK23 protein kinase represses SLAC1‐type anion channels in Arabidopsis guard cells and stimulates stomatal opening. NEW PHYTOLOGIST, 238(1), 270–282. https://doi.org/10.1111/nph.18708
- Chicago author-date
- Huang, Shouguang, Tobias Maierhofer, Kenji Hashimoto, Xiangyu Xu, Sohail M. Karimi, Heike Müller, Michael A. Geringer, et al. 2023. “The CIPK23 Protein Kinase Represses SLAC1‐type Anion Channels in Arabidopsis Guard Cells and Stimulates Stomatal Opening.” NEW PHYTOLOGIST 238 (1): 270–82. https://doi.org/10.1111/nph.18708.
- Chicago author-date (all authors)
- Huang, Shouguang, Tobias Maierhofer, Kenji Hashimoto, Xiangyu Xu, Sohail M. Karimi, Heike Müller, Michael A. Geringer, Yi Wang, Jörg Kudla, Ive De Smet, Rainer Hedrich, Dietmar Geiger, and M. Rob G. Roelfsema. 2023. “The CIPK23 Protein Kinase Represses SLAC1‐type Anion Channels in Arabidopsis Guard Cells and Stimulates Stomatal Opening.” NEW PHYTOLOGIST 238 (1): 270–282. doi:10.1111/nph.18708.
- Vancouver
- 1.Huang S, Maierhofer T, Hashimoto K, Xu X, Karimi SM, Müller H, et al. The CIPK23 protein kinase represses SLAC1‐type anion channels in Arabidopsis guard cells and stimulates stomatal opening. NEW PHYTOLOGIST. 2023;238(1):270–82.
- IEEE
- [1]S. Huang et al., “The CIPK23 protein kinase represses SLAC1‐type anion channels in Arabidopsis guard cells and stimulates stomatal opening,” NEW PHYTOLOGIST, vol. 238, no. 1, pp. 270–282, 2023.
@article{01GP0PYTV8TMRJ3ZS3P9PDWJ30,
abstract = {{Guard cells control the opening of stomatal pores in the leaf surface, with the use of a network of protein kinases and phosphatases. Loss of function of the CBL-interacting protein kinase 23 (CIPK23) was previously shown to decrease the stomatal conductance, but the molecular mechanisms underlying this response still need to be clarified. CIPK23 was specifically expressed in Arabidopsis guard cells, using an estrogen-inducible system. Stomatal movements were linked to changes in ion channel activity, determined with double-barreled intracellular electrodes in guard cells and with the two-electrode voltage clamp technique in Xenopus oocytes. Expression of the phosphomimetic variant CIPK23(T190D) enhanced stomatal opening, while the natural CIPK23 and a kinase-inactive CIPK23(K60N) variant did not affect stomatal movements. Overexpression of CIPK23(T190D) repressed the activity of S-type anion channels, while their steady-state activity was unchanged by CIPK23 and CIPK23(K60N). We suggest that CIPK23 enhances the stomatal conductance at favorable growth conditions, via the regulation of several ion transport proteins in guard cells. The inhibition of SLAC1-type anion channels is an important facet of this response.}},
author = {{Huang, Shouguang and Maierhofer, Tobias and Hashimoto, Kenji and Xu, Xiangyu and Karimi, Sohail M. and Müller, Heike and Geringer, Michael A. and Wang, Yi and Kudla, Jörg and De Smet, Ive and Hedrich, Rainer and Geiger, Dietmar and Roelfsema, M. Rob G.}},
issn = {{0028-646X}},
journal = {{NEW PHYTOLOGIST}},
keywords = {{AKT1,Arabidopsis thaliana,CIPK23,K+ uptake channel,SLAC1-type anion channel,SLAH3,guard cells,stomatal conductance,B-LIKE PROTEINS,ABSCISIC-ACID,K+ CHANNEL,POTASSIUM CHANNEL,CALCIUM,HOMEOSTASIS,SENSOR,EXPRESSION,TRANSPORT}},
language = {{eng}},
number = {{1}},
pages = {{270--282}},
title = {{The CIPK23 protein kinase represses SLAC1‐type anion channels in Arabidopsis guard cells and stimulates stomatal opening}},
url = {{http://doi.org/10.1111/nph.18708}},
volume = {{238}},
year = {{2023}},
}
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