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Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins

Jelle Van Leene (UGent) , Dominique Eeckhout (UGent) , Astrid Gadeyne (UGent) , Caroline Matthijs, Chao Han (UGent) , Nancy De Winne (UGent) , Geert Persiau (UGent) , Eveline Van De Slijke (UGent) , Freya Persyn (UGent) , Toon Mertens (UGent) , et al.
(2022) NATURE PLANTS. 8(11). p.1245-1261
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Abstract
The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited. Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking. Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation. At the intersection of this multi-dimensional interactome, we discovered a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins. Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1. Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
Keywords
Plant Science, TREHALOSE 6-PHOSPHATE, ARABIDOPSIS-THALIANA, NEGATIVE REGULATORS, METABOLIC PATHWAYS, SENSOR SNRK1, PHOSPHORYLATION, COMPLEXES, EVOLUTION, STARCH, LOCALIZATION

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Citation

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MLA
Van Leene, Jelle, et al. “Mapping of the Plant SnRK1 Kinase Signalling Network Reveals a Key Regulatory Role for the Class II T6P Synthase-like Proteins.” NATURE PLANTS, vol. 8, no. 11, 2022, pp. 1245–61, doi:10.1038/s41477-022-01269-w.
APA
Van Leene, J., Eeckhout, D., Gadeyne, A., Matthijs, C., Han, C., De Winne, N., … De Jaeger, G. (2022). Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins. NATURE PLANTS, 8(11), 1245–1261. https://doi.org/10.1038/s41477-022-01269-w
Chicago author-date
Van Leene, Jelle, Dominique Eeckhout, Astrid Gadeyne, Caroline Matthijs, Chao Han, Nancy De Winne, Geert Persiau, et al. 2022. “Mapping of the Plant SnRK1 Kinase Signalling Network Reveals a Key Regulatory Role for the Class II T6P Synthase-like Proteins.” NATURE PLANTS 8 (11): 1245–61. https://doi.org/10.1038/s41477-022-01269-w.
Chicago author-date (all authors)
Van Leene, Jelle, Dominique Eeckhout, Astrid Gadeyne, Caroline Matthijs, Chao Han, Nancy De Winne, Geert Persiau, Eveline Van De Slijke, Freya Persyn, Toon Mertens, Wouter Smagghe, Nathalie Crepin, Ellen Broucke, Daniël Van Damme, Roman Pleskot, Filip Rolland, and Geert De Jaeger. 2022. “Mapping of the Plant SnRK1 Kinase Signalling Network Reveals a Key Regulatory Role for the Class II T6P Synthase-like Proteins.” NATURE PLANTS 8 (11): 1245–1261. doi:10.1038/s41477-022-01269-w.
Vancouver
1.
Van Leene J, Eeckhout D, Gadeyne A, Matthijs C, Han C, De Winne N, et al. Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins. NATURE PLANTS. 2022;8(11):1245–61.
IEEE
[1]
J. Van Leene et al., “Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins,” NATURE PLANTS, vol. 8, no. 11, pp. 1245–1261, 2022.
@article{01GKRHMERBRPENP3VEZPH6VZFE,
  abstract     = {{The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited. Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking. Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation. At the intersection of this multi-dimensional interactome, we discovered a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins. Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1. Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.}},
  author       = {{Van Leene, Jelle and Eeckhout, Dominique and Gadeyne, Astrid and Matthijs, Caroline and Han, Chao and De Winne, Nancy and Persiau, Geert and Van De Slijke, Eveline and Persyn, Freya and Mertens, Toon and Smagghe, Wouter and Crepin, Nathalie and Broucke, Ellen and Van Damme, Daniël and Pleskot, Roman and Rolland, Filip and De Jaeger, Geert}},
  issn         = {{2055-026X}},
  journal      = {{NATURE PLANTS}},
  keywords     = {{Plant Science,TREHALOSE 6-PHOSPHATE,ARABIDOPSIS-THALIANA,NEGATIVE REGULATORS,METABOLIC PATHWAYS,SENSOR SNRK1,PHOSPHORYLATION,COMPLEXES,EVOLUTION,STARCH,LOCALIZATION}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{1245--1261}},
  title        = {{Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins}},
  url          = {{http://doi.org/10.1038/s41477-022-01269-w}},
  volume       = {{8}},
  year         = {{2022}},
}
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