Project: In vitro generation of T cells from human hematopoietic stem cell in cord blood, adult mobilised peripheral blood and bone marrow, through modulation of the intensity of the notch signal
- project duration
- 01-OCT-06 – 30-SEP-08
- We will use the OP9 stromal cell line, transduced with different notch ligands, to generate T cells out of human HSC, modulating the intensity of the notch signal though a y-secretase inhibitor. The optimal conditions for cord blood, mobilsed peripheral blood, mobilsed peripheral blood and bone marrow will be tested. Next, we will generate tumour antigen specific T cells in this model.