Ghent University Academic Bibliography


Project: Optimisation of the Pichia pastoris expression system for the production of humanized therapeutic glycoproteins

project duration
01-JAN-04 – 31-DEC-07
The goal of the project is triple: (1) we want to construct a set of P. pastoris strains that produce each protein as a single glycoform. (2) we want to enhance the secretory capacity of these strains resulting in acceptable expression levels of proteins known as "hard to produce". (3) we want to study the impact of N-glycosylation on biological properties (in vitro and in vivo) of glycoproteins.