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Project: A study of the factors which influence solubility of eucaryotic proteins in Escherichia coli.

01-JAN-00 – 31-DEC-99

We are able to produce heterologous proteins in E. coli in an amount about 30% of the total cellular proteins. However, an unexpected problem here, is the fact that these proteins are only active when soluble. Therefore in this project, we are looking for factors (such as temperature,...) which have an influence on the solubility of proteins.