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High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers

Ruochen Ouyang, Ana Rita Costa, C. Keith Cassidy, Aleksandra Otwinowska, Vera C. J. Williams, Agnieszka Łątka (UGent) , Phill J. Stansfeld, Zuzanna Drulis-Kawa, Yves Briers (UGent) , Daniel M. Pelt, et al.
(2022) NATURE COMMUNICATIONS. 13(1).
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Abstract
The Klebsiella jumbo myophage phi Kp24 displays an unusually complex arrangement of tail fibers interacting with a host cell. In this study, we combine cryo-electron microscopy methods, protein structure prediction methods, molecular simulations, microbiological and machine learning approaches to explore the capsid, tail, and tail fibers of phi Kp24. We determine the structure of the capsid and tail at 4.1 angstrom and 3.0 angstrom resolution. We observe the tail fibers are branched and rearranged dramatically upon cell surface attachment. This complex configuration involves fourteen putative tail fibers with depolymerase activity that provide phi Kp24 with the ability to infect a broad panel of capsular polysaccharide (CPS) types of Klebsiella pneumoniae. Our study provides structural and functional insight into how phi Kp24 adapts to the variable surfaces of capsulated bacterial pathogens, which is useful for the development of phage therapy approaches against pan-drug resistant K. pneumoniae strains.
Keywords
CRYO-EM STRUCTURE, 3-DIMENSIONAL STRUCTURE, MOLECULAR-DYNAMICS, PROTEIN, STRUCTURES, PREDICTION, IMPLEMENTATION, VISUALIZATION, TAILSPIKE, APPARATUS, SHEATH

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Citation

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MLA
Ouyang, Ruochen, et al. “High-Resolution Reconstruction of a Jumbo-Bacteriophage Infecting Capsulated Bacteria Using Hyperbranched Tail Fibers.” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022, doi:10.1038/s41467-022-34972-5.
APA
Ouyang, R., Costa, A. R., Cassidy, C. K., Otwinowska, A., Williams, V. C. J., Łątka, A., … Briegel, A. (2022). High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers. NATURE COMMUNICATIONS, 13(1). https://doi.org/10.1038/s41467-022-34972-5
Chicago author-date
Ouyang, Ruochen, Ana Rita Costa, C. Keith Cassidy, Aleksandra Otwinowska, Vera C. J. Williams, Agnieszka Łątka, Phill J. Stansfeld, et al. 2022. “High-Resolution Reconstruction of a Jumbo-Bacteriophage Infecting Capsulated Bacteria Using Hyperbranched Tail Fibers.” NATURE COMMUNICATIONS 13 (1). https://doi.org/10.1038/s41467-022-34972-5.
Chicago author-date (all authors)
Ouyang, Ruochen, Ana Rita Costa, C. Keith Cassidy, Aleksandra Otwinowska, Vera C. J. Williams, Agnieszka Łątka, Phill J. Stansfeld, Zuzanna Drulis-Kawa, Yves Briers, Daniel M. Pelt, Stan J. J. Brouns, and Ariane Briegel. 2022. “High-Resolution Reconstruction of a Jumbo-Bacteriophage Infecting Capsulated Bacteria Using Hyperbranched Tail Fibers.” NATURE COMMUNICATIONS 13 (1). doi:10.1038/s41467-022-34972-5.
Vancouver
1.
Ouyang R, Costa AR, Cassidy CK, Otwinowska A, Williams VCJ, Łątka A, et al. High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers. NATURE COMMUNICATIONS. 2022;13(1).
IEEE
[1]
R. Ouyang et al., “High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers,” NATURE COMMUNICATIONS, vol. 13, no. 1, 2022.
@article{01GMWG4SVQ1BYA1RCXYQNT9E66,
  abstract     = {{The Klebsiella jumbo myophage phi Kp24 displays an unusually complex arrangement of tail fibers interacting with a host cell. In this study, we combine cryo-electron microscopy methods, protein structure prediction methods, molecular simulations, microbiological and machine learning approaches to explore the capsid, tail, and tail fibers of phi Kp24. We determine the structure of the capsid and tail at 4.1 angstrom and 3.0 angstrom resolution. We observe the tail fibers are branched and rearranged dramatically upon cell surface attachment. This complex configuration involves fourteen putative tail fibers with depolymerase activity that provide phi Kp24 with the ability to infect a broad panel of capsular polysaccharide (CPS) types of Klebsiella pneumoniae. Our study provides structural and functional insight into how phi Kp24 adapts to the variable surfaces of capsulated bacterial pathogens, which is useful for the development of phage therapy approaches against pan-drug resistant K. pneumoniae strains.}},
  articleno    = {{7241}},
  author       = {{Ouyang, Ruochen and  Costa, Ana Rita and  Cassidy, C. Keith and  Otwinowska, Aleksandra and  Williams, Vera C. J. and Łątka, Agnieszka and  Stansfeld, Phill J. and  Drulis-Kawa, Zuzanna and Briers, Yves and  Pelt, Daniel M. and  Brouns, Stan J. J. and  Briegel, Ariane}},
  issn         = {{2041-1723}},
  journal      = {{NATURE COMMUNICATIONS}},
  keywords     = {{CRYO-EM STRUCTURE,3-DIMENSIONAL STRUCTURE,MOLECULAR-DYNAMICS,PROTEIN,STRUCTURES,PREDICTION,IMPLEMENTATION,VISUALIZATION,TAILSPIKE,APPARATUS,SHEATH}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{16}},
  title        = {{High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers}},
  url          = {{http://doi.org/10.1038/s41467-022-34972-5}},
  volume       = {{13}},
  year         = {{2022}},
}
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