Project: Optimisation of MAPPIT, a two-hybrid trap in human cells
- project duration
- 01-DEC-06 – 31-DEC-07
- MAPPIT allows detecting protein-protein interactions in human cells. The method is based on complementation of a signalling-deficient cytokine receptor with a reda-out based on a reporter gene. In this project we will investigate a read-out based on the fosfoSTAT3 signaling moiety using specific antibodies conjugated with fluorofores that excite in the infrared spectrum. This variant procedure will be cost- and time-effective and will open the way to automation of MAPPIT.