Deciphering Alternaria metabolic responses in microbial confrontation via an integrated mass spectrometric targeted and non-targeted strategy
- Author
- Ye Tian, Mohamed Fathi Abdallah (UGent) , Marthe De Boevre (UGent) , Cheng Wang, Kris Audenaert (UGent) , Sarah De Saeger (UGent) and Aibo Wu
- Organization
- Project
- Abstract
- Microbial confrontation is ubiquitously present in nature, such as mycoparasitism, competition and antibiosis between biocontrol agents and microbial pathogens. However, the internal metabolic responses of fungal pathogens under microbial interaction scenario have been scarcely investigated. In this study, we set up an integrated mycotoxin analysis and non-targeted metabolomics workflow to decipher metabolic changes of Alternaria pathogens when confronted with selected Trichoderma strains, as well as mycotoxin metabolization in the Trichoderma spp. Results demonstrated that Trichoderma spp. significantly influenced mycotoxin production and whole metabolome of Alternaria pathogens when in cocultivation, and one Trichoderma strain could metabolize alternariol into its hydroxylated form. These differential metabolites revealed fungal physiological alternations in various confrontation conditions. In all, a MS-based strategy was proposed to investigate mi-crobial metabolic profiles under fungal/fungal and fungal/mycotoxin cocultivation, and this generic method-ology would be significant for understanding the occurrence and change of food contaminants in microbial interactions.
- Keywords
- FOOD-PRODUCTS, MYCOTOXINS, TOXINS, Microbial confrontation, Metabolic profiling, Alternaria, Trichoderma, Mycotoxin
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01GM39JSN3Z3X5ADDYNNSWS1HD
- MLA
- Tian, Ye, et al. “Deciphering Alternaria Metabolic Responses in Microbial Confrontation via an Integrated Mass Spectrometric Targeted and Non-Targeted Strategy.” FOOD CHEMISTRY, vol. 404, no. part B, 2023, doi:10.1016/j.foodchem.2022.134694.
- APA
- Tian, Y., Abdallah, M. F., De Boevre, M., Wang, C., Audenaert, K., De Saeger, S., & Wu, A. (2023). Deciphering Alternaria metabolic responses in microbial confrontation via an integrated mass spectrometric targeted and non-targeted strategy. FOOD CHEMISTRY, 404(part B). https://doi.org/10.1016/j.foodchem.2022.134694
- Chicago author-date
- Tian, Ye, Mohamed Fathi Abdallah, Marthe De Boevre, Cheng Wang, Kris Audenaert, Sarah De Saeger, and Aibo Wu. 2023. “Deciphering Alternaria Metabolic Responses in Microbial Confrontation via an Integrated Mass Spectrometric Targeted and Non-Targeted Strategy.” FOOD CHEMISTRY 404 (part B). https://doi.org/10.1016/j.foodchem.2022.134694.
- Chicago author-date (all authors)
- Tian, Ye, Mohamed Fathi Abdallah, Marthe De Boevre, Cheng Wang, Kris Audenaert, Sarah De Saeger, and Aibo Wu. 2023. “Deciphering Alternaria Metabolic Responses in Microbial Confrontation via an Integrated Mass Spectrometric Targeted and Non-Targeted Strategy.” FOOD CHEMISTRY 404 (part B). doi:10.1016/j.foodchem.2022.134694.
- Vancouver
- 1.Tian Y, Abdallah MF, De Boevre M, Wang C, Audenaert K, De Saeger S, et al. Deciphering Alternaria metabolic responses in microbial confrontation via an integrated mass spectrometric targeted and non-targeted strategy. FOOD CHEMISTRY. 2023;404(part B).
- IEEE
- [1]Y. Tian et al., “Deciphering Alternaria metabolic responses in microbial confrontation via an integrated mass spectrometric targeted and non-targeted strategy,” FOOD CHEMISTRY, vol. 404, no. part B, 2023.
@article{01GM39JSN3Z3X5ADDYNNSWS1HD,
abstract = {{Microbial confrontation is ubiquitously present in nature, such as mycoparasitism, competition and antibiosis between biocontrol agents and microbial pathogens. However, the internal metabolic responses of fungal pathogens under microbial interaction scenario have been scarcely investigated. In this study, we set up an integrated mycotoxin analysis and non-targeted metabolomics workflow to decipher metabolic changes of Alternaria pathogens when confronted with selected Trichoderma strains, as well as mycotoxin metabolization in the Trichoderma spp. Results demonstrated that Trichoderma spp. significantly influenced mycotoxin production and whole metabolome of Alternaria pathogens when in cocultivation, and one Trichoderma strain could metabolize alternariol into its hydroxylated form. These differential metabolites revealed fungal physiological alternations in various confrontation conditions. In all, a MS-based strategy was proposed to investigate mi-crobial metabolic profiles under fungal/fungal and fungal/mycotoxin cocultivation, and this generic method-ology would be significant for understanding the occurrence and change of food contaminants in microbial interactions.}},
articleno = {{134694}},
author = {{Tian, Ye and Abdallah, Mohamed Fathi and De Boevre, Marthe and Wang, Cheng and Audenaert, Kris and De Saeger, Sarah and Wu, Aibo}},
issn = {{0308-8146}},
journal = {{FOOD CHEMISTRY}},
keywords = {{FOOD-PRODUCTS,MYCOTOXINS,TOXINS,Microbial confrontation,Metabolic profiling,Alternaria,Trichoderma,Mycotoxin}},
language = {{eng}},
number = {{part B}},
pages = {{10}},
title = {{Deciphering Alternaria metabolic responses in microbial confrontation via an integrated mass spectrometric targeted and non-targeted strategy}},
url = {{http://doi.org/10.1016/j.foodchem.2022.134694}},
volume = {{404}},
year = {{2023}},
}
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