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Project: Anisotropic calcium carbonate particles for biosensing and intracellular delivery

project duration
01/01/14 – 31/12/14
abstract
NExt generation of drug delivery carriers necessitate precise control of morphology, porosity, size and loading capacity. Biocompatible calcium carbonate particles represent and ideal system for building an elaborate drug delivery system. Understanding fundamental mechanisms which drive the formation of calcium carbonate particles is a growing area of research. The particles are relatively simple to make, inexpensive, and biocompatible making them an ideal candidate system for industrial applications and eventual applications in hospitals. We shall pursue development of anisotropic calcium carbonate particles with controlled morphology, porosity and size. Such parameters as salts, temperature, ionic strength are expected to influence the particle formation. Loading of paricles with biomolecules along with fabrication of pure protein particles will also be performed. Such particles are expected to provide an enhanced intracellular uptake which is also relevant for the pulmonary drug delivery: therefore, the influence of these parameters on the particle formation will be investigated. We shall also study intracellular uptake of these particles and their toxicity. In addition, porosity of calcium carbonate particles will be used for absorbing or synthesizing noble metal nanoparticles. This part of the project will be devoted to biosensing. Biosensing capabilities are essential for Raman based label-free identification of molecules and intracellular sensing. Developed particles will be used as biosensors for detection of molecules in a solution. Intracellular incorporation of such particles will provide means of investigation of intracellular state and trafficking of proteins.