Project: In situ analysis of protein-protein interactions using a receptor-based detection and screening platforms.
- project duration
- 01-OCT-01 – 30-SEP-03
- MAPPIT (Mammalian Protein-Protein Interaction Trap) allows detecting (phosphotyrosine-dependent) protein interactions in intact human cells. Screening of complex cDNA indicated a low background leven (Eyckerman et al., Nature Cell. Biol. 3, 1114-1119, 2001). In this project, this methodology is extended primarily towards multiple types of modification-dependent protein interactions and towards the development of a drug screening platform. In addition, several screens using a selected set of 'bait'-polypeptides will be performed.