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Project: Development of two-dimensional gel electrophoresis for the systematic identification of plant proteins by means of microsequencing. Identification of fatty acid synthezising proteins in seeds.

01-JAN-00 – 31-DEC-99

The main goal of the project is to elaborate two-dimensional gel electrophoresis to systematically identify minor proteins and membrane-bound proteins. Once the technique is established, it will be used for two discrete applications: the identification of cellular proteins which expression was altered in Arabidopsis mutants and the identification of enzymes involved in the fatty acid biosynthesis in seeds.