Project: Studies of the synergistic effect of cAMP and TNG on the induction of the promotor of the Interleukin-6 gene.
- project duration
- 01-JAN-00 – 31-DEC-99
- The project concerns the detailed analysis of the diverse sequence elements present in the promotor of the IL6gene, and of their cooperative effects upon gene activation by cytokines (in part. TNF)in the presence of absence of additional stimulators,e.gcAMP.The exdperimental approach will be carried out by means of different recombinant constructions (and their mutated variants in the IL6 promotor area), in which a reporter gene will be used for quantification of the expression levels obtained.